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Homoeologous recombination-based transfer and molecular cytogenetic mapping of powdery mildew-resistant gene Pm57 from Aegilops searsii into wheat

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Pm57, a novel resistant gene against powdery mildew, was transferred into common wheat from Ae. searsi and further mapped to 2S s #1L at an interval of FL0.75 to FL0.87.

Abstract

Powdery mildew, caused by the fungus Blumeria graminis f. sp. tritici, is one of the most severe foliar diseases of wheat causing reduction in grain yield and quality. Host plant resistance is the most effective and environmentally safe approach to control this disease. Tests of a set of Chinese Spring–Ae. searsii (SsSs, 2n = 2x = 14) Feldman & Kislev ex K. Hammer disomic addition lines with a mixed isolate of the powdery mildew fungus identified a novel resistance gene(s), designed as Pm57, which was located on chromosome 2Ss#1. Here, we report the development of ten wheat–Ae. searsii recombinants. The wheat chromosomes involved in five of these recombinants were identified by FISH and SSR marker analysis and three of them were resistant to powdery mildew. Pm57 was further mapped to the long arm of chromosome 2Ss#1 at a fraction length interval of FL 0.75 to FL 0.87. The recombinant stocks T2BS.2BL-2Ss#1L 89-346 (TA5108) with distal 2Ss#1L segments of 28% and 89(5)69 (TA5109) with 33% may be useful in wheat improvement. The PCR marker X2L4g9p4/HaeIII was validated to specifically identify the Ae. searsii 2Ss#1L segment harboring Pm57 in T2BS.2BL-2Ss#1L against 16 wheat varieties and advanced breeding lines, and the development of more user-friendly KASP markers is underway.

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Acknowledgements

We thank Robert McIntosh and John Raupp for valuable suggestions and Duane L. Wilson for excellent technical assistance. This research was supported by the National Natural Science Foundation of China (31571658), the State Key Laboratory of Wheat and Maize Crop Science at Henan Agricultural University, Zhengzhou, China (39990022), the Kansas Wheat Commission, WGRC I/UCRC NSF contract 1338897 and the Kansas Crop Improvement Association. This paper is contribution number 17-077-J from the Kansas Agricultural Experiment Station, Kansas State University, Manhattan, KS 66506-5502.

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Authors and Affiliations

  1. College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, People’s Republic of China

    Wenxuan Liu, Qing Xia & Fuqiang Bai

  2. Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People’s Republic of China

    Chunxin Li & Yuli Song

  3. Department of Plant Pathology, Wheat Genetics Resource Center, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS, 66506-5502, USA

    Dal-Hoe Koo, Bernd Friebe & Bikram S. Gill

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  1. Wenxuan Liu
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  2. Dal-Hoe Koo
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  3. Qing Xia
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  4. Chunxin Li
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  7. Bernd Friebe
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  8. Bikram S. Gill
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Correspondence to Wenxuan Liu.

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Communicated by Aimin Zhang.

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Liu, W., Koo, DH., Xia, Q. et al. Homoeologous recombination-based transfer and molecular cytogenetic mapping of powdery mildew-resistant gene Pm57 from Aegilops searsii into wheat. Theor Appl Genet 130, 841–848 (2017). https://doi.org/10.1007/s00122-017-2855-y

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  • DOI: https://doi.org/10.1007/s00122-017-2855-y

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