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Oceanibacterium hippocampi gen. nov., sp. nov., isolated from cutaneous mucus of wild seahorses (Hippocampus guttulatus)

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Abstract

A Gram-negative, aerobic, motile and slightly curved rod-shaped bacterium (BFLP-8T) was isolated from cutaneous mucus of wild long-snouted seahorses (Hippocampus guttulatus) captured in northwest Spain (Toralla, Galicia). Strain BFLP-8T grew at 10–35 °C and pH 5–9 (optimally at 25 °C and pH 7.0) and with 1–6 % (w/v) NaCl (optimally with 2 % NaCl). The predominant respiratory quinone (90 %) was ubiquinone with ten isoprene units (Q-10) and the major fatty acids identified were C18:1 ω7c (54.8 % of the total), C19:0 cyclo ω8c (11.6 %), C16:0 (9.5 %), C18:1 2-OH (7.1 %) and C16:1 ω11c (6.7 %). The G+C content of the DNA was 57.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BFLP-8T formed a distinct clade within the family Sneathiellaceae but is not specifically associated with any species in the family. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain BFLP-8T represents a novel species within a new genus, for which the name Oceanibacterium hippocampi gen. nov., sp. nov. is proposed. The type strain is BFLP-8T (=CECT 7691T = DSM 23444T).

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Acknowledgments

This study was financed by the Spanish Ministry of Science and Innovation (Projects CGL2005-05927-C03-01 and CGL2009-08386) and by the Regional Government Xunta de Galicia (09MDS022402PR). J. L. B. was supported by a postdoctoral I3P contract from the Spanish National Research Council (CSIC), co-financed by the European Social Fund. We thank P. Quintas, A. Chamorro, M. Cueto, S. Otero and P. Ruiz for skilful technical assistance.

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Authors and Affiliations

  1. Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas (IIM-CSIC), 36208, Vigo, Spain

    José Luis Balcázar, Miquel Planas & José Pintado

  2. Catalan Institute for Water Research (ICRA), Scientific and Technological Park of the University of Girona, 17003, Girona, Spain

    José Luis Balcázar

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  1. José Luis Balcázar
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  2. Miquel Planas
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  3. José Pintado
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Correspondence to José Luis Balcázar.

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Balcázar, J.L., Planas, M. & Pintado, J. Oceanibacterium hippocampi gen. nov., sp. nov., isolated from cutaneous mucus of wild seahorses (Hippocampus guttulatus). Antonie van Leeuwenhoek 102, 187–191 (2012). https://doi.org/10.1007/s10482-012-9726-y

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