Cell and Tissue Research

, Volume 344, Issue 2, pp 217–225

Evidence for neural progenitor cells in the human adult enteric nervous system

  • Gaetano Azan
  • Walter C. Low
  • Gwen Wendelschafer-Crabb
  • Sayeed Ikramuddin
  • William R. Kennedy
Regular Article

DOI: 10.1007/s00441-011-1130-9

Cite this article as:
Azan, G., Low, W.C., Wendelschafer-Crabb, G. et al. Cell Tissue Res (2011) 344: 217. doi:10.1007/s00441-011-1130-9

Abstract

Putative neural stem cells have been identified within the enteric nervous system (ENS) of adult rodents and cultured from human myenteric plexus. We conducted studies to identify neural stem cells or progenitor cells within the submucosa of adult human ENS. Jejunum tissue was removed from adult human subjects undergoing gastric bypass surgery. The tissue was immunostained, and confocal images of ganglia in the submucosal plexus were collected to identify protein gene product 9.5 (PGP 9.5) - immunoractive neurons and neuronal progenitor cells that coexpress PGP 9.5 and nestin. In addition to PGP-9.5-positive/nestin-negative neuronal cells within ganglia, we observed two other types of cells: (1) cells in which PGP 9.5 and nestin were co-localized, (2) cells negative for both PGP 9.5 and nestin. These observations suggest that the latter two types of cells are related to a progenitor cell population and are consistent with the concept that the submucosa of human adult ENS contains stem cells capable of maintenance and repair within the peripheral nervous system.

Keywords

Enteric nervous systemNeural crestProtein gene product 9.5NestinNeural progenitor cellsHuman

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Gaetano Azan
    • 1
  • Walter C. Low
    • 3
    • 4
    • 5
  • Gwen Wendelschafer-Crabb
    • 1
  • Sayeed Ikramuddin
    • 2
  • William R. Kennedy
    • 1
  1. 1.Department of NeurologyUniversity of Minnesota Medical SchoolMinneapolisUSA
  2. 2.Department of SurgeryUniversity of Minnesota Medical SchoolMinneapolisUSA
  3. 3.Department of NeurosurgeryUniversity of Minnesota Medical SchoolMinneapolisUSA
  4. 4.Graduate Program in NeuroscienceUniversity of Minnesota Medical SchoolMinneapolisUSA
  5. 5.Stem Cell InstituteUniversity of Minnesota Medical SchoolMinneapolisUSA