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Preparation of PLGA microspheres with different porous morphologies

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Abstract

Poly(D,L-lactide-co-glycolide) (PLGA) microspheres were prepared by emulsion solvent evaporation method. The influences of inner aqueous phase, organic solvent, PLGA concentration on the morphology of microspheres were studied. The results showed that addition of porogen or surfactants to the inner aqueous phase, types of organic solvents and polymer concentration affected greatly the microsphere morphology. When dichloromethane was adopted as organic solvent, microspheres with porous structure were produced. When ethyl acetate served as organic solvent, two different morphologies were obtained. One was hollow microspheres with thin porous shell under a lower PLGA concentration, another was erythrocyte-like microspheres under a higher PLGA concentration. Three types of microspheres including porous, hollow core with thin porous shell (denoted by hollow in brief) and solid structures were finally selected for in vitro drug release tests. Bovine serum albumin (BSA) was chosen as model drug and encapsulated within the microspheres. The BSA encapsulation efficiency of porous, hollow and solid microspheres was respectively 90.4%, 79.8% and 0. And the ultimate accumulative release was respectively 74.5%, 58.9% and 0. The release rate of porous microspheres was much slower than that of hollow microspheres. The experiment results indicated that microspheres with different porous structures showed great potentials in controlling drug release behavior.

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References

  1. Yu, D., Zhang, Y., Zhou, X., Mao, Z. and Gao, C., Biomacromolecules, 2012, 13: 3272

    Article  CAS  Google Scholar 

  2. Chun, K.W., Yoo, H.S., Yoon, J.J. and Park, T.G., Biotechnol. Prog., 2004, 20: 1797

    Article  CAS  Google Scholar 

  3. Shi, X.D., Sun, L., Jiang, J., Zhang, X., Ding, W. and Gan, Z.H., Macromol. Biosci., 2009, 9: 1211

    Article  CAS  Google Scholar 

  4. Yang, Y.Y., Chung, T.S. and Ng, N.P., Biomaterials, 2001, 22: 231

    Article  CAS  Google Scholar 

  5. Luo, R., Neu, B. and Venkatraman, S.S., Small, 2012, 8: 2585

    Article  CAS  Google Scholar 

  6. O’Donnell, P.B. and McGinity, J.W., Eur. J. Pharm. Biopharm., 1998, 45: 83

    Article  Google Scholar 

  7. Bae, S.E., Son, J.S., Park, K. and Han, D.K., J. Control. Release, 2009, 133: 37

    Article  CAS  Google Scholar 

  8. Morita, T., Horikiri, Y., Suzuki, T. and Yoshino, H., Eur. J. Pharm. Biopharm., 2001, 51: 45

    Article  CAS  Google Scholar 

  9. Shi, X.D., Sun, L. and Gan, Z.H., Acta Polymerica Sinica (in Chinese), 2011, (8): 866

  10. Shi, X.D., Jiang, J., Sun, L. and Gan, Z.H., Colloids Surf. B Biointerfaces, 2011, 85: 73

    Article  CAS  Google Scholar 

  11. Sah, H. and Lee, B.J., Macromol. Rapid Commun., 2006, 27: 1845

    Article  CAS  Google Scholar 

  12. Newman, K.D. and McBurney, M.W., Biomaterials, 2004, 25: 5763

    Article  CAS  Google Scholar 

  13. Gabler, F., Frauenschuh, S., Ringe, J., Brochhausen, C., Goetz, P., Kirkpatrick, C.J., Sittinger, M., Schubert, H. and Zehbe, R., Biomol. Eng., 2007, 24: 515

    Article  CAS  Google Scholar 

  14. Wang, Q., Jamal, S., Detamore, M.S. and Berkland, C., J. Biomed. Mater. Res. Part A, 2001, 96A: 520

    Article  Google Scholar 

  15. Hariharan, S., Bhardwaj, V., Bala, I., Sitterberg, J., Bakowsky, U. and Kumar, M., Pharm. Res., 2006, 23: 184

    Article  CAS  Google Scholar 

  16. Jiang, W.L., Gupta, R.K., Deshpande, M.C. and Schwendeman, S.P., Adv. Drug Deliver. Rev., 2005, 57: 391

    Article  CAS  Google Scholar 

  17. Ke, C.J., Su, T.Y., Chen, H.L., Liu, H.L., Chiang, W.L., Chu, P.C., Xia, Y. and Sung, H.W., Angew. Chem. Int. Ed., 2011, 50: 8086

    Article  CAS  Google Scholar 

  18. Kong, S.D., Zhang, W., Lee, J.H., Brammer, K., Lal, R., Karin, M. and Jin, S., Nano Lett., 2010, 10: 5088

    Article  CAS  Google Scholar 

  19. Chiang, W.L., Ke, C.J., Liao, Z.X., Chen, S.Y., Chen, F.R., Tsai, C.Y., Xia, Y. and Sung, H.W., Small, 2010, 8: 3584

    Article  Google Scholar 

  20. Jaklenec, A., Wan, E., Murray, M.E. and Mathiowitz, E., Biomaterials, 2008, 29: 185

    Article  CAS  Google Scholar 

  21. Cheung, H.Y., Lau, K.T., Lu, T.P. and Hui, D., Compos. Part B Eng., 2007, 38: 291

    Article  Google Scholar 

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Authors and Affiliations

  1. State Key Laboratory of Chemical Resource Engineering, Beijing Key Laboratory of Electrochemical Process and Technology for Materials, Beijing University of Chemical Technology, Beijing, 100029, China

    Shu-ying Wang & Feng Wang  (王峰)

  2. Institute of Chemistry, Chinese Academy of Science, Beijing, 100190, China

    Xu-dong Shi & Zhi-hua Gan  (甘志华)

  3. State Key Laboratory of Organic-inorganic Composites, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing, 100029, China

    Zhi-hua Gan  (甘志华)

Authors
  1. Shu-ying Wang
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  2. Xu-dong Shi
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  3. Zhi-hua Gan  (甘志华)
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  4. Feng Wang  (王峰)
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Corresponding authors

Correspondence to Zhi-hua Gan  (甘志华) or Feng Wang  (王峰).

Additional information

This work was financially supported by the National Natural Science Foundation of China (Nos. 51003109, 51125007 and 51025314).

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Wang, Sy., Shi, Xd., Gan, Zh. et al. Preparation of PLGA microspheres with different porous morphologies. Chin J Polym Sci 33, 128–136 (2015). https://doi.org/10.1007/s10118-014-1507-9

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  • DOI: https://doi.org/10.1007/s10118-014-1507-9

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