Abstract
To study hormone-regulated inflorescence development, we established the in vitro regeneration system of Arabidopsis inflorescences in the presence of cytokinin and auxin. Media containing a combination of thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D) were used to induce callus formation. Higher frequencies of calli were obtained by using the inflorescence stems as explants. After transferring the calli to media containing a combination of zeatin and indole-3-acetic acid (IAA), the inflorescences were induced from the calli. The morphology of regenerated inflorescences was similar to that of inflorescences in plants; however, flowers of regenerated inflorescences often lacked a few floral organs. Furthermore, TFL1, a gene involved in floral transition in Arabidopsis, was activated during the inflorescence induction. Our results suggest that the TFL1 gene plays an important role in hormone-regulated inflorescence formation.
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X.S. Zhang was supported by the National Natural Science Foundation of China (30370143) for this research.
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Guan, C.M., Zhu, S.S., Li, X.G. et al. Hormone-regulated inflorescence induction and TFL1 expression in Arabidopsis callus in vitro. Plant Cell Rep 25, 1133–1137 (2006). https://doi.org/10.1007/s00299-006-0165-y
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DOI: https://doi.org/10.1007/s00299-006-0165-y