Skip to main content
SpringerLink
Log in

Cloning and expression analysis of an MYB gene associated with calyx persistence in Korla fragrant pear

  • Original Paper
  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Key message

We isolated an MYB-like gene from Korla fragrant pear using differential display RT-PCR. Expression of this gene in flowers appears to be correlated with calyx persistence.

Abstract

Korla fragrant pear (Pyrus brestschneideri Rehd) is an economically important pear cultivar in China. A persistent calyx results in the deformation of the fruit. We used differential display RT-PCR to obtain 42 cDNA fragments from Korla fragrant pear flowers. Alignments of nucleotide and amino acid sequences suggested that two fragments (kfp1and kfp4) were related to calyx persistence. The fragments were 78 % homologous with Malus × domestica SPL transcription factor (SPL3) and 83 % homologous with Malus × domestica MYB transcription factor (MYB12). The complete cDNA sequence of kfpMYB was determined to clarify the role of MYB in calyx persistence. kfpMYB contained a 116 bp 5′-UTR, a 1122 bp open reading frame encoding 374 amino acids, and a 319 bp 3′-UTR. The nucleotide and amino acid sequences of the cDNA in Korla fragrant pear were highly homologous with those of MYB transcription factors in other plant species, suggesting that the sequence is a MYB transcription factor gene. The abundance of kfpMYB mRNA varied significantly between the second and fourth flowers on the branch. Furthermore, kfpMYB expression changed significantly during anthesis and was significantly higher in Jinfeng pear (persistent calyx) and Korla fragrant pear than in Yali pear (deciduous calyx). Expression of kfpMYB was significantly reduced by naphthalene (NAA), abscisic acid (ABA), PBO, and paclobutrazol (PP333). Uniconazole, ethylene (ETH), and gibberellic acid (GA3) had no signicant effect on kfpMYB expression. In conclusion, the expression of kfpMYB appears to be correlated with calyx persistence in Korla fragrant pear.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

References

  • Abe H, Yamaguchi-Shinozaki K, Urao T, Iwasaki T, Hosokawa D, Shinozaki K (1997) Role of Arabidopsis MYC and MYB homologs in drought- and abscisic acid-regulated gene expression. Plant Cell 9:1859–1868

    CAS  PubMed Central  PubMed  Google Scholar 

  • Abe H, Urao T, Ito T, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2003) Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. Plant Cell 15:63–78

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Achard P, Herr A, Baulcombe DC, Harberd NP (2004) Modulation of floral development by a gibberellin-regulated microRNA. Development 131:3357–3365

    Article  CAS  PubMed  Google Scholar 

  • Baudry A, Heim MA, Dubreucq B, Caboche M, Weisshaar B, Lepiniec L (2004) TT2, TT8, and TTG1 synergistically specify the expression of BANYULS and proanthocyanidin biosynthesis in Arabidopsis thaliana. Plant J 39:366–380

    Article  CAS  PubMed  Google Scholar 

  • Borevitz JO, Xia YJ, Blount J, Dixon RA, Lamb C (2000) Activation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis. Plant Cell 12:2383–2393

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Cai XZ, Xu YP, Lou J (2003) Cloning of tomato MYB gene fragments and expression upon development of hypersensitive response. Acta Hort Sin 30:589–591

    Google Scholar 

  • Du H, Yang WJ, Liu L, Tang XF, Wu YM, Huang YB, Tang YX (2008) Cloning and functional identification of the Two MYB transcription factors GmMYBJ6 and GmMYBJ7 in soybean. Acta Agronomica Sinica 34:1179–1187

    CAS  Google Scholar 

  • Du H, Zhang L, Liu L, Tang XF, Yang WJ, Wu YM, Huang YB, Tang YX (2009) Biochemical and molecular characterization of plant MYB transcription factor family. Biochemistry 1:1–11

    Google Scholar 

  • Gocal GFW, Poole AT, Gubler F, Watts RJ, Blundell C, King RW (1999) Long-Day up-ragulation of a GAMYB gene during Lolium temulentum inflorescence formation. Plant Physiol 119:1271–1278

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • He M, Li HS, Zhao Y, Niu JX, Ma BG (2004) A method of rapidly extracting total rna from kurle aroma pear. Journal of Shihezi University (Natural Science) 22(6):274–276

    Google Scholar 

  • He ZS, Niu JX, Shao YX (2006) The study of calyx leaving and persistent of Korla fragrant pear. China Fruit Vegetable 2:10–11

    Google Scholar 

  • Higginson T, Li SF, Parish RW (2003) AtMYB103 regulates tapetum and trichome development in Arabidopsis thaliana. Plant J 35:177–192

    Article  CAS  PubMed  Google Scholar 

  • Ito M, Araki S, Matsunaga S, Itoh T, Nishihama R, Machida Y, Doonan JH, Watanabe A (2001) G2/M-phase-specific transcription during the plant cell cycle is mediated by c-MYB-like transcription factors. Plant Cell 13:1891–1905

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Jin HL, Cominelli E, Bailey P, Parr A, Mehrtens F, Jones J, Tonelli C, Weisshaar B, Martin C (2000) Transcriptional repression by AtMYB4 controls production of UV-protecting sunscreens in Arabidopsis. EMBO J 19:6150–6161

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Jing CZ, Wang YD (2002) The preliminary study on rootstocks of Korla fragrant pear. Northern Fruits 1:13

    Google Scholar 

  • Kaneko M, Inukai Y, Ueguchi-Tanaka M, Itoh H, Izawa T, Kobayashi Y, Hattori T, Miyao A, Hirochika H, Ashikari M, Matsuoka M (2004) Loss-of-function mutations of the rice GAMYB gene impair α-amylase expression in aleurone and flower development. Plant Cell 16:33–44

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Lee MM, Schiefelbein J (2002) Cell pattern in the Arabidopsis root epidermis determined by lateral inhibition with feedback. Plant Cell 14:611–618

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Li L, He XX (2008) The study of plant growth regulators on controlling calyx leaving and persistent from fruit of Korla fragrant pear. Forestry of Xinjiang 1:29–30

    Google Scholar 

  • Liang P, Pardee AB (1992) Differential display of eukaryotic messenger RNA by means of polymerase chain reaction. Science 257:967–971

    Article  CAS  PubMed  Google Scholar 

  • Mei LZ, Chen L, Zhou JM (1995) The influence of growth and fruiting of PP333 on young trees of Korla fragrant pear.China. Fruits 2:20–21

    Google Scholar 

  • Murray F, Kalla R, Jacobsen J, Gubler F (2003) A role for HvGAMYB in anther development. Plant J 33:481–491

    Article  CAS  PubMed  Google Scholar 

  • Nesi N, Jond C, Debeaujon I, Caboche M, Lepiniec L (2001) The Arabidopsis TT2 gene encodes an R2R3 MYB domain protein that acts as a key determainant for proanthocyanidin accumulation in developing seed. Plant Cell 13:2099–2114

    CAS  PubMed Central  PubMed  Google Scholar 

  • Palatnik JF, Wollmann H, Schommer C, Schwab R, Boisbouvier J, Rodriguez R, Warthmann N, Allen E, Dezulian T, Huson D, Carrington JC, Weigel D (2007) Sequence and expression differences underlie functional specialization of Arabidopsis microRNAs miR159 and miR319. Dev Cell 13:115–125

    Article  CAS  PubMed  Google Scholar 

  • Penfield S, Meissner RC, Shoue DA, Carpita NC, Bevan MW (2001) MYB61 is required for mucilage deposition and extrusion in the Arabidopsis seed coat. Plant Cell 13:2777–2791

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Raffaele S, Rivas S, Roby D (2006) An essential role for salicylic acid in AtMYB30-mediated control of the hypersensitive cell death program in Arabidopsis. FEBS Lett 580:3498–3504

    Article  CAS  PubMed  Google Scholar 

  • Ren YY, Li J, Qin WM, Guo QZ (2007) Preliminary studies on characteristic and control of calyx leaving and persistent from fruit of Korla fragrant pear. Journal of Xinjiang Agricultural University 30:25–29

    CAS  Google Scholar 

  • Ruan X, Wang Q, Zhou MJ, Zhen CX (2001) The relationship between endogenous hormone of fragrant pear fruit and Ca-Lyx-end tubercle, abscission of fruit and schizocarpic fruit. Plant Physiology Communications 37:220–221

    CAS  Google Scholar 

  • Schmitz G, Tillmann E, Carriero F, Fiore C, Cellini F, Theres K (2002) The tomato Blind gene encodes a MYB transcription factor that controls the formation of lateral meristems. PNAS 99:1064–1069

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Shao YX, Niu JX, He ZS (2007) The study on calyx leaving and persisten of Korla fragrant pear. Modern Agricultural Sciences and Technology 10:46–49

    Google Scholar 

  • Shin B, Choi G, Yi H, Yang S, Cho I, Kim J, Lee S, Paek NC, Kim JH, Song PS, Choi G (2002) AtMYB21, a gene encoding a flower-specific transcription factor, is regulated by COP1. Plant J 30:23–32

    Article  CAS  PubMed  Google Scholar 

  • Steiner-Lange S, Unte US, Eckstein L, Yang CY, Wilson ZA, Schmelzer E, Dekker K, Saedler H (2003) Disruption of Arabidopsis thaliana MYB26 results in male sterility due to non-dehiscent anthers. Plant J 34:519–528

    Article  CAS  PubMed  Google Scholar 

  • Xu QY (1997) The influence of fruit characteristic of pollination conditions on Korla fragrant pear. Journal of Fruit Science 14:113–114

    Google Scholar 

  • Yuan JH (2010) Experimental research on real-time fluorescent quantitative PCR. Modern Agricultural Sciences and Technology 13:20–22

    Google Scholar 

  • Zhu JH, Verslues PE, Zheng XW, Lee BH, Zhan XQ, Manabe Y, Sokolchik I, Zhu YM, Dong CH, Zhu JK, Hasegawa PM, Bressan RA (2005) HOS10 encodes an R2R3-type MYB transcription factor essential for cold acclimation in plants. PNAS 102:9966–9971

    Article  CAS  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (31360474), the National Research Program to Guide the Project (2003BA546C), and the International Scientific and Technological Cooperation Projects (2009YD32).

Author information

Authors and Affiliations

  1. Department of Horticulture, College of Agriculture, Shihezi University, 832003, Shihezi, People’s Republic of China

    Bo-Hui Wang, Xiao-Xia Sun, Fang-Yuan Dong, Fei Zhang & Jian-Xin Niu

Authors
  1. Bo-Hui Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiao-Xia Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fang-Yuan Dong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Fei Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jian-Xin Niu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jian-Xin Niu.

Additional information

Communicated by Z.-Y. Wang.

Bo-Hui Wang, Xiao-Xia Sun, Fang-Yuan Dong and Fei Zhang are equally contributed to the work.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wang, BH., Sun, XX., Dong, FY. et al. Cloning and expression analysis of an MYB gene associated with calyx persistence in Korla fragrant pear. Plant Cell Rep 33, 1333–1341 (2014). https://doi.org/10.1007/s00299-014-1619-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00299-014-1619-2

Keywords

Search

Navigation