Plant Cell Reports

, Volume 25, Issue 11, pp 1133–1137

Hormone-regulated inflorescence induction and TFL1 expression in Arabidopsis callus in vitro

Cell Biology and Morphogenesis

DOI: 10.1007/s00299-006-0165-y

Cite this article as:
Guan, C.M., Zhu, S.S., Li, X.G. et al. Plant Cell Rep (2006) 25: 1133. doi:10.1007/s00299-006-0165-y

Abstract

To study hormone-regulated inflorescence development, we established the in vitro regeneration system of Arabidopsis inflorescences in the presence of cytokinin and auxin. Media containing a combination of thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D) were used to induce callus formation. Higher frequencies of calli were obtained by using the inflorescence stems as explants. After transferring the calli to media containing a combination of zeatin and indole-3-acetic acid (IAA), the inflorescences were induced from the calli. The morphology of regenerated inflorescences was similar to that of inflorescences in plants; however, flowers of regenerated inflorescences often lacked a few floral organs. Furthermore, TFL1, a gene involved in floral transition in Arabidopsis, was activated during the inflorescence induction. Our results suggest that the TFL1 gene plays an important role in hormone-regulated inflorescence formation.

Keywords

Arabidopsis thalianaCallusHormonesInflorescence induction

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • C. M. Guan
    • 1
  • S. S. Zhu
    • 1
  • X. G. Li
    • 1
  • X. S. Zhang
    • 1
  1. 1.Shandong Key Laboratory of Crop Biology, College of Life SciencesShandong Agricultural UniversityTaianChina