Abstract
Key message
PGbEXPA2 (Promoter of GbEXPA2 ) was preferentially and strongly expressed during cotton fiber development, and the 461-bp PGbEXPA2 fragment was essential for responding to exogenous GA and ABA in Arabidopsis.
Abstract
Cotton fibers are highly elongated single-cell, unbranched and non-glandular seed trichomes. Previous studies have reported that the transcript level of GbEXPA2 is significantly up-regulated during fiber cell elongation, suggesting that GbEXPA2 has an important function in fiber development. In this study, the promoter of GbEXPA2 (839 bp) from the DT sub-genome was isolated from Gossypium barbadense 3–79. Consistent with the expression pattern of GbEXPA2, the promoter PGbEXPA2 was able to express GUS to high levels in elongating fibers, but not in the root, stem, or leaf. In Arabidopsis, GUS activity was only found in the rosette leaf trichomes and rosette leaf vascular tissue, indicating that the transcription factors which bind to PGbEXPA2 in the leaf trichomes of transgenic Arabidopsis were similar to those found in cotton fiber. A deletion analysis of PGbEXPA2 revealed that a 461-bp fragment was sufficient to drive GUS expression in cotton fibers and Arabidopsis rosette leaf trichomes. Exogenous phytohormonal treatments on transgenic Arabidopsis with different promoter lengths (P-839, P-705, P-588 and P-461) showed that GUS activity in Arabidopsis trichomes could be strongly up-regulated by GA and, in contrast, down-regulated by ABA.
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Abbreviations
- ABA:
-
Abscisic acid
- CaMV 35S:
-
Cauliflower mosaic virus 35S promoter
- DPA:
-
Days post anthesis
- EXPA:
-
α-expansin
- GA:
-
Gibberellins
- Gb :
-
Gossypium barbadense
- GUS:
-
Beta-glucuronidase
- NPTII:
-
Neomycin phosphotransferase
- PCR:
-
Polymerase chain reaction
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Acknowledgments
This project is supported by the National Natural Science Foundation of China (30871560) and GMO Crops Development Project from the Ministry of Agriculture (2013ZX08010001). We thank Sally Walford for her critical comments on the manuscript (CSIRO, Plant industry, Australia).
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Communicated by A. Feher.
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Li, Y., Tu, L., Ye, Z. et al. A cotton fiber-preferential promoter, PGbEXPA2, is regulated by GA and ABA in Arabidopsis . Plant Cell Rep 34, 1539–1549 (2015). https://doi.org/10.1007/s00299-015-1805-x
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DOI: https://doi.org/10.1007/s00299-015-1805-x