Abstract
Purpose
Increasing evidence suggests that alterations in mitochondrial DNA (mtDNA) copy number (mtDNAcn) and relative telomere length (RTL) may be implicated in the tumorigenesis of several malignancies. Alterations of both RTL and mtDNAcn are generally accepted as independent biomarkers for predicting risk and prognosis in various cancers. The aim of this study was to evaluate the prognostic value of combining leukocyte RTL with mtDNAcn (RTL–mtDNAcn) in hepatocellular carcinoma (HCC).
Methods
RTL and mtDNAcn in peripheral blood leukocytes (PBLs) were measured using a real-time PCR-based method in a total of 250 HCC patients treated with transcatheter arterial chemoembolization (TACE). We evaluated the associations between RTL and/or mtDNAcn and HCC overall survival using Kaplan–Meier curve analysis and Cox proportional hazards regression model.
Results
We found that patients with longer leukocyte RTL or lower mtDNAcn had shorter overall survival time. The univariate analysis (HR 1.63, 95 % CI 1.23–2.17, P = 7.7 × 10−4) and multivariate analysis (HR 1.78, 95 % CI 1.31–2.42, P = 2.4 × 10−4) indicated that longer leukocyte RTL was significantly associated with poorer OS in HCC patients. Kaplan–Meier curve analysis showed that patients with longer RTL had shorter overall survival time than those with shorter RTL (log-rank P = 0.001). Patients with lower mtDNA copy number was significantly associated with poorer OS by Cox proportional hazards model using both univariate (HR 1.60, 95 % CI 1.21–2.13, P = 0.001) and multivariate analyses (HR 1.77, 95 % CI 1.30–2.41, P = 2.8 × 10−4). Kaplan–Meier curve analysis showed that patients with lower mtDNA content had significantly shorter overall survival time than those with higher mtDNA content (log-rank P = 0.001). Furthermore, combination of leukocyte RTL and mtDNAcn significantly improved the efficacy of predicting HCC prognosis. Patients with longer RTL and lower mtDNAcn exhibited a significantly poorer overall survival in both the univariate analysis (HR 2.21, 95 % CI 1.52–3.22, P = 3.5 × 10−5) and multivariate analysis (HR 2.60, 95 % CI 1.73–3.90, P = 4.3 × 10−6). The effect on patient prognosis was more evident in patients with longer RTL and lower mtDNAcn than in those with shorter RTL and lower mtDNA (HR 2.11, 95 % CI 1.34–3.32, P = 0.001) or in those with longer RTL and higher mtDNA (HR 2.10, 95 % CI 1.34–3.27, P = 0.001).
Conclusions
Our data suggest that combination of leukocyte RTL-mtDNAcn may be a potential efficient prognostic marker for HCC patients receiving the TACE treatment.
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References
Siegel RL, Miller KD, Jemal A (2016) Cancer statistics, 2016. CA Cancer J Clin 66(1):7–30. doi:10.3322/caac.21332
Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, Jemal A, Yu XQ, He J (2016) Cancer statistics in China, 2015. CA cancer J clin. doi:10.3322/caac.21338
Yeh JH, Hung CH, Wang JH, Chen CH, Kee KM, Kuo CM, Yen YH, Cheng YF, Chen YY, Hsu HC, Lu SN (2015) Modifiable prognostic factors of hepatocellular carcinoma in patients with non-surgical treatment. PLoS One 10(12):e0144893. doi:10.1371/journal.pone.0144893
Yu X, Ge N, Guo X, Shen S, Liang J, Huang X, Wan S, Xing J, Huang Q, Yang Y (2014) Genetic variants in the EPCAM gene is associated with the prognosis of transarterial chemoembolization treated hepatocellular carcinoma with portal vein tumor thrombus. PLoS One 9(4):e93416. doi:10.1371/journal.pone.0093416
Bisoffi M, Heaphy CM, Griffith JK (2006) Telomeres: prognostic markers for solid tumors. Int J Cancer 119(10):2255–2260. doi:10.1002/ijc.22120
Sun B, Wang Y, Kota K, Shi Y, Motlak S, Makambi K, Loffredo CA, Shields PG, Yang Q, Harris CC, Zheng YL (2015) Telomere length variation: a potential new telomere biomarker for lung cancer risk. Lung Cancer 88(3):297–303. doi:10.1016/j.lungcan.2015.03.011
Svenson U, Nordfjall K, Stegmayr B, Manjer J, Nilsson P, Tavelin B, Henriksson R, Lenner P, Roos G (2008) Breast cancer survival is associated with telomere length in peripheral blood cells. Cancer Res 68(10):3618–3623. doi:10.1158/0008-5472.CAN-07-6497
Fu X, Wan S, Hann HW, Myers RE, Hann RS, Au J, Chen B, Xing J, Yang H (2012) Relative telomere length: a novel non-invasive biomarker for the risk of non-cirrhotic hepatocellular carcinoma in patients with chronic hepatitis B infection. Eur J Cancer 48(7):1014–1022. doi:10.1016/j.ejca.2012.02.066
Svenson U, Ljungberg B, Roos G (2009) Telomere length in peripheral blood predicts survival in clear cell renal cell carcinoma. Cancer Res 69(7):2896–2901. doi:10.1158/0008-5472.CAN-08-3513
Liu HQ, An JZ, Liu J, Yang YF, Zhang HX, Zhao BY, Li JB, Yang HS, Chen ZN, Xing JL (2012) Leukocyte telomere length predicts overall survival in hepatocellular carcinoma treated with transarterial chemoembolization. Carcinogenesis 33(5):1040–1045. doi:10.1093/carcin/bgs098
Chen Y, Qu F, He X, Bao G, Liu X, Wan S, Xing J (2014) Short leukocyte telomere length predicts poor prognosis and indicates altered immune functions in colorectal cancer patients. Ann Oncol : official J Eur Soc Med Oncology/ESMO 25(4):869–876. doi:10.1093/annonc/mdu016
Oh BK, Kim H, Park YN, Yoo JE, Choi J, Kim KS, Lee JJ, Park C (2008) High telomerase activity and long telomeres in advanced hepatocellular carcinomas with poor prognosis. Lab Invest 88(2):144–152. doi:10.1038/labinvest.3700710
Chan DC (2006) Mitochondria: dynamic organelles in disease, aging, and development. Cell 125(7):1241–1252. doi:10.1016/j.cell.2006.06.010
Shadel GS (2008) Expression and maintenance of mitochondrial DNA: new insights into human disease pathology. Am J Pathol 172(6):1445–1456. doi:10.2353/ajpath.2008.071163
Wang Y, Liu VW, Xue WC, Tsang PC, Cheung AN, Ngan HY (2005) The increase of mitochondrial DNA content in endometrial adenocarcinoma cells: a quantitative study using laser-captured microdissected tissues. Gynecol Oncol 98(1):104–110. doi:10.1016/j.ygyno.2005.04.015
Wang Y, Liu VW, Xue WC, Cheung AN, Ngan HY (2006) Association of decreased mitochondrial DNA content with ovarian cancer progression. Br J Cancer 95(8):1087–1091. doi:10.1038/sj.bjc.6603377
Qu F, Liu X, Zhou F, Yang H, Bao G, He X, Xing J (2011) Association between mitochondrial DNA content in leukocytes and colorectal cancer risk: a case-control analysis. Cancer 117(14):3148–3155. doi:10.1002/cncr.25906
Lemnrau A, Brook MN, Fletcher O, Coulson P, Tomczyk K, Jones M, Ashworth A, Swerdlow A, Orr N, Garcia-Closas M (2015) Mitochondrial DNA copy number in peripheral blood cells and risk of developing breast cancer. Cancer Res 75(14):2844–2850. doi:10.1158/0008-5472.CAN-14-1692
Xing J, Chen M, Wood CG, Lin J, Spitz MR, Ma J, Amos CI, Shields PG, Benowitz NL, Gu J, de Andrade M, Swan GE, Wu X (2008) Mitochondrial DNA content: its genetic heritability and association with renal cell carcinoma. J Natl Cancer Inst 100(15):1104–1112. doi:10.1093/jnci/djn213
Hosgood HD 3rd, Liu CS, Rothman N, Weinstein SJ, Bonner MR, Shen M, Lim U, Virtamo J, Cheng WL, Albanes D, Lan Q (2010) Mitochondrial DNA copy number and lung cancer risk in a prospective cohort study. Carcinogenesis 31(5):847–849. doi:10.1093/carcin/bgq045
Zhang J, Li D, Qu F, Chen Y, Li G, Jiang H, Huang X, Yang H, Xing J (2014) Association of leukocyte mitochondrial DNA content with glioma risk: evidence from a Chinese case-control study. BMC Cancer 14:680. doi:10.1186/1471-2407-14-680
Lan Q, Lim U, Liu CS, Weinstein SJ, Chanock S, Bonner MR, Virtamo J, Albanes D, Rothman N (2008) A prospective study of mitochondrial DNA copy number and risk of non-Hodgkin lymphoma. Blood 112(10):4247–4249. doi:10.1182/blood-2008-05-157974
Yuan P, Wang S, Zhou F, Wan S, Yang Y, Huang X, Zhang Z, Zhu Y, Zhang H, Xing J (2014) Functional polymorphisms in the NPAS2 gene are associated with overall survival in transcatheter arterial chemoembolization-treated hepatocellular carcinoma patients. Cancer Sci 105(7):825–832. doi:10.1111/cas.12428
Cawthon RM (2002) Telomere measurement by quantitative PCR. Nucleic Acids Res 30(10):e47
Liu J, Yang Y, Zhang H, Zhao S, Liu H, Ge N, Yang H, Xing JL, Chen Z (2011) Longer leukocyte telomere length predicts increased risk of hepatitis B virus-related hepatocellular carcinoma: a case-control analysis. Cancer 117(18):4247–4256. doi:10.1002/cncr.26015
Zhang C, Chen X, Li L, Zhou Y, Wang C, Hou S (2015) The association between telomere length and cancer prognosis: evidence from a meta-analysis. PLoS One 10(7):e0133174. doi:10.1371/journal.pone.0133174
Svenson U, Roos G (2009) Telomere length as a biological marker in malignancy. Biochim Biophys Acta 1792(4):317–323. doi:10.1016/j.bbadis.2009.01.017
Qu F, Li R, He X, Li Q, Xie S, Gong L, Ji G, Lu J, Bao G (2015) Short telomere length in peripheral blood leukocyte predicts poor prognosis and indicates an immunosuppressive phenotype in gastric cancer patients. Mol Oncol 9(3):727–739. doi:10.1016/j.molonc.2014.11.008
Lin CS, Chang SC, Wang LS, Chou TY, Hsu WH, Wu YC, Wei YH (2010) The role of mitochondrial DNA alterations in esophageal squamous cell carcinomas. J thorac cardiovasc surg 139(1):189–197. doi:10.1016/j.jtcvs.2009.04.007
Guo W, Yang D, Xu H, Zhang Y, Huang J, Yang Z, Chen X, Huang Z (2013) Mutations in the D-loop region and increased copy number of mitochondrial DNA in human laryngeal squamous cell carcinoma. Mol Biol Rep 40(1):13–20. doi:10.1007/s11033-012-1939-7
Cheau-Feng Lin F, Jeng YC, Huang TY, Chi CS, Chou MC, Chin-Shaw Tsai S (2014) Mitochondrial DNA copy number is associated with diagnosis and prognosis of head and neck cancer. Biomarkers 19(4):269–274. doi:10.3109/1354750X.2014.902101
Bekaert S, De Meyer T, Van Oostveldt P (2005) Telomere attrition as ageing biomarker. Anticancer Res 25(4):3011–3021
Tyrka AR, Parade SH, Price LH, Kao HT, Porton B, Philip NS, Welch ES, Carpenter LL (2016) Alterations of mitochondrial DNA copy number and telomere length with early adversity and psychopathology. Biol Psychiatry 79(2):78–86. doi:10.1016/j.biopsych.2014.12.025
Codd V, Mangino M, van der Harst P, Braund PS, Kaiser M, Beveridge AJ, Rafelt S, Moore J, Nelson C, Soranzo N, Zhai G, Valdes AM, Blackburn H, Mateo Leach I, de Boer RA, Kimura M, Aviv A, Wellcome Trust Case Control C, Goodall AH, Ouwehand W, van Veldhuisen DJ, van Gilst WH, Navis G, Burton PR, Tobin MD, Hall AS, Thompson JR, Spector T, Samani NJ (2010) Common variants near TERC are associated with mean telomere length. Nat Genet 42(3):197–199. doi:10.1038/ng.532
Cree LM, Patel SK, Pyle A, Lynn S, Turnbull DM, Chinnery PF, Walker M (2008) Age-related decline in mitochondrial DNA copy number in isolated human pancreatic islets. Diabetologia 51(8):1440–1443. doi:10.1007/s00125-008-1054-4
Short KR, Bigelow ML, Kahl J, Singh R, Coenen-Schimke J, Raghavakaimal S, Nair KS (2005) Decline in skeletal muscle mitochondrial function with aging in humans. Proc Natl Acad Sci USA 102(15):5618–5623. doi:10.1073/pnas.0501559102
Bonner MR, Shen M, Liu CS, DiVita M, He XZ, Lan Q (2009) Mitochondrial DNA content and lung cancer risk in Xuan Wei. China. Lung Cancer 63(3):331–334. doi:10.1016/j.lungcan.2008.06.012
Mengel-From J, Thinggaard M, Dalgard C, Kyvik KO, Christensen K, Christiansen L (2014) Mitochondrial DNA copy number in peripheral blood cells declines with age and is associated with general health among elderly. Hum Genet 133(9):1149–1159. doi:10.1007/s00439-014-1458-9
Miller FJ, Rosenfeldt FL, Zhang C, Linnane AW, Nagley P (2003) Precise determination of mitochondrial DNA copy number in human skeletal and cardiac muscle by a PCR-based assay: lack of change of copy number with age. Nucleic Acids Res 31(11):e61
Kim JH, Kim HK, Ko JH, Bang H, Lee DC (2013) The relationship between leukocyte mitochondrial DNA copy number and telomere length in community-dwelling elderly women. PLoS One 8(6):e67227. doi:10.1371/journal.pone.0067227
Tyrka AR, Carpenter LL, Kao HT, Porton B, Philip NS, Ridout SJ, Ridout KK, Price LH (2015) Association of telomere length and mitochondrial DNA copy number in a community sample of healthy adults. Exp Gerontol 66:17–20. doi:10.1016/j.exger.2015.04.002
Sahin E, Colla S, Liesa M, Moslehi J, Muller FL, Guo M, Cooper M, Kotton D, Fabian AJ, Walkey C, Maser RS, Tonon G, Foerster F, Xiong R, Wang YA, Shukla SA, Jaskelioff M, Martin ES, Heffernan TP, Protopopov A, Ivanova E, Mahoney JE, Kost-Alimova M, Perry SR, Bronson R, Liao R, Mulligan R, Shirihai OS, Chin L, DePinho RA (2011) Telomere dysfunction induces metabolic and mitochondrial compromise. Nature 470(7334):359–365. doi:10.1038/nature09787
Arigami T, Uenosono Y, Matsushita D, Yanagita S, Uchikado Y, Kita Y, Mori S, Kijima Y, Okumura H, Maemura K, Ishigami S, Natsugoe S (2016) Combined fibrinogen concentration and neutrophil-lymphocyte ratio as a prognostic marker of gastric cancer. Oncol Lett 11(2):1537–1544. doi:10.3892/ol.2015.4049
Acknowledgments
This work was supported by the National Natural Science Foundation of China (Grants 81320108021) and the National Basic Research Program of China (Grant 2015CB553703).
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Dengke Bao and Yanna Ba have contributed equally to this work.
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Supplementary Figure 1
Correlation between leukocyte mtDNA copy number and RTL (A), age, and relative telomere length (B) or mtDNA copy number (C). Values of mtDNA copy number were analyzed after log transformation. P values were calculated by Pearson’s correlation (TIFF 3023 kb)
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Bao, D., Ba, Y., Zhou, F. et al. Alterations of telomere length and mtDNA copy number are associated with overall survival in hepatocellular carcinoma patients treated with transarterial chemoembolization. Cancer Chemother Pharmacol 78, 791–799 (2016). https://doi.org/10.1007/s00280-016-3128-1
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DOI: https://doi.org/10.1007/s00280-016-3128-1