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Detection of novel QTLs for foxglove aphid resistance in soybean

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Abstract

Key message

The Raso2 , novel QTL for Korea biotype foxglove aphid resistance in soybean from PI 366121 was identified on chromosome 7 using GoldenGate SNP microarray.

Abstract

Foxglove aphid, Aulacorthum solani (Kaltenbach), is a hemipteran insect that infects a wide variety of plants worldwide and causes serious yield losses in crops. The objective of this study was to identify the putative QTL for foxglove aphid resistance in wild soybean, PI 366121, (Glycine soja Sieb. and Zucc.). One hundred and forty-one F4-derived F8 recombinant inbred lines developed from a cross of susceptible Williams 82 and PI 366121 were used. The phenotyping of antibiosis and antixenosis resistance was done through choice and no-choice tests with total plant damage and primary infestation leaf damage; a genome-wide molecular linkage map was constructed with 504 single-nucleotide polymorphism markers utilizing a GoldenGate assay. Using inclusive composite interval mapping analysis for foxglove aphid resistance, one major candidate QTL on chromosome 7 and three minor QTL regions on chromosomes 3, 6 and 18 were identified. The major QTL on chromosome 7 showed both antixenosis and antibiosis resistance responses. However, the minor QTLs showed only antixenosis resistance response. The major QTL mapped to a different chromosome than the previously identified foxglove aphid resistance QTL, Raso1, from the cultivar Adams. Also, the responses to the Korea biotype foxglove aphid were different for Raso1, and the gene from PI 366121 against the Korea biotype foxglove aphid was different. Thus, the foxglove aphid resistance gene from PI 366121 was determined to be an independent gene from Raso1 and was designated as Raso2. This result could be useful in breeding for new foxglove aphid-resistant soybean cultivars.

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Abbreviations

QTL:

Quantitative trait loci

SNP:

Single-nucleotide polymorphism

TPD:

Total plant damage

PLD:

Primary infestation leaf damage

RILs:

Recombinant inbred lines

DAI:

Days after infestation

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Acknowledgment

This work was carried out with the support of ‘Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ009771)’ Rural Development Administration, Republic of Korea.

Conflict of interest

The authors declare that they have no conflict of interest.

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    Authors and Affiliations

    1. Department of Crop Science and Biotechnology, Dankook University, Cheonan, 330-714, Korea

      Ju Seok Lee, Min-ho Yoo & Sungtaeg Kang

    2. National Institute of Crop Science, RDA, 151 Seodun-dong, Suwon, 441-857, Korea

      Jin Kyo Jung

    3. Plant Genetics Research Unit, USDA-ARS, 110 Waters Hall, Columbia, MO, 65211, USA

      Kristin D. Bilyeu

    4. Division of Plant Bioscience, Kyungpook National Univ, Daegu, 702-701, Republic of Korea

      Jeong-Dong Lee

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    1. Ju Seok Lee
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    2. Min-ho Yoo
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    Correspondence to Sungtaeg Kang.

    Additional information

    Communicated by D. A. Lightfoot.

    J. S. Lee and M. Yoo contributed equally to this work.

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    Lee, J.S., Yoo, Mh., Jung, J.K. et al. Detection of novel QTLs for foxglove aphid resistance in soybean. Theor Appl Genet 128, 1481–1488 (2015). https://doi.org/10.1007/s00122-015-2519-8

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