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RETRACTED ARTICLE: Atrial natriuretic peptide protects against cisplatin-induced granulocytopenia

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This article was retracted on 21 December 2023

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Abstract

Purpose

Granulocytopenia is the major toxicity associated with cisplatin treatment. Atrial natriuretic peptide (ANP) is a cardiac hormone used clinically for the treatment of acute heart failure in Japan. ANP exerts a wide range of protective effects on various organs, including the heart, blood vessels, lungs, and kidneys. This study’s objective was to investigate the protective effects of ANP on cisplatin-induced granulocytopenia in mice.

Methods

The mice were divided into two groups: cisplatin with vehicle and cisplatin with ANP. ANP (1.5 μg/kg/min via osmotic pump, subcutaneously) or vehicle administration was started 1 day before cisplatin injection until the mice were killed. At 0, 2, 4, 8, and 14 days after cisplatin injection (16 mg/kg, intraperitoneally as a single dose), the white blood cell, red blood cell, and platelet counts were measured in the peripheral blood in both groups. The numbers of total and live cells and colony-forming unit-granulocyte–macrophage (CFU-GM) colonies in the bone marrow of the mice were also examined. In addition, at 0, 0.5, 1, and 2 days after cisplatin injection, serum granulocyte colony-stimulating factor (G-CSF) levels were measured.

Results

ANP significantly attenuated the white blood cell count decrease in the peripheral blood 2 and 4 days after cisplatin injection. ANP also attenuated the decrease in the number of live cells and CFU-GM colonies in bone marrow 2, 4, and 8 days after cisplatin injection. ANP significantly increased serum G-CSF levels 1 day after cisplatin injection.

Conclusions

ANP has protective effects in cisplatin-induced granulocytopenia, with increased G-CSF production.

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Acknowledgments

The authors are grateful to M. Fukui for her technical assistance and to K. Shioya for helping us to care for the mice. This work was supported in part by research grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan (26861136); Osaka Cancer Society, Japan Research Foundation for Clinical Pharmacology, Kobayashi Foundation for Cancer Research, Mochida Memorial Foundation for Medical and Pharmaceutical Research, Uehara Memorial Foundation, the Senri Life Science Foundation, Kato Memorial Bioscience Foundation, and Takeda Science Foundation to T. Nojiri.

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Authors and Affiliations

  1. Department of Biochemistry, National Cerebral and Cardiovascular Center Research Institute, 5-7-1, Fujishirodai, Suita-City, Osaka, 565-8565, Japan

    Takashi Nojiri, Masahiro Zenitani, Takeshi Tokudome, Toru Kimura, Koichi Miura, Mikiya Miyazato & Kenji Kangawa

  2. Department of General Thoracic Surgery, Osaka University Graduate School of Medicine, Suita-City, Osaka, Japan

    Takashi Nojiri, Toru Kimura & Meinoshin Okumura

  3. Department of Regenerative Medicine and Tissue Engineering, National Cerebral and Cardiovascular Center, Suita-City, Osaka, Japan

    Hiroshi Hosoda

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  1. Takashi Nojiri
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  2. Hiroshi Hosoda
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  9. Kenji Kangawa
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Corresponding author

Correspondence to Takashi Nojiri.

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Nojiri, T., Hosoda, H., Zenitani, M. et al. RETRACTED ARTICLE: Atrial natriuretic peptide protects against cisplatin-induced granulocytopenia. Cancer Chemother Pharmacol 78, 191–197 (2016). https://doi.org/10.1007/s00280-016-3075-x

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  • DOI: https://doi.org/10.1007/s00280-016-3075-x

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