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Identification and quantification of six-ring C26H16 cata-condensed polycyclic aromatic hydrocarbons in a complex mixture of polycyclic aromatic hydrocarbons from coal tar

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Abstract

We applied a combination of normal-phase liquid chromatography (NPLC) with ultraviolet–visible spectroscopy and gas chromatography with mass spectrometry (GC/MS) for the fractionation, identification, and quantification of six ring C26H16 cata-condensed polycyclic aromatic hydrocarbons, PAHs, in the Standard Reference Material 1597a, Complex Mixture of PAHs from Coal Tar. For the characterization analysis, we calculated the GC retention indices of 17 C26H16 PAH authentic reference standards using the Rxi-PAH and DB-5 GC columns. Then, we used NPLC with ultraviolet–visible spectroscopy to isolate the fractions containing the C26H16 PAHs, and subsequently, we used GC/MS to establish the identity and quantity of the C26H16 PAHs using authentic reference standards. Following this procedure, 12 C26H16 cata-condensed PAHs benzo[c]pentaphene, dibenzo[f,k]tetraphene, benzo[h]pentaphene, dibenzo[a,l]tetracene, dibenzo[c,k]tetraphene, naphtho[2,3-c]tetraphene, dibenzo[a,c]tetracene, benzo[b]picene, dibenzo[a,j]tetracene, naphtho[2,1-a]tetracene, dibenzo[c,p]chrysene, and dibenzo[a,f]tetraphene were identified and quantified for the first time, and benzo[c]picene was quantified for the first time in an environmental combustion sample.

Identification and quantification of C26H16 cata-condensed PAHs in a complex mixture of coal tar

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Acknowledgments

The authors want to acknowledge Jacolin A. Murray from NIST for technical discussions on GC analyses of PAHs.

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    Authors and Affiliations

    1. National Institute of Standards and Technology (NIST), 100 Bureau Drive, Mail Stop 8390, Gaithersburg, MD, 20899, USA

      Jorge O. Oña-Ruales & Stephen A. Wise

    2. Department of Pharmacology, Penn State Hershey Cancer Institute, CH72, Penn State College of Medicine, 500 University Drive, Hershey, PA, 17033, USA

      Arun K. Sharma

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    1. Jorge O. Oña-Ruales
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    2. Arun K. Sharma
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    3. Stephen A. Wise
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    Correspondence to Jorge O. Oña-Ruales.

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    Certain commercial equipment, instruments, or materials (or suppliers, or software, …) are identified in this paper to foster understanding. Such identification does not imply recommendation or endorsement by the National Institute of Standards and Technology, NIST, nor does it imply that the materials or equipment identified are necessarily the best available for the purpose.

    Jorge O. Oña-Ruales, Arun K. Sharma and Stephen A. Wise contributed equally to this work.

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    Oña-Ruales, J.O., Sharma, A.K. & Wise, S.A. Identification and quantification of six-ring C26H16 cata-condensed polycyclic aromatic hydrocarbons in a complex mixture of polycyclic aromatic hydrocarbons from coal tar. Anal Bioanal Chem 407, 9165–9176 (2015). https://doi.org/10.1007/s00216-015-9084-5

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