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Identification, isolation and expression analysis of eight stress-related R2R3-MYB genes in tartary buckwheat (Fagopyrum tataricum)

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Eight R2R3 - MYB genes in tartary buckwheat were identified, and their expression patterns were comprehensively analyzed, which reveals role in plant response to abiotic stresses.

Abstract

The proteins of the R2R3-MYB superfamily play key roles in the growth and development processes as well as defense responses in plants. However, their characteristics and functions have not been fully investigated in tartary buckwheat (Fagopyrum tataricum), a strongly abiotic resistant coarse cereal. In this article, eight tartary buckwheat R2R3-MYB genes were isolated with full-length cDNA and DNA sequences. Phylogenetic analysis of the members of the R2R3-MYB superfamily between Arabidopsis and tartary buckwheat revealed that the assumed functions of the eight tartary buckwheat R2R3-MYB proteins are divided into five Arabidopsis functional subgroups that are involved in abiotic stress. Expression analysis during abiotic stress and exogenous phytohormone treatments identified that the eight R2R3-MYB genes responded to one or more treatments. This study is the first comprehensive analysis of the R2R3-MYB gene family in tartary buckwheat under abiotic stress.

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Acknowledgments

This work was supported by the Science and Technology Department of Sichuan Province, PR China (2015HH0047).

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Authors and Affiliations

  1. College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, 625014, Ya’an, Sichuan, China

    Fei Gao, Hai-Xia Zhao, Hui-Peng Yao, Cheng-Lei Li, Hui Chen & Qi Wu

  2. Xichang College, 615013, Xichang, Sichuan, China

    An-Hu Wang

  3. Department of Crop Science, College of Agriculture and Life Sciences, Chungnam National University, Gung-Dong, South Korea

    Sang-Un Park

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  1. Fei Gao
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Correspondence to Qi Wu.

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Communicated by K. Chong.

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Gao, F., Zhao, HX., Yao, HP. et al. Identification, isolation and expression analysis of eight stress-related R2R3-MYB genes in tartary buckwheat (Fagopyrum tataricum). Plant Cell Rep 35, 1385–1396 (2016). https://doi.org/10.1007/s00299-016-1971-5

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