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Saccharothrix yanglingensis sp. nov., an antagonistic endophytic actinomycete isolated from cucumber plant

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Abstract

An endophytic actinomycete strain, designated Hhs.015T, was isolated from roots of cucumber seedlings. The endophytic isolate was identified by means of a polyphasic taxonomic approach. On the basis of 16S rRNA gene sequence similarities, strain Hhs.015T was closely related to members of the genus Saccharothrix. DNA–DNA hybridization with the four closest relatives, Saccharothrix longispora NRRL B-16116T, Saccharothrix xinjiangensis NRRL B-24321T, Saccharothrix autraliensis CGMCC 4.1355T and Saccharothrix espanaensis CGMCC 4.1714T, gave similarity values of 33.8, 28.2, 44.1 and 29.5%, respectively, which indicated that strain Hhs.015T represents a novel species of the genus Saccharothrix. This is consistent with the morphological, physiological and chemotaxonomic data. As a whole, these results suggest that strain Hhs.015T represents a novel Saccharothrix species. The name Saccharothrix yanglingensis sp. nov. is proposed, with the type strain Hhs.015T (=CGMCC 4.5627T = KCTC 19722T).

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Acknowledgments

This work was supported by Agriculture Project (nyhyzx07–055), National Natural Science Foundation of China (30771396) and the ‘111’ Project from Ministry of Education of China (B07049). The authors are grateful to Dr. Swezey (NRRL) for the type strains of the Saccharothrix species, and to Dr. Yafang Tan for carrying out the quantitative fatty acid analysis on isolate Hhs.015T.

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Authors and Affiliations

  1. State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Xia Yan, Li-Li Huang, Xuan Tu, Xiao-Ning Gao & Zhen-Sheng Kang

  2. College of Plant Protection, Northwest A&F University, Yangling, 712100, China

    Li-Li Huang, Xuan Tu, Xiao-Ning Gao & Zhen-Sheng Kang

  3. College of Life Sciences, Northwest A&F University, Yangling, 712100, China

    Xia Yan

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  1. Xia Yan
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  2. Li-Li Huang
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  3. Xuan Tu
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  4. Xiao-Ning Gao
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  5. Zhen-Sheng Kang
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Correspondence to Li-Li Huang or Zhen-Sheng Kang.

Additional information

The GenBank accession number for the 16S rRNA gene sequence of strain Hhs.015T is GQ284639.

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Yan, X., Huang, LL., Tu, X. et al. Saccharothrix yanglingensis sp. nov., an antagonistic endophytic actinomycete isolated from cucumber plant. Antonie van Leeuwenhoek 101, 141–146 (2012). https://doi.org/10.1007/s10482-011-9631-9

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  • DOI: https://doi.org/10.1007/s10482-011-9631-9

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