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Expression of genes related to flavonoid and stilbene synthesis as affected by signaling chemicals and Botrytis cinerea in grapevines

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Biologia Plantarum

Abstract

Recent studies have shown that the expression of genes related to the synthesis of flavonoids, such as the phenylalanineammonia lyase (PAL), chalcone synthase (CHS), chalcone isomerase (CHI), and stilbene synthase (STS) genes were induced in response to different signaling molecules and Botrytis cinerea inoculation in grapevine leaves. Therefore, in the present study, the nucleotide and deduced amino acid sequences of STS genes from cultivars Campbell Early and Kyoho were compared. The deduced amino acid sequences of VlKSTS12, VlKSTS11, VICESTS13, and VlKSTS1 showed 100 % homology to VlCESTS12, VlCESTS11, VICESTS24, and VlKSTS13, respectively, in Campbell Early and Kyoho. In addition, the maximum transcription was observed 6 h after chemical treatments. In Campbell Early, the transcription of PAL, CHS, and CHI was higher in leaves treated with ethephon than in those treated with hydrogen peroxide, methyl jasmonate, and salicylic acid. The PAL, CHS, and CHI genes were more induced in Campbell Early than in Kyoho. The mRNA content of STS genes started to increase at 6 h and peaked at 48 h after the treatments. In Kyoho leaves, the expression of STSs was highly up-regulated at 1 h and peaked at 6 h after the treatments. The expression of the STS genes was induced in both the cultivars in leaves inoculated with B. cinerea. STS11 and STS12 showed differential expression patterns from STS1, STS24, and STS13 in Campbell Early leaves inoculated with B. cinerea.

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Abbreviations

CHI:

chalcone isomerase

CHS:

chalcone synthase

ET:

ethephon

FLS:

flavonol synthase

GABA:

γ-aminobutyric acid

MeJA:

methyl jasmonate

PAL:

phenylalanineammonia lyase

PR:

pathogenesis-related

SA:

salicylic acid

STS:

stilbene synthase

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Authors and Affiliations

  1. Department of Horticulture and Life Science, Yeungnam University, Gyeongsan, 712-749, South Korea

    S. Y. Ahn, S. A. Kim & H. K. Yun

  2. Highland Agriculture Research Center, National Institute of Crop Science, Pyeongchang, 232-955, South Korea

    K. S. Cho

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  1. S. Y. Ahn
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  2. S. A. Kim
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  4. H. K. Yun
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Correspondence to H. K. Yun.

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Acknowledgements: This work was supported by a grant from the Next-Generation BioGreen 21 program (no.PJ008213), Rural Development Administration, the Republic of Korea.

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Ahn, S.Y., Kim, S.A., Cho, K.S. et al. Expression of genes related to flavonoid and stilbene synthesis as affected by signaling chemicals and Botrytis cinerea in grapevines. Biol Plant 58, 758–767 (2014). https://doi.org/10.1007/s10535-014-0437-2

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  • DOI: https://doi.org/10.1007/s10535-014-0437-2

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