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Development of a monoclonal antibody-based, congener-specific and solvent-tolerable direct enzyme-linked immunosorbent assay for the detection of 2,2′,4,4′-tetrabromodiphenyl ether in environmental samples

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Abstract

A sensitive direct enzyme-linked immunosorbent assay (ELISA) for the specific detection of 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) in environmental samples was developed. A hapten mimicking BDE-47 was synthesized by introducing a butyric acid spacer into 5-hydroxy-BDE-47 and coupled to keyhole limpet hemocyanin to form an immunogen for the production of monoclonal antibodies (Mabs) against BDE-47. The most sensitive direct ELISA was formatted with a Mab, designated as 4F2, in combination with 5-(2,4-dibromophenoxy)pentanoic acid peroxidase as a tracer. The inhibition half-maximum concentrations and limit of detection of BDE-47 in phosphate buffered saline with 25% DMSO were 1.4 ± 0.05 and 0.1 ng mL−1, respectively. Cross-reactivity values of the ELISA with the tested BDE congeners and metabolites were ≤5.8%. This assay was used to determine BDE-47 in soil, sediment and house dust samples after ultrasonic extraction, simple cleanup and concentration steps. The average recoveries, repeatabilities (intraday extractions and analyses), and intra-laboratory reproducibilities (interday extractions and analyses) were in a range of 92–126%, 8–19% and 9–25%, respectively. Applied to 44 real samples, the results of this assay displayed a statistically significant correlation with those of a gas chromatography–mass spectrometry method (R 2 = 0.79-0.85), indicating this ELISA is a suitable tool for environmental analyses of BDE-47.

Direct competitive ELISA

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Acknowledgements

This work was supported in part by the National Natural and Science Foundation of China (No. 20977111) and the Chinese University Scientific Fund (No. 2009TD15).

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Authors and Affiliations

  1. College of Resources and Environmental Sciences, China Agricultural University, Beijing, 100193, China

    Jia Wang, Hao Li, Ji Li & Ting Xu

  2. USDA-ARS Biosciences Research Laboratory, 1605 Albrecht Boulevard, Fargo, ND, 58102, USA

    Weilin L. Shelver

  3. Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China

    Zhanhui Wang

  4. Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, 1955 East–west Road, Honolulu, HI, 96822, USA

    Qing X. Li

Authors
  1. Jia Wang
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  2. Hao Li
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  3. Weilin L. Shelver
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  4. Zhanhui Wang
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  5. Qing X. Li
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  6. Ji Li
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  7. Ting Xu
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Correspondence to Ting Xu.

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Wang, J., Li, H., Shelver, W.L. et al. Development of a monoclonal antibody-based, congener-specific and solvent-tolerable direct enzyme-linked immunosorbent assay for the detection of 2,2′,4,4′-tetrabromodiphenyl ether in environmental samples. Anal Bioanal Chem 401, 2249–2258 (2011). https://doi.org/10.1007/s00216-011-5283-x

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  • DOI: https://doi.org/10.1007/s00216-011-5283-x

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