Marine Biology

, Volume 158, Issue 5, pp 983–993

The sands of time: rediscovery of the genus Neozoanthus (Cnidaria: Hexacorallia) and evolutionary aspects of sand incrustation in brachycnemic zoanthids

  • James Davis Reimer
  • Mamiko Hirose
  • Yuka Irei
  • Masami Obuchi
  • Frederic Sinniger
Original Paper

DOI: 10.1007/s00227-011-1624-8

Cite this article as:
Reimer, J.D., Hirose, M., Irei, Y. et al. Mar Biol (2011) 158: 983. doi:10.1007/s00227-011-1624-8

Abstract

The zoanthid family Neozoanthidae (Anthozoa: Hexacorallia: Zoantharia) was described in 1973 from Madagascar as a monogeneric and monotypic taxon, and never reported again in literature. In 2008–2010, numerous zoanthid specimens fitting the morphological description of Neozoanthus were collected in the Ryukyu Islands, Okinawa, Japan, and the Great Barrier Reef (GBR), Australia. Utilizing these specimens, this study re-examines the phylogenetic position of Neozoanthidae and analyzes the evolutionary history of sand incrustation in zoanthids through phylogenetic and ancestral state reconstruction analyses. Specimens were colonial, partially incrusted with large, irregular sand and debris, zooxanthellate, and found from the intertidal zone to depths of approximately 30 m. Phylogenetic results utilizing mitochondrial 16S ribosomal DNA and cytochrome oxidase subunit I sequences show the presence of two Neozoanthus species groups, one each from Japan and the GBR. Unexpectedly, the molecular results also show Neozoanthus to be very closely related to the genus Isaurus, which as a member of the family Zoanthidae, is not sand incrusted. These results suggest that during evolution zoanthids can acquire and lose the ability to incrust sand with relative rapidity.

Supplementary material

227_2011_1624_MOESM1_ESM.tif (759 kb)
Fig. S1Scanning electron microscope image of a cross-section of specimen MISE 539 showing incrusted sponge spicules (ss) with relatively intact mesoglea (m) and remains of locations of other embedded materials (rough areas surrounding mesoglea). Scale = 200 μm. (TIFF 758 kb)
227_2011_1624_MOESM2_ESM.eps (366 kb)
Fig. S2Maximum likelihood (ML) tree of cytochrome oxidase subunit 1 (COI) sequences for zoanthid specimens. Values at branches represent ML probabilities (>50%) and Bayesian posterior probabilites (>0.50), respectively. Sequences newly obtained in this study in bold. Sequences/species names from previous studies in regular font with GenBank Accession Number. For specimen information see Table S1. (EPS 365 kb)
227_2011_1624_MOESM3_ESM.doc (58 kb)
Supplementary material 3 (DOC 58 kb)

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • James Davis Reimer
    • 1
    • 2
  • Mamiko Hirose
    • 1
  • Yuka Irei
    • 3
  • Masami Obuchi
    • 1
  • Frederic Sinniger
    • 1
    • 4
  1. 1.Molecular Invertebrate Systematics and Ecology Laboratory, Rising Star Program, Trans-Disciplinary Organization for Subtropical Island StudiesUniversity of the RyukyusOkinawaJapan
  2. 2.Marine Biodiversity Research Program, Institute of BiogeosciencesJapan Agency for Marine-Earth Science and Technology (JAMSTEC)KanagawaJapan
  3. 3.Molecular Invertebrate Systematics and Ecology Laboratory, Graduate School of Engineering and ScienceUniversity of the RyukyusOkinawaJapan
  4. 4.Molecular Ecology and Fisheries Genetics Laboratory, Environment Centre Wales Building, School of Biological SciencesBangor UniversityBangor, GwyneddUK