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Allelism analysis of BrRfp locus in different restorer lines and map-based cloning of a fertility restorer gene, BrRfp1, for pol CMS in Chinese cabbage (Brassica rapa L.)

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Abstract

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In Chinese cabbage, there are two Rf loci for pol CMS and one of them was mapped to a 12.6-kb region containing a potential candidate gene encoding PPR protein.

Abstract

In Chinese cabbage (Brassica rapa), polima cytoplasmic male sterility (pol CMS) is an important CMS type and is widely used for hybrid breeding. By extensive test crossing in Chinese cabbage, four restorer lines (92s105, 01s325, 00s109, and 88s148) for pol CMS were screened. By analyzing the allelism of the four restorer lines, it was found that 92s105, 01s325, and 00s109 had the same “restorers of fertility” (Rf) locus (designated as BrRfp1), but 88s148 had a different Rf locus (designated as BrRfp2). For fine mapping the BrRfp1 locus of 92s105, a BC1F1 population with 487 individuals and a BC1F2 population with 2485 individuals were successively constructed. Using simple sequence repeat (SSR) markers developed from Brassica rapa reference genome and InDel markers derived from whole-genome resequencing data of 94c9 and 92s105, BrRfp1 was mapped to a 12.6-kb region containing a potential candidate gene encoding pentatricopeptide repeat-containing protein. Based on the nucleotide polymorphisms of the candidate gene sequence between the restoring and nonrestoring alleles, a co-segregating marker SC718 was developed, which would be helpful for hybrid breeding by marker-assisted screening and for detecting new restorer lines.

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Acknowledgements

This work was supported by grants from The National Key Research and Development Program of China (2016YFD0101701) and the National Science and Technology Support Program of China (2014BAD01B0802).

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Authors and Affiliations

  1. State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Huamin Zhang, Junqing Wu, Zihui Dai, Meiling Qin, Lingyu Hao, Yanjing Ren, Qingfei Li & Lugang Zhang

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  1. Huamin Zhang
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  2. Junqing Wu
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  3. Zihui Dai
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  4. Meiling Qin
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  7. Qingfei Li
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  8. Lugang Zhang
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Correspondence to Lugang Zhang.

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Communicated by C. F. Quiros.

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Zhang, H., Wu, J., Dai, Z. et al. Allelism analysis of BrRfp locus in different restorer lines and map-based cloning of a fertility restorer gene, BrRfp1, for pol CMS in Chinese cabbage (Brassica rapa L.). Theor Appl Genet 130, 539–547 (2017). https://doi.org/10.1007/s00122-016-2833-9

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  • DOI: https://doi.org/10.1007/s00122-016-2833-9

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