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CYP287A1 is a carotenoid 2-β-hydroxylase required for deinoxanthin biosynthesis in Deinococcus radiodurans R1

  • Applied genetics and molecular biotechnology
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Abstract

The carotenoid deinoxanthin is a crucial resistance factor against various stresses in the radiation-resistant bacterium Deinococcus radiodurans. Disruption of the gene dr2473 encoding the cytochrome P450 CYP287A1 led to the accumulation of 2-deoxydeinoxanthin in D. radiodurans, demonstrating that CYP287A1 is a novel β-carotene 2-hydroxylase. The dr2473 knockout mutant was shown to be more sensitive to UV radiation and oxidative stress than the wild-type strain D. radiodurans R1, indicating that the C2 alcohol of deinoxanthin is important for antioxidant activity.

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Acknowledgments

This work was supported by grants from the National Basic Research (973) Program of China (2015CB755700), the National High-Tech (863) Program of China (No. 2012AA02A703), the National Research and Development Project of Transgenic Crops of China (2014ZX08009-003), the National Natural Science Foundation of China (No. 31170105), and the US National Science Foundation (MCB-0948751 to IM).

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Funding

This work was supported by grants from the National Basic Research (973) Program of China (2015CB755700), and the National High-Tech (863) Program of China (No. 2012AA02A703), and the National Research and Development Project of Transgenic Crops of China (2014ZX08009-003), the National Natural Science Foundation of China (No. 31170105), and the US National Science Foundation (MCB-0948751 to IM).

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The authors declare that they have no competing interests.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Authors and Affiliations

  1. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, People’s Republic of China

    Zhengfu Zhou, Wei Zhang, Shiyou Su, Ming Chen, Wei Lu, Min Lin & Yuquan Xu

  2. Natural Products Center, School of Natural Resources and the Environment, University of Arizona, Tucson, AZ, 85706, USA

    István Molnár

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  1. Zhengfu Zhou
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  2. Wei Zhang
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  3. Shiyou Su
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  4. Ming Chen
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  5. Wei Lu
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  6. Min Lin
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  7. István Molnár
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  8. Yuquan Xu
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Correspondence to István Molnár or Yuquan Xu.

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Zhengfu Zhou and Wei Zhang contributed equally to this work.

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Zhou, Z., Zhang, W., Su, S. et al. CYP287A1 is a carotenoid 2-β-hydroxylase required for deinoxanthin biosynthesis in Deinococcus radiodurans R1. Appl Microbiol Biotechnol 99, 10539–10546 (2015). https://doi.org/10.1007/s00253-015-6910-9

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  • DOI: https://doi.org/10.1007/s00253-015-6910-9

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