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Cellulosic biosensor chips for monitoring adsorptive interaction of rubisco protein with cellulose using SPR

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Abstract

Cellulosic films were prepared by spin-coating a nano-layer of cellulose from an organic solvent onto gold substrates. The cellulose-coated substrates were characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM) and surface plasmon resonance (SPR). Rubisco protein adsorptive interaction with the spin-coated cellulosic surfaces was monitored using SPR. The results have demonstrated that rubisco uptake by the spin-coated cellulosic films is minimal relative to other non-cellulosic films. The results have also revealed that rubisco uptake by the spin-coated cellulosic films is inconsistent, most likely due to the interference of the SPR reflectivity signal (i.e. measurement accuracy) by the swelling of the thick (~30 nm on dry basis) films. This inconsistency problem was resolved by preparing ultrathin films (~2.5 nm) of cellulose via the self-assembly of cellulose onto the SPR biosensor chips. The self-assembly was achieved by attaching disulphide functional group to the cellulose molecules through the chemical coupling with α-lipoic acid.

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Acknowledgements

The author acknowledges the financial support from the Newcastle University.

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  1. School of Chemical Engineering and Advanced Materials, Newcastle University, 535 Clementi Road, Blk 35 #02-01, Singapore, 599489, Singapore

    Sagheer A. Onaizi

  2. School of Chemical Engineering and Advanced Materials, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK

    Sagheer A. Onaizi

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Onaizi, S.A. Cellulosic biosensor chips for monitoring adsorptive interaction of rubisco protein with cellulose using SPR. Colloid Polym Sci 295, 849–857 (2017). https://doi.org/10.1007/s00396-017-4031-y

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