Abstract
A simple and sensitive method of gas chromatography–mass spectrometry for quantification of β-elemene in human plasma was developed and validated. An n-hexane-based liquid–liquid extraction assisted by saturated sodium chloride solution was used for sample preparation. Naphthalene was chosen as the internal standard. The calibration curve (r 2 = 0.9997) of β-elemene was established at the range of 0.0655–40.00 μg mL−1 and the lower limit of quantification (LLOQ) was 0.0655 μg mL−1 using 200 μL of plasma, respectively. The accuracy was in a range of 87.1–101.7 %, and intra- and inter-day precisions (RSD %) at three QC levels were all less than 10 %. The LLOQ of 0.0665 μg mL−1 is sensitive enough to perform pharmacokinetic studies after β-elemene administration.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (No. 81102499), Hunan Science and Technology Project (No. 2011SK3261) and the Fundamental Research Funds for the Central Universities of Central South University (No. 2014zzts313).
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S. Li and R. Zhou have contributed equally.
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Li, S., Zhou, R., Mu, L. et al. A Sensitive GC–MS Method for the Determination of β-Elemene in Human Plasma. Chromatographia 78, 729–734 (2015). https://doi.org/10.1007/s10337-015-2875-1
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DOI: https://doi.org/10.1007/s10337-015-2875-1