Abstract
Key message
Protopanaxadiol (PPD) is an aglycone of dammarene-type ginsenoside and has high medicinal values. In this work, we reported the PPD production in transgenic tobacco co-overexpressing PgDDS and CYP716A47.
Abstract
PPD is an aglycone of ginsenosides produced by Panax species and has a wide range of pharmacological activities. PPD is synthesized via the hydroxylation of dammarenediol-II (DD) by CYP716A47 enzyme. Here, we established a PPD production system via cell suspension culture of transgenic tobacco co-overexpressing the genes for PgDDS and CYP716A47. The concentration of PPD in transgenic tobacco leaves was 2.3–5.7 µg/g dry weight (DW), depending on the transgenic line. Leaf segments were cultured on medium with various types of hormones to induce callus. Auxin treatment, particularly 2,4-D, strongly enhanced the production of DD (783.8 µg g−1 DW) and PPD (125.9 µg g−1 DW). Treatment with 2,4-D enhanced the transcription of the HMG-Co reductase (HMGR) and squalene epoxidase genes. PPD production reached 166.9 and 980.9 µg g−1 DW in a 250-ml shake flask culture and in 5-l airlift bioreactor culture, respectively.
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Abbreviations
- BAR:
-
Basta
- DD:
-
Dammarenediol-II
- PPD:
-
Protopanaxadiol
- DDS:
-
Dammarenediol-II synthase
- PgDDS:
-
Panax ginseng dammarenediol-II synthase
- CYP716A47:
-
Cytochrome P450 716A47
- qPCR:
-
Real-time polymerase chain reaction
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Acknowledgments
This work was supported by the Rural Development Administration, Republic of Korea [Next-Generation BioGreen 21 Program (PJ011285)], and by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2013R1A1A2064352).
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The authors declare that they have no conflict of interest.
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Communicated by F. Sato.
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Chun, JH., Adhikari, P.B., Park, SB. et al. Production of the dammarene sapogenin (protopanaxadiol) in transgenic tobacco plants and cultured cells by heterologous expression of PgDDS and CYP716A47 . Plant Cell Rep 34, 1551–1560 (2015). https://doi.org/10.1007/s00299-015-1806-9
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DOI: https://doi.org/10.1007/s00299-015-1806-9