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Molecular characterization of waxy alleles in three subspecies of hexaploid wheat and identification of two novel Wx-B1 alleles

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Abstract

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Two novel Wx - B1 null alleles that enlarge the genetic variability for this wheat gene were characterized, whose effects on wheat quality could be different to those of the Wx - B1b allele.

Abstract

The starch composition of wheat grain has a primary influence on flour quality. Wheat starch consists of two types of glucose polymers: amylose (22–35 % of the total) and amylopectin (68–75 % of the total). Amylose is synthesized by waxy proteins. Several studies have contributed to the catalogue of waxy alleles available for breeders, and the search for novel alleles of these and other proteins related to flour quality continues. In this report, we describe the characterization of two novel Wx-B1 alleles (Wx-B1k and Wx-B1m) in a collection of macha, Indian dwarf and club wheat. Several accessions lacking Wx-B1 protein were detected, and some were caused by the common Wx-B1b null allele. Of the other accessions, four from Indian dwarf wheat showed the insertion of 4 bp within the seventh exon, and one from club wheat had a deletion of four nucleotides in the second exon. These mutations were novel and provisionally catalogued as Wx-B1k and Wx-B1m, respectively, and could be used to enlarge the genetic variability for this gene.

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References

  • Araki E, Miura H, Sawada S (2000) Differential effects of the null alleles at the three Wx loci on the starch-pasting properties of wheat. Theor Appl Genet 100:1113–1120

    Article  CAS  Google Scholar 

  • Badridze G, Lidner A, Asch F, Börner A (2009) Variation in salt tolerance within a Georgian wheat germplasm collection. Genet Resour Crop Evol 56:1125–1130

    Article  Google Scholar 

  • Chen SS, Chen GY, Chen H, Li YM, Liu YX, Liu DC, Lan XJ, Zheng YL (2012) Mapping stripe rust resistance gene YrSph derived from Triticum sphaerococcum Perc. with SSR, SRAP, and TRAP markers. Euphytica 185:19–26

    Article  CAS  Google Scholar 

  • Echt CS, Schwartz D (1981) Evidence for the inclusion of controlling elements within the structural gene at the waxy locus in maize. Genetics 99:275–284

    PubMed Central  CAS  PubMed  Google Scholar 

  • Filatenko AA, Hammer K (2014) Wheat landraces from Oman: a botanical analysis. Emir J Food Agric 26:119–136

    Google Scholar 

  • Giambanelli E, Ferioli F, Koçaoglu B, Jorjadze M, Alexieva I, Darbinyan N, D’Antuono LF (2013) A comparative study of bioactive compounds in primitive wheat populations from Italy, Turkey, Georgia, Bulgaria and Armenia. J Sci Food Agric 93:3490–3501

    Article  CAS  PubMed  Google Scholar 

  • Graybosch RA (1998) Waxy wheats: origin, properties, and prospects. Trends Food Sci Technol 9:135–142

    Article  CAS  Google Scholar 

  • Guzmán C, Alvarez JB (2012) Molecular characterization of a novel waxy allele (Wx-A u 1a) from Triticum urartu Thum. ex Gandil. Genet Resour Crop Evol 59:971–979

    Article  Google Scholar 

  • Guzmán C, Caballero L, Moral A, Alvarez JB (2010) Genetic variation for waxy proteins and amylose content in Spanish spelt wheat (Triticum spelta L.). Genet Resour Crop Evol 57:721–725

    Article  Google Scholar 

  • Guzmán C, Ortega R, Yamamori M, Peña RJ, Alvarez JB (2015) Molecular characterization of two novel null waxy alleles in Mexican bread wheat landraces. J Cereal Sci 62:8–14

    Article  Google Scholar 

  • Hayakawa K, Tanaka K, Nakamura T, Endo S, Hoshino T (2004) End use quality of waxy wheat flour in various grain-based foods. Cereal Chem 81:666–672

    Article  CAS  Google Scholar 

  • Hosono S (1954) The classification and the distribution of wheat. In: Kihara H (ed) Study of wheat. Yokendo, Tokyo, pp 5–132

    Google Scholar 

  • James MG, Denyer K, Myers AM (2003) Starch synthesis in the cereal endosperm. Curr Opin Plant Biol 6:215–222

    Article  CAS  PubMed  Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  CAS  PubMed  Google Scholar 

  • Li W, Liu A-J, Sheng Y-Z, Cheng G-Y, Pu Z-E, Liu Y-X, Kong L (2013) Genetic diversity of null alleles of waxy gene in Triticum L. J Plant Sci 8:15–23

    Article  Google Scholar 

  • Martin JM, Sherman JD, Lanning SP, Talbert LE, Giroux MJ (2008) Effect of variation in amylose content and puroindoline composition on bread quality in a hard spring wheat population. Cereal Chem 85:266–269

    Article  CAS  Google Scholar 

  • McIntosh RA, Yamazaki Y, Dubcovsky J, Rogers WJ, Morris C, Appels R, Xia XC (2013) Catalogue of gene symbols for wheat. http://www.shigen.nig.ac.jp/wheat/komugi/genes/macgene/2013/GeneSymbol.pdf. Accessed 13 Apr 2015

  • McLauchlan A, Ogbonnaya FC, Hollingsworth B, Carter M, Gale KR, Henry RJ, Holton TA, Morell MK, Rampling LR, Sharp PJ, Shariflou MR, Jones MGK, Appels R (2001) Development of robust PCR-based DNA markers for each homoeo-allele of granule-bound starch synthase and their application in wheat breeding programs. Aust J Agric Res 52:1409–1416

    Article  CAS  Google Scholar 

  • Miura H, Sugawara A (1996) Dosage effects of the three Wx genes on amylose synthesis in wheat endosperm. Theor Appl Genet 93:1066–1070

    Article  CAS  PubMed  Google Scholar 

  • Miura H, Tanii S (1994) Endosperm starch properties in several wheat cultivars preferred for Japanese noodles. Euphytica 72:171–175

    Article  Google Scholar 

  • Morell MK, Rahman S, Regina A, Appels R, Li Z (2001) Wheat starch biosynthesis. Euphytica 119:55–58

    Article  CAS  Google Scholar 

  • Nakamura T, Yamamori M, Hirano H, Hidaka S (1993) Identification of three Wx proteins in wheat (Triticum aestivum L.). Biochem Genet 31:75–86

    Article  CAS  PubMed  Google Scholar 

  • Nakamura T, Yamamori M, Hirano H, Hidaka S, Nagamine T (1995) Production of waxy (amylose-free) wheats. Mol Gen Genet 248:253–259

    Article  CAS  PubMed  Google Scholar 

  • Nakamura T, Vrinten P, Saito M, Konda M (2002) Rapid classification of partial waxy wheats using PCR-based markers. Genome 45:1150–1156

    Article  CAS  PubMed  Google Scholar 

  • Nakamura T, Shimbata T, Vrinten P, Saito M, Yonemaru J, Seto Y, Yasuda H, Takahama M (2006) Sweet wheat. Genes Genet Syst 81:361–365

    Article  CAS  PubMed  Google Scholar 

  • Ortega R, Guzmán C, Alvarez JB (2015) Molecular characterization of several Wx alleles in durum wheat. Biol Plant 59:220–226

    Article  CAS  Google Scholar 

  • Park CS, Baik BK (2007) Characteristics of French bread baked from wheat flours of reduced starch amylose content. Cereal Chem 84:437–442

    Article  CAS  Google Scholar 

  • Rahman S, Li Z, Batey I, Cochrane MP, Appels R, Morell M (2000) Genetic alteration of starch functionality in wheat. J Cereal Sci 31:91–110

    Article  CAS  Google Scholar 

  • Regina A, Bird A, Topping D, Bowden S, Freeman J, Barsby T, Kosar-Hashemi B, Li Z, Sadequr R, Morell M (2006) High-amylose wheat generated by RNA interference improves indices of large-bowel health in rats. Proc Natl Acad Sci USA 103:3546–3551

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Saito M, Nakamura T (2005) Two point mutations identified in emmer wheat generate null Wx-A1 alleles. Theor Appl Genet 110:276–282

    Article  CAS  PubMed  Google Scholar 

  • Saito M, Konda M, Vrinten P, Nakamura K, Nakamura T (2004) Molecular comparison of waxy null alleles in common wheat and identification of a unique null allele. Theor Appl Genet 108:1205–1211

    Article  CAS  PubMed  Google Scholar 

  • Saito M, Vrinten P, Ishikawa G, Graybosch R, Nakamura T (2009) A novel codominant marker for selection of the null Wx-B1 allele in wheat breeding programs. Mol Breed 23:209–217

    Article  CAS  Google Scholar 

  • Stacey J, Isaac P (1994) Isolation of DNA from plants. In: Isaac PG (ed) Methods in molecular biology: protocols for nucleic acid analysis by non-radioactive probes. Humana Press, Totawa, pp 9–15

    Google Scholar 

  • Vanzetti LS, Pfluger L, Bainotti CT, Jensen C, Helguera M (2010) Identification of a null allele at the Wx-A1 locus in durum wheat (Triticum turgidum L. ssp durum Desf.). Plant Breed 129:718–720

    Article  CAS  Google Scholar 

  • Vrinten P, Nakamura T, Yamamori M (1999) Molecular characterization of waxy mutations in wheat. Mol Gen Genet 261:463–471

    Article  CAS  PubMed  Google Scholar 

  • Yamamori M (2009) Amylose content and starch properties generated by five variant Wx alleles for granule-bound starch synthase in common wheat (Triticum aestivum L.). Euphytica 165:607–614

    Article  CAS  Google Scholar 

  • Yamamori M, Endo T (1996) Variation of starch granule proteins and chromosome mapping of their coding genes in common wheat. Theor Appl Genet 93:275–281

    Article  CAS  PubMed  Google Scholar 

  • Yamamori M, Quynh NT (2000) Differential effects of Wx-A1,-B1 and-D1 protein deficiencies on apparent amylose content and starch pasting properties in common wheat. Theor Appl Genet 100:32–38

    Article  CAS  Google Scholar 

  • Yamamori M, Yamamoto K (2011) Effects of two novel Wx-A1 alleles of common wheat (Triticum aestivum L.) on amylose and starch properties. J Cereal Sci 54:229–235

    Article  CAS  Google Scholar 

  • Yamamori M, Nakamura T, Endo TR, Nagamine T (1994) Waxy protein deficiency and chromosomal location of coding genes in common wheat. Theor Appl Genet 89–89:179–184

    Google Scholar 

  • Yamamori M, Nakamura T, Nagamine T (1995) Polymorphism of two waxy proteins in the emmer group of tetraploid wheat, Triticum dicoccoides, T. dicoccum, and T. durum. Plant Breed 114:215–218

    Article  CAS  Google Scholar 

  • Yamamori M, Kato M, Yui M, Kawasaki M (2006) Resistant starch and starch pasting properties of a starch synthase IIa-deficient wheat with apparent high amylose. Aust J Agric Res 57:531–535

    Article  CAS  Google Scholar 

  • Yasui T, Sasaki T, Matsuki J, Yamamori M (1997) Waxy endosperm mutants of bread wheat (Triticum aestivum L) and their starch properties. Breed Sci 47:161–163

    Google Scholar 

  • Zeng M, Morris CF, Batey IL, Wrigley CW (1997) Sources of variation for starch gelatinization, pasting, and gelation properties in wheat. Cereal Chem 74:63–71

    Article  CAS  Google Scholar 

  • Zhao XC, Batey IL, Sharp PJ, Crosbie G, Barclay I, Wilson R, Morell MK, Appels R (1998) A single genetic locus associated with starch granule properties and noodle quality in wheat. J Cereal Sci 27:7–13

    Article  CAS  Google Scholar 

  • Zwer PK, Sombrero A, Rickman RW, Klepper B (1995) Club and common wheat yield component and spike development in the Pacific Northwest. Crop Sci 35:1590–1597

    Article  Google Scholar 

Download references

Acknowledgments

This research was supported by grants AGL2010-19643-C02-01 and AGL2014-52445-R from the Spanish Ministry of Economy and Competitiveness, co-financed with European Regional Development Fund (FEDER) from the European Union. We thank the National Small Grain Collection (Aberdeen, USA) for supplying the analysed material. M. Ayala thanks for the pre-doctoral scholarship program ITAIPU Binacional-Paraguay (Paraguay Government).

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Authors and Affiliations

  1. Departamento de Genética, Escuela Técnica Superior de Ingeniería Agronómica y de Montes, Edificio Gregor Mendel, Campus de Rabanales, Universidad de Córdoba, 14071, Córdoba, Spain

    Marcela Ayala & Juan B. Alvarez

  2. División de Fitomejoramiento, Departamento de Producción Agrícola, Facultad de Ciencias Agrarias, Universidad Nacional de Asunción, San Lorenzo, Paraguay

    Marcela Ayala

  3. NARO Institute of Crop Science, National Agriculture and Food Research organization (NARO), Tsukuba, Ibaraki, Japan

    Makoto Yamamori

  4. Wheat Chemistry and Quality Laboratory, Global Wheat Program, International Maize and Wheat Improvement Center (CIMMYT), Texcoco, Mexico

    Carlos Guzmán

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  1. Marcela Ayala
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  2. Juan B. Alvarez
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  3. Makoto Yamamori
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  4. Carlos Guzmán
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Correspondence to Juan B. Alvarez.

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Communicated by E. Lagudah.

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Ayala, M., Alvarez, J.B., Yamamori, M. et al. Molecular characterization of waxy alleles in three subspecies of hexaploid wheat and identification of two novel Wx-B1 alleles. Theor Appl Genet 128, 2427–2435 (2015). https://doi.org/10.1007/s00122-015-2597-7

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  • DOI: https://doi.org/10.1007/s00122-015-2597-7

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