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Accurate Mass Determination of Platycosides Prepared by Macroporous Resins Using High-Resolution Mass Spectroscopy and Optimization Methods

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Abstract

The method using macroporous resin for isolation of platycosides from Platycodi radix extract was developed. The platycosides were detected and analyzed using quadrupole time-of-flight mass spectroscopy in negative ion mode. The employment of chemometric methods effectively reduced the influence of signal intensity or noise on the mass accuracy. Finally, the monoisotopic masses were accurately measured, and relative isotopic abundances of the ions were further investigated. The compound match was obtained by comparison with a specific monomer’s database, and the optimal results allow researchers to narrow their database search window. In addition, the approximate elution order of some isomers was determined via comparison with mass spectrum and chemical structure analysis.

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References

  1. Nyakudya E, Jeong JH, Lee NK, Jeong YS (2014) Prev Nutr Food Sci 19:59–68

    Article  CAS  Google Scholar 

  2. Liu F, Wu B, Xiao W, Wang ZZ, Sun ZL, Yin J, Huang CG (2013) Chromatographia 76:1131–1139

    Article  CAS  Google Scholar 

  3. Vergeynst L, Van Langenhove H, Joos P, Demeestere K (2013) Anal Chim Acta 789:74–82

    Article  CAS  Google Scholar 

  4. Pluskal T, Uehara T, Yanagida M (2012) Anal Chem 84:4396–4403

    Article  CAS  Google Scholar 

  5. Li DD, Xue R, Li ZX, Chen MC, Jiang WX, Huang CG (2014) Chromatographia 77:853–858

    Article  CAS  Google Scholar 

  6. Guo JM, Xue CF, Duan JA, Shang EX, Qian DW, Tang YP, Ouyang Q, Sha M (2011) Chromatographia 73:447–456

    Article  CAS  Google Scholar 

  7. Zhao H, Wu X, Wang H, Gao B, Yang J, Si N, Bian B (2013) J Sep Sci 36:492–502

    Article  CAS  Google Scholar 

  8. Cox J, Mann M (2009) J Am Soc Mass Spectrom 20:1477–1485

    Article  CAS  Google Scholar 

  9. Mihaleva VV, Vorst O, Maliepaard C, Verhoeven HA, de Vos RCH, Hall RD, van Ham RCHJ (2008) Metabolomics 4:171–182

    Article  CAS  Google Scholar 

  10. Cappellin L, Biasioli F, Fabris A, Schuhfried E, Soukoulis C, Märk TD, Gasperi F (2010) Int J Mass Spectrom 290:60–63

    Article  CAS  Google Scholar 

  11. Ye F, Yang R, Hua X, Zhao G (2014) Food Chem 159:38–46

    Article  CAS  Google Scholar 

  12. Wang C, Chao Z, Sun W, Wu X, Ito Y (2014) J Liq Chromatogr Relat Technol 37:572–587

    Article  CAS  Google Scholar 

  13. Xu BJ, Zheng YN (2000) J Dalian Univ 21:77–79 (In Chinese)

    Google Scholar 

  14. Yang XW, Liu MX, Liu GY, Li SM, Yang Q (2002) J Yangzhou Univ 23:15–17 (In Chinese)

    Google Scholar 

  15. Huang Y, Shi CL, Liu MX, Yu HY (2008) J Yangzhou Univ 11:41–44 (In Chinese)

    CAS  Google Scholar 

  16. Liu MX, Li WH, Li D, Qiao J, Lin L, Gao R (2009) Chin Tradit Pat Med 31:130–133 (In Chinese)

    CAS  Google Scholar 

  17. He M, Li Y, Yan J, Cao D, Liang Y (2013) J Chromatogr Sci 51:318–330

    Article  CAS  Google Scholar 

  18. He M, Lv HY, Li YP (2014) Vicente Gonçalves CM, Dong NP, Pan LS, Liu PL, Liang YZ. Anal Methods 6:2239–2246

    Article  CAS  Google Scholar 

  19. Fernandez-de-Cossio Diaz J, Fernandez-de-Cossio J (2012) Anal Chem 84:7052–7056

  20. Knolhoff AM, Callahan JH, Croley TR (2014) J Am Soc Mass Spectrom 25:1285–1294

    Article  CAS  Google Scholar 

  21. Jiang W, Erve JC (2012) Rapid Commun Mass Spectrom 26:1014–1022

    Article  CAS  Google Scholar 

  22. Grimme S (2013) Angew Chem Int Ed Engl 52:6306–6312

    Article  CAS  Google Scholar 

  23. Yoo DS, Choi YH, Cha MR, Lee BH, Kim SJ, Yon GH, Hong KS, Jang YS, Lee HS, Kim YS, Ryu SY, Kang JS (2011) Food Chem 129:645–651

    Article  CAS  Google Scholar 

  24. Ha YW, Na YC, Seo JJ, Kim SN, Linhardt RJ, Kim YS (2006) J Chromatogr A 1135:27–35

    Article  CAS  Google Scholar 

  25. Na YC, Ha YW, Kim YS, Kim KJ (2008) J Chromatogr A 1189:467–475

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work is financially supported by the general project of Hunan Provincial Education Department (Grants No. 14C1098, Grant No. 11B128) and Xiang Tan University Research Fund (No. 13QDZ34). The studies meet with the approval of the university’s review board.

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Authors and Affiliations

  1. Department of Pharmaceutical Engineering, School of Chemical Engineering, Xiangtan University, Xiangtan, 411105, People’s Republic of China

    Min He & Juan Nie

  2. Institute of Forensic Science, Hunan Provincial Public Security Bureau, Changsha, 410001, People’s Republic of China

    Hai Wu

  3. Research Center of Modernization of Chinese Medicines, Central South University, Changsha, 410083, People’s Republic of China

    Yizeng Liang

Authors
  1. Min He
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  2. Juan Nie
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  3. Hai Wu
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  4. Yizeng Liang
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Corresponding authors

Correspondence to Min He or Hai Wu.

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He, M., Nie, J., Wu, H. et al. Accurate Mass Determination of Platycosides Prepared by Macroporous Resins Using High-Resolution Mass Spectroscopy and Optimization Methods. Chromatographia 78, 683–693 (2015). https://doi.org/10.1007/s10337-015-2874-2

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  • DOI: https://doi.org/10.1007/s10337-015-2874-2

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