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Strigolactones affect tomato hormone profile and somatic embryogenesis

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Abstract

Main conclusions

Exogenously applied GR24 affected somatic embryo formation and morphogenesis of strigolactone-deficient tomato mutant through cross-talk with auxins and cytokinins indicating involvement of SLs in the embryogenic process.

Strigolactones (SLs) mediate the regulation of plant responses to the environment through cross-talk with other plant hormones, especially auxins. Auxins play a crucial role in coordinating the morphogenesis and development of plant reproductive organs, including the signal-transduction cascade leading to the reprogramming of gene-expression patterns before embryo formation. SLs’ role in these processes is unknown, in contrast to their proven involvement in auxin transport and distribution. We used tomato cv. M82 and its SL-deficient mutant SL-ORT1 to study the influence of SLs on hormone profile in tomato roots and shoots, and their involvement in somatic embryogenesis (SE) and morphogenesis (adventitious root formation). The synthetic SL GR24 had different effects on SE of M82 and SL-ORT1, indicating that SLs influence the cytokinin-to-auxin ratio in tomato SE.

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Abbreviations

6-BA:

6-Benzylaminopurine

BM:

Basal medium

2,4-D:

2,4-Dichlorophenoxyacetic acid

IAA:

Indolelacetic acid

MS:

Murashige and Skoog

SL:

Strigolactone

SE:

Somatic embryogenesis

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Acknowledgements

We thank the Ministry of Finance of the State of Israel for financial support of Dr. Yuanli Wu’s post doctoral fellowship at Newe Ya’ar Research Center, Israel, and Prof. Koichi Yoneyama for kindly providing us with GR24.

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Authors and Affiliations

  1. Department of Phytopathology and Weed Research, Agricultural Research Organization (ARO), Newe Ya’ar Research Center, P.O. Box 1021, 30095, Ramat Yishay, Israel

    Yuanli Wu, Evgenia Dor & Joseph Hershenhorn

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  1. Yuanli Wu
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  2. Evgenia Dor
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  3. Joseph Hershenhorn
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Correspondence to Evgenia Dor.

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Wu, Y., Dor, E. & Hershenhorn, J. Strigolactones affect tomato hormone profile and somatic embryogenesis. Planta 245, 583–594 (2017). https://doi.org/10.1007/s00425-016-2625-0

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