Technical Note

Conservation Genetics

, Volume 10, Issue 3, pp 567-570

Twenty-one novel tri- and tetranucleotide microsatellite loci for the Amur tiger (Panthera tigris altaica)

  • Jian-Hui WuAffiliated withCollege of Life Sciences, Zhejiang UniversityState Conservation Center for Gene Resources of Endangered Wildlife, Zhejiang UniversityKey Laboratory of Conservation Genetics and Reproductive Biology for Endangered Wild Animals of the Ministry of Education, Zhejiang University
  • , Yan-Le LeiAffiliated withCollege of Life Sciences, Zhejiang UniversityState Conservation Center for Gene Resources of Endangered Wildlife, Zhejiang UniversityKey Laboratory of Conservation Genetics and Reproductive Biology for Endangered Wild Animals of the Ministry of Education, Zhejiang University
  • , Sheng-Guo FangAffiliated withCollege of Life Sciences, Zhejiang UniversityState Conservation Center for Gene Resources of Endangered Wildlife, Zhejiang UniversityKey Laboratory of Conservation Genetics and Reproductive Biology for Endangered Wild Animals of the Ministry of Education, Zhejiang University
  • , Qiu-Hong WanAffiliated withCollege of Life Sciences, Zhejiang UniversityState Conservation Center for Gene Resources of Endangered Wildlife, Zhejiang UniversityKey Laboratory of Conservation Genetics and Reproductive Biology for Endangered Wild Animals of the Ministry of Education, Zhejiang University Email author 

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Abstract

Amur tiger is the largest subspecies of tiger in the world and his conservation has also received much attention. In this study, we isolated and characterized twenty-one tri- and tetranucleotide microsatellite markers from this species. The number of alleles for each locus ranged from two to nine in a group of 60 individuals and the observed and expected heterozygosities were 0.333–0.917 and 0.302–0.822, respectively. The overall discrimination power and exclusion probabilities in parentage and paternity testing for these markers were 1.00, 0.9947 and 0.9999, respectively, indicating high-resolution power of microsatellite markers.

Keywords

Amur tiger Microsatellite Polymorphic Exclusionary power