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Twenty-one novel tri- and tetranucleotide microsatellite loci for the Amur tiger (Panthera tigris altaica)

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Abstract

Amur tiger is the largest subspecies of tiger in the world and his conservation has also received much attention. In this study, we isolated and characterized twenty-one tri- and tetranucleotide microsatellite markers from this species. The number of alleles for each locus ranged from two to nine in a group of 60 individuals and the observed and expected heterozygosities were 0.333–0.917 and 0.302–0.822, respectively. The overall discrimination power and exclusion probabilities in parentage and paternity testing for these markers were 1.00, 0.9947 and 0.9999, respectively, indicating high-resolution power of microsatellite markers.

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Acknowledgements

We thank Xiongsen Bear and Tiger Village of Guilin in China for providing all samples. This work was supported by a special grant from the state forestry administration of China (No. 2005-4-C04).

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Authors and Affiliations

  1. College of Life Sciences, Zhejiang University, Hangzhou, 310058, P.R. China

    Jian-Hui Wu, Yan-Le Lei, Sheng-Guo Fang & Qiu-Hong Wan

  2. State Conservation Center for Gene Resources of Endangered Wildlife, Zhejiang University, Hangzhou, 310058, P.R. China

    Jian-Hui Wu, Yan-Le Lei, Sheng-Guo Fang & Qiu-Hong Wan

  3. Key Laboratory of Conservation Genetics and Reproductive Biology for Endangered Wild Animals of the Ministry of Education, Zhejiang University, Hangzhou, 310058, P.R. China

    Jian-Hui Wu, Yan-Le Lei, Sheng-Guo Fang & Qiu-Hong Wan

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  1. Jian-Hui Wu
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  2. Yan-Le Lei
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  3. Sheng-Guo Fang
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  4. Qiu-Hong Wan
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Correspondence to Qiu-Hong Wan.

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Wu, JH., Lei, YL., Fang, SG. et al. Twenty-one novel tri- and tetranucleotide microsatellite loci for the Amur tiger (Panthera tigris altaica). Conserv Genet 10, 567–570 (2009). https://doi.org/10.1007/s10592-008-9571-8

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  • DOI: https://doi.org/10.1007/s10592-008-9571-8

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