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Evaluation of the effects of different liquid inoculant formulations on the survival and plant-growth-promoting efficiency of Rhodopseudomonas palustris strain PS3

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Abstract

Biofertilizers can help improve soil quality, promote crop growth, and sustain soil health. The photosynthetic bacterium Rhodopseudomonas palustris strain PS3 (hereafter, PS3), which was isolated from Taiwanese paddy soil, can not only exert beneficial effects on plant growth but also enhance the efficiency of nutrient uptake from applied fertilizer. To produce this elite microbial isolate for practical use, product development and formulation are needed to permit the maintenance of the high quality of the inoculant during storage. The aim of this study was to select a suitable formulation that improves the survival and maintains the beneficial effects of the PS3 inoculant. Six additives (alginate, polyethylene glycol [PEG], polyvinylpyrrolidone-40 [PVP], glycerol, glucose, and horticultural oil) were used in liquid-based formulations, and their capacities for maintaining PS3 cell viability during storage in low, medium, and high temperature ranges were evaluated. Horticultural oil (0.5 %) was chosen as a potential additive because it could maintain a relatively high population and conferred greater microbial vitality under various storage conditions. Furthermore, the growth-promoting effects exerted on Chinese cabbage by the formulated inoculants were significantly greater than those of the unformulated treatments. The fresh and dry weights of the shoots were significantly increased, by 10–27 and 22–40 %, respectively. Horticultural oil is considered a safe, low-cost, and easy-to-process material, and this formulation would facilitate the practical use of strain PS3 in agriculture.

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Acknowledgments

We thank American Journal Experts for English language editing.

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Authors and Affiliations

  1. Department of Agronomy, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 106, Taiwan

    Sook-Kuan Lee & Huu-Sheng Lur

  2. Institute of Biotechnology, National Taiwan University, R412, No. 81, Chang-Xing St, Taipei, 106, Taiwan

    Sook-Kuan Lee, Huu-Sheng Lur, Kai-Jiun Lo, Kuan-Chen Cheng & Chi-Te Liu

  3. Graduate Institute of Food Science and Technology, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 106, Taiwan

    Kuan-Chen Cheng

  4. Agricultural Biotechnology Research Center, Academia Sinica, No. 128 Sec. 2, Academia Rd., Nankang, Taipei, 115, Taiwan

    Kuan-Chen Cheng & Chi-Te Liu

  5. Taoyuan District Agricultural Research and Extension Station, Council of Agriculture, Executive Yuan, 139, Sec. 2, Dongfu Rd., Houzhuang, Sinwu Dist., Taoyuan City, 32745, Taiwan

    Chun-Chao Chuang, Shiueh-Jung Tang & Zhi-Wei Yang

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  1. Sook-Kuan Lee
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  2. Huu-Sheng Lur
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  3. Kai-Jiun Lo
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  8. Chi-Te Liu
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Correspondence to Chi-Te Liu.

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Funding

This study was supported by grants from the Ministry of Science and Technology (MOST 103–2622-B-002-008-CC2 and MOST 102–2313-B-002-011-MY3) and National Taiwan University (103R7602B4) and was also funded by the Great Victory Chemical Industry Co. LTD.

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The authors declare that they have no conflicts of interest. This article does not contain any studies with human or animals participants.

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Sook-Kuan Lee and Huu-Sheng Lur contributed equally to the work.

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Lee, SK., Lur, HS., Lo, KJ. et al. Evaluation of the effects of different liquid inoculant formulations on the survival and plant-growth-promoting efficiency of Rhodopseudomonas palustris strain PS3. Appl Microbiol Biotechnol 100, 7977–7987 (2016). https://doi.org/10.1007/s00253-016-7582-9

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