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Biomarkers probed in saliva by surface plasmon resonance imaging coupled to matrix-assisted laser desorption/ionization mass spectrometry in array format

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Abstract

Detection of protein biomarkers is of major interest in proteomics. This work reports the analysis of protein biomarkers directly from a biological fluid, human saliva, by surface plasmon resonance imaging coupled to mass spectrometry (SPRi-MS), using a functionalized biochip in an array format enabling multiplex SPR-MS analysis. The SPR biochip presented a gold surface functionalized by a self-assembled monolayer of short poly(ethylene oxide) chains carrying an N-hydroxysuccinimide end-group for the immobilization of antibodies. The experiments were accomplished without any sample pre-purification or spiking with the targeted biomarkers. SPRi monitoring of the interactions, immune capture from the biochip surface, and finally on-chip matrix-assisted laser desorption/ionization-MS structural identification of two protein biomarkers, salivary α-amylase and lysozyme, were successively achieved directly from saliva at the femtomole level. For lysozyme, the on-chip MS identification was completed by a proteomic analysis based on an on-chip proteolysis procedure and a peptide mass fingerprint.

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Acknowledgements

CNRS, Evry-Val-d’Essonne University, and Genopole supported this work. Horiba Jobin Yvon is acknowledged for financial support.

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Authors and Affiliations

  1. CNRS, UMR8587, Laboratoire Analyse et Modélisation pour la Biologie et l’Environnement, 91025, Evry, France

    Johana Musso, William Buchmann, Florence Gonnet, Nathalie Jarroux & Regis Daniel

  2. Laboratoire Analyse et Modélisation pour la Biologie et l’Environnement, Université d’Evry-Val-d’Essonne, 91025, Evry, France

    Johana Musso, William Buchmann, Florence Gonnet, Nathalie Jarroux & Regis Daniel

  3. Horiba Scientific, 91120, Palaiseau, France

    Sophie Bellon, Chiraz Frydman & Didier-Luc Brunet

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  1. Johana Musso
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  2. William Buchmann
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  3. Florence Gonnet
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  4. Nathalie Jarroux
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  5. Sophie Bellon
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  6. Chiraz Frydman
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  7. Didier-Luc Brunet
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  8. Regis Daniel
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Correspondence to William Buchmann or Regis Daniel.

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Musso, J., Buchmann, W., Gonnet, F. et al. Biomarkers probed in saliva by surface plasmon resonance imaging coupled to matrix-assisted laser desorption/ionization mass spectrometry in array format. Anal Bioanal Chem 407, 1285–1294 (2015). https://doi.org/10.1007/s00216-014-8373-8

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  • DOI: https://doi.org/10.1007/s00216-014-8373-8

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