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Preparation of gold nanoparticles/single-walled carbon nanotubes/polyaniline composite-coated electrode developed for DNA detection

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Abstract

This work details the preparation of gold nanoparticles/single-walled carbon nanotubes/polyaniline composite-modified disposable pencil graphite electrode (AuNPs/SWCNTs/PANI/PGEs), and its application for electrochemical DNA sensing. Disposable pencil graphite electrode was modified with single-walled carbon nanotubes/polyaniline composite via one-step electropolymerization of aniline solution containing well-dispersed single-walled carbon nanotubes. AuNPs were then immobilized onto the SWCNTs/PANI/PGE. Electrochemical results exhibited improved sensitivity for DNA detection with AuNPs/SWCNTs/PANI/PGE compared to the SWCNTs/PANI/PGE, PANI/PGE or unmodified PGE due to the strong interaction between AuNPs, SWCNTs, and PANI based on the effective degree of electron delocalization. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to characterize the prepared electrode. Thiol-linked ssDNA was immobilized onto the AuNPs/SWCNTs/PANI/PGE for DNA detection. Experimental parameters such as the concentration of AuNPs and ssDNA were investigated to optimize the experimental conditions.

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    Authors and Affiliations

    1. Analytical Chemistry Department, Faculty of Pharmacy, Ege University, Bornova, 35100, Izmir, Turkey

      Ece Eksin & Arzum Erdem

    2. Department of Chemistry, Faculty of Science, Hacettepe University, Beytepe, 06800, Ankara, Turkey

      Gulcin Bolat & Serdar Abaci

    3. Department of Chemistry, Faculty of Arts and Sciences, Ordu University, 52200, Ordu, Turkey

      Filiz Kuralay

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    1. Ece Eksin
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    2. Gulcin Bolat
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    3. Filiz Kuralay
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    4. Arzum Erdem
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    5. Serdar Abaci
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    Correspondence to Arzum Erdem or Serdar Abaci.

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    All authors certify that there is no conflict of interest and the submission is original work and is not under review in any other journal. All co-authors have seen and agree with the contents of the manuscript and there is no financial interest to report.

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    E. Eksin and G. Bolat contributed equally to this work.

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    Eksin, E., Bolat, G., Kuralay, F. et al. Preparation of gold nanoparticles/single-walled carbon nanotubes/polyaniline composite-coated electrode developed for DNA detection. Polym. Bull. 72, 3135–3146 (2015). https://doi.org/10.1007/s00289-015-1457-6

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    • DOI: https://doi.org/10.1007/s00289-015-1457-6

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