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Topochemical and morphological characterization of wood cell wall treated with the ionic liquid, 1-ethylpyridinium bromide

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Abstract

Main conclusion

[EtPy][Br] is more reactive toward lignin than toward the PSs in wood cell walls, and [EtPy][Br] treatment results in inhomogenous changes to the cell wall’s ultrastructural and chemical components.

The effects of the ionic liquid 1-ethylpyridinium bromide ([EtPy][Br]), which prefers to react with lignin rather than cellulose on the wood cell walls of Japanese cedar (Cryptomeria japonica), were investigated from a morphology and topochemistry point of view. The [EtPy][Br] treatment induced cell wall swelling, the elimination of warts, and the formation of countless pores in the tracheids. However, many of the pit membranes and the cellulose crystalline structure remained unchanged. Raman microscopic analyses revealed that chemical changes in the cell walls were different for different layers and that the lignin in the compound middle lamella and the cell corner resists interaction with [EtPy][Br]. Additionally, the interaction of [EtPy][Br] with the wood cell wall is different to that of other types of ionic liquid.

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Abbreviations

CC:

Cell corner

CML:

Compound middle lamella

[C2mim][Cl]:

1-Ethyl-3-methylimidazolium chloride

[EtPy][Br]:

1-Ethylpyridinium bromide

PSs:

Polysaccharides

S2 :

Middle layer of secondary wall

S3 :

Inner layer of secondary wall

SEM:

Scanning electron microscopy

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Acknowledgments

The authors wish to thank the Kyoto Municipal Institute of Industrial Technology and Culture for assistance with the Raman microscopic analyses. This research was partly supported by the Kyoto Prefectural University (KPU) Academic Promotion Fund and a Grant-in-Aid for Scientific Research (c) (25450246) from the JSPS for which the authors are grateful.

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Authors and Affiliations

  1. Division of Environmental Sciences, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Hangi-cho, Shimogamo, Sakyo-ku, Kyoto, 606-8522, Japan

    Toru Kanbayashi & Hisashi Miyafuji

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  1. Toru Kanbayashi
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  2. Hisashi Miyafuji
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Correspondence to Hisashi Miyafuji.

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Special topic: Polyphenols: biosynthesis and function in plants and ecosystems. Guest editor: Stefan Martens.

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Kanbayashi, T., Miyafuji, H. Topochemical and morphological characterization of wood cell wall treated with the ionic liquid, 1-ethylpyridinium bromide. Planta 242, 509–518 (2015). https://doi.org/10.1007/s00425-014-2235-7

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