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Characterization and gene cloning of a maltotriose-forming exo-amylase from Kitasatospora sp. MK-1785

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An Erratum to this article was published on 25 February 2015

Abstract

A maltotriose-forming amylase (G3Amy) from Kitasatospora sp. MK-1785 was successfully isolated from a soil sample by inhibiting typical extracellular α-amylases using a proteinaceous α-amylase inhibitor. G3Amy was purified from the MK-1785 culture supernatant and characterized. G3Amy produced maltotriose as the principal product from starch and was categorized as an exo-α-amylase. G3Amy could also transfer maltotriose to phenolic and alcoholic compounds. Therefore, G3Amy can be useful for not only maltotriose manufacture but also maltooligosaccharide-glycoside synthesis. Further, the G3Amy gene was cloned and expressed in Escherichia coli cells. Analysis of its deduced amino acid sequence revealed that G3Amy consisted of an N-terminal GH13 catalytic domain and two C-terminal repeat starch-binding domains belonging to CBM20. It is suggested that natural G3Amy was subjected to proteolysis at N-terminal region of the anterior CBM20 in the C-terminal region. As with natural G3Amy, recombinant G3Amy could produce and transfer maltotriose from starch.

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Acknowledgments

This work was financially supported by grants-in-aid for scientific research (26450100) from the Japan Society for the Promotion of Science.

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Authors and Affiliations

  1. Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8531, Japan

    Masahiro Kamon, Jun-ichi Sumitani, Shuji Tani, Takashi Kawaguchi, M. Kamon, J. Sumitani, S. Tani & T. Kawaguchi

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  1. Masahiro Kamon
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  2. Jun-ichi Sumitani
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  4. Takashi Kawaguchi
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Correspondence to Jun-ichi Sumitani.

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Kamon, M., Sumitani, Ji., Tani, S. et al. Characterization and gene cloning of a maltotriose-forming exo-amylase from Kitasatospora sp. MK-1785. Appl Microbiol Biotechnol 99, 4743–4753 (2015). https://doi.org/10.1007/s00253-015-6396-5

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  • DOI: https://doi.org/10.1007/s00253-015-6396-5

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