Biologia Plantarum

, Volume 56, Issue 4, pp 771–774

Stomatal NH3 compensation point and its metabolic regulation in senescence phenotypes of Nicotiana tabacum

  • W. J. Duan
  • T. Z. Yang
  • Y. Dai
  • D. L. Li
  • X. Q. Zhang
  • H. B. Liu
  • N. Li
  • C. G. Wang
Brief Communication

DOI: 10.1007/s10535-012-0141-z

Cite this article as:
Duan, W.J., Yang, T.Z., Dai, Y. et al. Biol Plant (2012) 56: 771. doi:10.1007/s10535-012-0141-z

Abstract

We compared stomatal ammonia compensation point (χs) and its metabolic regulation in tobacco (Nicotiana tabacum) leaves of a quick-leaf-senescence phenotype ZY90 and a slow-leaf-senescence phenotype NC89. Compared with NC89, ZY90 had significantly higher χs values between 40 and 60 d after leaf sprouting in spite of its lower nitrogen content. During the same time, a steeper decline in glutamine synthetase activity was detected in ZY90 leaves, simultaneously with a steep increase in χs. These results suggested that the quick leaf senescence phenotype exhibited high NH3 emission potential due to efficient nitrogen recycling and remobilization, and glutamine synthetase played a key role in regulating χs in ZY90.

Additional key words

glutamine synthetasenitrogen contentquick- and slow-leaf-senescence phenotypestobacco

Abbreviations

DAS

days after leaf sprouting

GDH

glutamate dehydrogenase

GS

glutamine synthetase

[H+]apo

apoplastic H+ content

[NH4+]apo

apoplastic NH4+ content

pHapo

apoplastic pH

χs

ammonia stomatal compensation point

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • W. J. Duan
    • 1
    • 2
  • T. Z. Yang
    • 1
  • Y. Dai
    • 2
  • D. L. Li
    • 2
  • X. Q. Zhang
    • 1
  • H. B. Liu
    • 1
  • N. Li
    • 2
  • C. G. Wang
    • 2
  1. 1.Henan Agricultural UniversityZhengzhouP.R.China
  2. 2.Research and Development CenterChina Tobacco Chuanyu Industrial CorporationChengduP.R. China