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Tunicatimonas pelagia gen. nov., sp. nov., a novel representative of the family Flammeovirgaceae isolated from a sea anemone by the differential growth screening method

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Abstract

A Gram-negative, strictly aerobic, reddish-pink pigmented, non-motile, rod-shaped strain designated N5DB8-4T, was isolated from an orange-striped sea anemone Diadumene lineata by a differential growth screening method. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that the novel isolate was affiliated with the family Flammeovirgaceae of the phylum Bacteroidetes and that it showed highest sequence similarity (89.1%) to Porifericola rhodea N5EA6-3A2BT. The strain could be differentiated phenotypically from recognized members of the family Flammeovirgaceae. The G+C content of the DNA is 52.6 mol%, the major respiratory quinone is menaquinone 7 (MK-7) and iso-C15:0, C16:1ω5c and iso-C15:1 G (the double-bond position indicated by capital letter is unknown) were the major fatty acids. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the strain represents a novel taxon for which the name Tunicatimonas pelagia gen. nov., sp. nov. is proposed. The type strain of Tunicatimonas pelagia is N5DB8-4T (=KCTC 23473T = NBRC 107804T).

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Acknowledgment

The present research has been conducted by the Settlement Research Grant of Keimyung University in 2011.

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Author notes

  1. Naoya Oku

    Present address: Biotechnology Research Center, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama, 939-0398, Japan

  2. Atsuko Katsuta & Hiroaki Kasai

    Present address: Marine Biosciences Kamaishi Research Laboratory, Kitasato University, 3-75-1 Heita, Kamaishi, Iwate, 026-0001, Japan

Authors and Affiliations

  1. College of Pharmacy, Keimyung University, 2800 Dalgubeoldaero, Dalseo-Gu, Daegu, 704-701, Republic of Korea

    Jaewoo Yoon

  2. Marine Biotechnology Institute Co. Ltd, 3-75-1 Heita, Kamaishi, Iwate, 026-0001, Japan

    Naoya Oku, Atsuko Katsuta & Hiroaki Kasai

  3. Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo, 113-0032, Japan

    Sanghwa Park

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  1. Jaewoo Yoon
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Correspondence to Jaewoo Yoon.

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Yoon, J., Oku, N., Park, S. et al. Tunicatimonas pelagia gen. nov., sp. nov., a novel representative of the family Flammeovirgaceae isolated from a sea anemone by the differential growth screening method. Antonie van Leeuwenhoek 101, 133–140 (2012). https://doi.org/10.1007/s10482-011-9626-6

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