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Effect of light and auxin transport inhibitors on endoreduplication in hypocotyl and cotyledon

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Abstract

Key message

Enhancement of endoreduplication in dark-grown hypocotyl is a common feature in dicotyledonous polysomatic plants, and TIBA-mediated inhibition of the endoreduplication is partially due to abnormal actin organization.

Abstract

Many higher plant species use endoreduplication during cell differentiation. However, the mechanisms underlying this process have remained elusive. In this study, we examined endoreduplication in hypocotyls and cotyledons in response to light in some dicotyledonous plant species. Enhancement of endoreduplication was found in the dark-grown hypocotyls of all the polysomatic species analyzed across five different families, indicating that this process is a common feature in dicotyledonous plants having polysomatic tissues. Conversely, endoreduplication was enhanced in the light-grown cotyledons in four of the five species analyzed. We also analyzed the effect of a polar auxin transport inhibitor, 2,3,5-triiodobenzoic acid (TIBA) on endoreduplication in hypocotyl and cotyledon tissues of radish (Raphanus sativus L. var. longipinnatus Bailey). TIBA was found to inhibit and promote endoreduplication in hypocotyls and cotyledons, respectively, suggesting that the endoreduplication mechanism differs in these organs. To gain insight into the effect of TIBA, radish and spinach (Spinacia oleracea L.) seedlings were treated with a vesicle-trafficking inhibitor, brefeldin A, and an actin polymerization inhibitor, cytochalasin D. Both of the inhibitors partially inhibited endoreduplication of the dark-grown hypocotyl tissues, suggesting that the prominent inhibition of endoreduplication by TIBA might be attributed to its multifaceted role.

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Abbreviations

BFA:

Brefeldin A

CytD:

Cytochalasin D

DAG:

Days after germination

DAPI:

4′,6-diamidino-2-phenylindole

FCM:

Flow cytometry

HFCA:

9-hydroxyfluorene-9-carboxylic acid

NPA:

N-1-Naphthylphthalamic acid

PCIB:

2-(p-chlorophenoxy)-2-methylpropionic acid

TIBA:

2,3,5-Triiodobenzoic acid

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Acknowledgments

We would like to thank Mr. Junich Iijima (Cytotechs) and Dr. Kyoko Kano (Sysmex) for help with the flow cytometry. We also thank Editage (http://www.editage.jp) and Dr. Megumi Mishiba for English language editing.

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Authors and Affiliations

  1. Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen, Nakaku, Sakai, Osaka, 599-8531, Japan

    Riko Tanaka, Makoto Amijima, Yuji Iwata, Nozomu Koizumi & Kei-ichiro Mishiba

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  1. Riko Tanaka
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  2. Makoto Amijima
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  3. Yuji Iwata
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  4. Nozomu Koizumi
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  5. Kei-ichiro Mishiba
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Correspondence to Kei-ichiro Mishiba.

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Communicated by K. K. Kamo.

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Tanaka, R., Amijima, M., Iwata, Y. et al. Effect of light and auxin transport inhibitors on endoreduplication in hypocotyl and cotyledon. Plant Cell Rep 35, 2539–2547 (2016). https://doi.org/10.1007/s00299-016-2054-3

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  • DOI: https://doi.org/10.1007/s00299-016-2054-3

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