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The Populus trichocarpa PtHSP17.8 involved in heat and salt stress tolerances

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PtHSP17.8 was regulated by various abiotic stresses. Overexpression of PtHSP17.8 enhanced the tolerance to heat and salt stresses through maintain ROS homeostasis and cooperate with stress-related genes in Arabidopsis.

Abstract

Small heat shock proteins (sHSPs) play important roles in response to diverse biotic and abiotic stresses, especially in heat tolerance. However, limited information is available on the stress tolerance roles of sHSPs in woody species. To explore the function of sHSPs in poplar, we isolated and characterized PtHSP17.8 from Populus trichocarpa. Phylogenetic analysis and subcellular localization revealed that PtHSP17.8 was a cytosolic class I sHSP. The gene expression profile of PtHSP17.8 in various tissues showed that it was significantly accumulated in stem and root, which was consistent with the GUS expression pattern driven by promoter of PtHSP17.8. The expression of PtHSP17.8 could be induced by various abiotic stresses and significantly activated by heat stress. Overexpression of PtHSP17.8 enhanced the tolerance to heat and salt stresses in Arabidopsis. The seedling survival rate, root length, relative water content, antioxidative enzyme activities, proline, and soluble sugar content were increased in transgenic Arabidopsis under heat and salt stresses, but not in normal condition. The co-expression network of PtHSP17.8 were constructed and demonstrated many stress responsive genes included. The stress-related genes in the co-expression network were up-regulated in the PtHSP17.8 overexpression seedlings. These results suggest that PtHSP17.8 confers heat and salt tolerances in plants.

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Acknowledgments

This work was supported by the China Postdoctoral Science Foundation [2014 M550104] to J.Z. and the National Key Basic Research Program of China [2012CB114500] and a Collaborative Innovation Plan of Jiangsu Higher Education to M.L.

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    Authors and Affiliations

    1. State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China

      Jianbo Li, Jin Zhang, Huixia Jia, Yu Li, Xiangdong Xu, Lijuan Wang & Mengzhu Lu

    2. Collaborative Innovation Center of Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, 210037, China

      Jin Zhang, Huixia Jia & Mengzhu Lu

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    1. Jianbo Li
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    2. Jin Zhang
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    4. Yu Li
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    Correspondence to Jin Zhang or Mengzhu Lu.

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    Communicated by K Chong.

    J. Li and J. Zhang contributed equally to this work.

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    299_2016_1973_MOESM1_ESM.tif

    Fig. S1 The cis- acting elements in promoter of PtHSP17.8 gene were searched in PlantCARE database. The promoter region (1.5 kb upstream to 0.5 kb downstream of the transcription starting site) of PtHSP17.8 was analyzed in PlantCARE database (a). The description and statistics of cis-acting elements were shown in (b). (TIFF 1107 kb)

    Table S1. Primer sequences used in this study (XLSX 10 kb)

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    Li, J., Zhang, J., Jia, H. et al. The Populus trichocarpa PtHSP17.8 involved in heat and salt stress tolerances. Plant Cell Rep 35, 1587–1599 (2016). https://doi.org/10.1007/s00299-016-1973-3

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