Skip to main content
SpringerLink
Log in

Purification and Preparation of Moenomycin A from Fermentation Broth by Multidimensional Chromatography

  • Original
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

Based on various adsorption characteristics of resins, a novel method for purifying and preparing moenomycin A from fermentation broth was established by combining different chromatographic modes into a three-step preparative chromatography process. Fermentation broth of moenomycins was firstly prepurified by macroporous adsorbent XAD7HP to remove most strong polar impurities, then further purified by anion exchange resin FPA98Cl, and finally refined moenomycin A was obtained by use of semi-preparative reversed-phase chromatographic column packed with Chromtorex C8 silica gel. As the main indicators, purity and yield of moenomycin A were examined in order to optimise the chromatographic process for each step. Under optimized chromatographic conditions, the purity and total yield of moenomycin A were 95.0 and 22.2 %, and the biological potency of moenomycin A was 2232 U mg−1, significantly higher than 1395 U mg−1, which is the potency of the standard from Agriculture Ministry of China. Three-step preparative chromatographic mode could gradually and effectively remove impurities. The present method is practical, easy to be operated with less solvent consumption, and provides a new idea for the preparation of moenomycin A with high purity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. Huber G, Nesemann G (1968) Biochem Riophys Res Commun 30(1):7–13

    Article  CAS  Google Scholar 

  2. Lindner F, Wallhausser K, Huber G (1972) US Pat Appl 3674866 A, pp 1–5

  3. Butaye P, Devriese LA, Haesebrouck F (2003) Clin Microbiol Rev 16(2):175–188

    Article  CAS  Google Scholar 

  4. Edwards JE, Mcewan NR, Nest MK, Nicola W, John WR (2005) Microbiol 151:717–725

    Article  CAS  Google Scholar 

  5. Parks CW, Grimes JL, Ferket PR, Fairchild AS (2001) Poult Sci 80:718–723

    Article  CAS  Google Scholar 

  6. Bauer F, Huber G, Karl HW (1966) US Pat Appl 3279923, pp 1–4

  7. Kempin U, Hennig L, Knoll D, Welzel P, Müller D, Markus A, Heijenoord JV (1997) Tetrahedron 53:17669–17690

    Article  CAS  Google Scholar 

  8. Vertesy L, Kurz M, Markus A, Seibert G (2000) US Pat Appl 6077830, pp 1–9

  9. Riess G, Seibert G, Hedtmann U (2001) US Pat Appl 6242424 B1, pp 1–6

  10. Wallhausser KH, Nesemann G, Prave P, Steigler A (1965) Antimicrob Agents Chemother 5:734–736

    CAS  Google Scholar 

  11. Huang CY, Shih HW, Lin LY, Tien YW, Cheng TJR, Cheng WC, Wong CH, Ma C (2012) PNAS 109(17):6496–6501

    Article  CAS  Google Scholar 

  12. Yuan Y, Fuse S, Ostash B, Sliz P, Kahne D, Walker S (2008) ACS Chem Bio 3(7):429–436

    Article  CAS  Google Scholar 

  13. Heaslet H, Shaw B, Mistry A, Miller AA (2009) Struct Biol 167:129–135

    Article  CAS  Google Scholar 

  14. Sung MT, Lai YT, Huang CY, Chou LY, Shih HW, Cheng WC, Wong CH, Ma C (2009) PNAS 106(22):8824–8829

    Article  CAS  Google Scholar 

  15. Lovering AL, Castro LHD, Daniel L, Strynadka NCJ (2007) Science 315:1402–1405

    Article  CAS  Google Scholar 

  16. Zehl M, Pittenauer E (2006) J Am Soc Mass Spectrom 17:1081–1090

    Article  CAS  Google Scholar 

  17. Huber G (1967) A purified form of the antibiotic Moenomycin and a process for its manufacture. Pat Off UK GB1068639 pp 1–6

  18. Zheng YH, Li WX, Zhang GD, Qi P, Xiao J, Yang XY, Zhao H (2005) State Intellect Prop Off PRC CN1683549A, pp 1–3

  19. Vertesy L, Stark A, Ehlers E (1999) US Pat Appl 5986089 A, pp 1–3

  20. Duphorn I, Schacht U, Tschesche R (1972) US Pat Appl 3660569, pp 1–3

  21. Sun CH, Jiang W, Zou YJ, Nie SJ, Jin WZ (2003) Chin J Antibiot 28(6):325–327

    CAS  Google Scholar 

  22. Li XL, Xi CX, Wang GM, Li YG, Tang BB, Zhang L (2012) State Intellect Prop Off PRC CN102603817A, pp 1–4

  23. Xu H, Zhang HW, Wang FM, Zhang XM, Cai X (2014) J Food Saf Qual 5(12):3784–3789

    Google Scholar 

  24. Tian LL, Huang DM, Shi YF, Han F, Zhou P (2015) Determination flavomycin residue in grass carp by high performance liquid chromatography. 3rd International Conference on Material, Mechanical and Manufacturing Engineering, pp 462–465

  25. Pasquale G, Serena F, Luigi S, Maurizio F, Cinzia C, Paolo S (2010) Rapid Commun Mass Spectrom 24(7):1017–1024

    Article  Google Scholar 

  26. Kurz M, Guba W, Vértesy L (1998) Eur J Bio Chem 252:500–507

    Article  CAS  Google Scholar 

  27. Chang JH, Guo L (1997) Separation and purification of organic compounds. Shaanxi Science and Technology Press, Xi’an, Shaanxi, pp 184–185

  28. Lantzsch G, Binder H, Heerklotz H, Welzel P, Klose G (1998) Langmuir 14(15):4095–4104

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Chemical Engineering Research Center of the Ministry of Education for Advanced Use Technology of Shanbei Energy, School of Chemical Engineering, Northwest University, Xi’an, 710069, Shaanxi, People’s Republic of China

    Bin Chen, Rong Li, Yanbin Guo, Guoliang Chen & Xiaoxun Ma

  2. School of Natural and Applied Sciences, Northwestern Polytechnical University, Xi’an, 710072, Shaanxi, People’s Republic of China

    Kaidi Yang

Authors
  1. Bin Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rong Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yanbin Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kaidi Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Guoliang Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Xiaoxun Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Rong Li.

Ethics declarations

Funding

This study was funded by the grants from the National Natural Science Foundation of China (No. 21376191), Services Local Special Program of Shaanxi Province Educational Department (No. 14JF027), Natural Science Foundation of Shaanxi Province (No. 2011JM2013), and Industrialization Cultivation Item of Shaanxi Province Educational Department (No. 2013JC23).

Conflict of interest

The authors declared that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chen, B., Li, R., Guo, Y. et al. Purification and Preparation of Moenomycin A from Fermentation Broth by Multidimensional Chromatography. Chromatographia 79, 667–674 (2016). https://doi.org/10.1007/s10337-016-3086-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10337-016-3086-0

Keywords

Search

Navigation