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Rapid Determination of Uric Acid in Human Urine by CE with an Improved Electromagnetic Induction Detector

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Abstract

A novel and rapid method was developed for the determination of uric acid in human urine by capillary electrophoresis with an improved electromagnetic induction detector. Electrophoretic parameters affecting the separation efficiency, such as buffer composition, buffer pH, buffer concentration, and electroosmotic flow modifier, were systematically investigated. An electrolyte solution consisting of 8.5 mmol L−1 tris(hydroxymethyl)aminomethane (Tris), 1.5 mmol L−1 citric acid, and 0.2 mmol L−1 cetyltrimethylammonium bromide, pH 8.0, was found to be suitable for sample determination. Uric acid was separated and detected within 2.3 min, with a linear response range from 5 to 400 µg mL−1 and a correlation coefficient of 0.9998. Intraday and interday precision were 1.6 and 2.5 % (n = 6), respectively. The recoveries were between 98.3 and 100.5 %. This simple, effective, and stable method is a good alternative to existing methods for uric acid determination. Also, the improved electromagnetic induction detector holds great promise in clinical analysis.

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Acknowledgments

Financial support from the National Natural Science Foundation of China (NSFC, Grant Nos. 81302738, 21375152, and 20727006) is gratefully acknowledged.

Conflict of interest

The authors have declared no conflict of interest.

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Authors and Affiliations

  1. School of Pharmaceutical Sciences, Guangzhou Higher Education Mega Center, Sun Yat-sen University, 132 Waihuan East Road, Guangzhou, 510006, Guangdong, People’s Republic of China

    Xiujuan Yang, Cui Liu & Zuanguang Chen

  2. Department of Pharmacy, Zhujiang Hospital of Southern Medical University, No.253, Industrial Road, Haizhu District, Guangzhou, 510282, Guangdong, People’s Republic of China

    Xiujuan Yang

Authors
  1. Xiujuan Yang
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  2. Cui Liu
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  3. Zuanguang Chen
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Correspondence to Zuanguang Chen.

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Yang, X., Liu, C. & Chen, Z. Rapid Determination of Uric Acid in Human Urine by CE with an Improved Electromagnetic Induction Detector. Chromatographia 78, 119–123 (2015). https://doi.org/10.1007/s10337-014-2807-5

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  • DOI: https://doi.org/10.1007/s10337-014-2807-5

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