Abstract
Key message
A new gene for Ug99 resistance from wheat landrace PI 374670 was detected on the long arm of chromosome 7A.
Abstract
Wheat landrace PI 374670 has seedling and field resistance to stem rust caused by Puccinia graminis f. sp tritici Eriks. & E. Henn (Pgt) race TTKSK. To elucidate the inheritance of resistance, 216 BC1F2 families, 192 double haploid (DH) lines, and 185 recombinant inbred lines (RILs) were developed by crossing PI 374670 and the susceptible line LMPG-6. The parents and progeny were evaluated for seedling resistance to Pgt races TTKSK, MCCFC, and TPMKC. The DH lines were tested in field stem rust nurseries in Kenya and Ethiopia. The DH lines were genotyped with the 90K wheat iSelect SNP genotyping platform. Goodness-of-fit tests indicated that a single dominant gene in PI 374670 conditioned seedling resistance to the three Pgt races. The seedling resistance locus mapped to the long arm of chromosome 7A and this result was verified in the RIL population screened with the flanking SNP markers using KASP assays. In the same region, a major QTL for field resistance was detected in a 7.7 cM interval and explained 34–54 and 29–36 % of the variation in Kenya and Ethiopia, respectively. Results from tests with specific Pgt races and the csIH81 marker showed that the resistance was not due to Sr22. Thus, a new stem rust resistance gene or allele, either closely linked or allelic to Sr15, is responsible for the seedling and field resistance of PI 374670 to Ug99.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anderson JA, Ogihara Y, Sorrells ME, Tanksley SD (1992) Development of a chromosomal arm map for wheat based on RFLP markers. Theor Appl Genet 83:1035–1043
Cavanagh CR, Chao S, Wang S, Huang BE, Stephen S, Kiani S, Forrest K, Saintenac C, Brown-Guedira GL, Akunova A, See D, Bai G, Pumphrey M, Tomar L, Wong D, Kong S, Reynolds M, Lopez da Silva M, Bockelman H, Talbert L, Anderson JA, Dreisigacker S, Baenziger S, Carter A, Korzun V, Morrell PL, Dubcovsky J, Morrell MK, Sorrells ME, Hayden MJ, Akhunov E (2013) Genome-wide comparative diversity uncovers multiple targets of selection for improvement in hexaploid wheat landraces and cultivars. Proc Natl Acad Sci USA 110:8057–8062
Hale IL, Mamuya I, Singh D (2013) Sr31-virulent races (TTKSK, TTKST, and TTTSK) of the wheat stem rust pathogen Puccinia graminis f. sp. tritici are present in Tanzania. Plant Dis 97:557
Jin Y, Singh RP (2006) Resistance in U.S. wheat to recent eastern African isolates of Puccinia graminis f. sp. tritici with virulence to resistance gene Sr31. Plant Dis 90:476–480
Jin Y, Singh RP, Ward RW, Wanyera R, Kinyau M, Njau P, Fetch T, Pretorius ZA, Yahyaoui A (2007) Characterization of seedling infection types and adult plant infection responses of monogenic Sr gene lines to race TTKS of Puccinia graminis f. sp. tritici. Plant Dis 91:1096–1099
Jin Y, Szabo LJ, Pretorius ZA, Singh RP, Ward R, Fetch T (2008) Detection of virulence to resistance gene Sr24 within race TTKS of Puccinia graminis f. sp. tritici. Plant Dis 92:923–926
Jin Y, Szabo L, Rouse M, Fetch T, Pretorius ZA, Wanyera R, Njau P (2009) Detection of virulence to resistance gene Sr36 within race TTKS lineage of Puccinia graminis f.sp. tritici. Plant Dis 93:367–370
Knott DR (1989) The wheat rusts: breeding for resistance. Springer, Berlin
Knott DR (1990) Near-isogenic lines of wheat carrying genes for stem rust resistance. Crop Sci 30:901–905
Knott DR (2001) Anomalous segregation at the Sr6 locus for stem rust resistance in wheat. Theor Appl Genet 103:171–177
Kolodinska Brantestam A, Von Bothmer R, Dayteg C, Rashal I, Tuvesson S, Weibull J (2007) Genetic diversity changes and relationships in spring barley (Hordeum vulgare L.) germplasm of Nordic and Baltic areas as shown by SSR markers. Genet Resour Crop Evol 54:749–758
McIntosh RA, Wellings CR, Park RF (1995) Wheat rusts and the genetic bases of disease resistance. Wheat rusts: an atlas of resistance genes. CSIRO Publicatoins, East Melbourne, pp 1–28
Newcomb M, Acevedo M, Bockelman HE, Brown-Guedira G, Jackson EW, Jin Y, Njau P, Rouse MN, Singh D, Wanyera R, Goates BJ, Bonman JM (2013) Field resistance to the Ug99 race group of the stem rust pathogen in spring wheat landraces. Plant Dis 97:882–890
Olivera PD, Jin Y, Rouse M, Badebo A, Fetch T, Singh RP, Yahyaoui A (2012) Races of Puccinia graminis f. sp. tritici with combined virulence to Sr13 and Sr9e in a field stem rust screening nursery in Ethiopia. Plant Dis 96:623–628
Olson E, Brown-Guedira G, Marshall D, Stack E, Bowden RL, Jin Y, Rouse M, Pumphrey MO (2010) Development of wheat lines having a small introgressed segment carrying stem rust resistance gene Sr22. Crop Sci 50:1823–1830
Periyannan SK, Bansal UK, Bariana HS, Pumphrey M, Lagudah ES (2011) A robust molecular marker for the detection of shortened introgressed segment carrying the stem rust resistance gene Sr22 in common wheat. Theor Appl Genet 122:1–7
Peterson RF, Campbell AB, Hannah AE (1948) A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Can J Res 26:496–500
Pretorius ZA, Singh RP, Wagoire WW, Payne TS (2000) Detection of virulence to wheat stem rust resistance gene Sr31 in Puccinia graminis f.sp. tritici in Uganda. Plant Dis 84:203
Pretorius ZA, Bender CM, Visser B, Terefe T (2010) First report of a Puccinia graminis f. sp. tritici race virulent to the Sr24 and Sr31 wheat stem rust resistance genes in South Africa. Plant Dis 94:784
Pretorius ZA, Szabo LJ, Boshoff WHP, Herselman L, Visser B (2012) First report of a new TTKSF race of wheat stem rust (Puccinia graminis f. sp. tritici) in South Africa and Zimbabwe. Plant Dis 96:590
Roelfs AP, Singh RP, Saari EE (1992) Diseases of wheat: concepts and methods of disease management. CIMMYT, Mexico City
Rouse M, Jin Y (2011) Stem rust resistance in A-genome diploid relatives of wheat. Plant Dis 95:941–944
Rouse M, Wanyera R, Njau P, Jin Y (2011a) Sources of resistance to stem rust race Ug99 in spring wheat germplasm. Plant Dis 95:762–766
Rouse MN, Olson EL, Gill BS, Pumphrey MO, Jin Y (2011b) Stem rust resistance in Aegilops tauschii germplasm. Crop Sci 51:2074–2078
Rouse MN, Nirmala Y, Jin Y, Chao S, Fetch TG Jr, Pretorius ZA, Hiebert CW (2014) Characterization of Sr9h, a wheat stem rust resistance allele effective to Ug99. Theor Appl Genet 127:1681–1688
Schafer JF, Roelfs AP (1985) Estimated relation between numbers of urediniospores of Puccinia graminis f. sp. tritici and rates of occurrence of virulence. Phytopathology 75:749–750
Singleton LL, Moore MB, Wilcoxson RD, Lee M (1982) Evaluation of oat crown rust disease parameters and yield in moderately resistant cultivars. Phytopathology 72:538–540
Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114
Stakman EC, Steward DM, Loegering WQ (1962) Identification of physiologic races of Puccinia graminis var. tritici. USDA Agric Res Serv E-617, Washington, DC
Wang SD, Wong D, Forrest K, Allen A, Chao S, Huang E, Maccaferri M, Salvi S, Milner S, Cattievelli L, Mastrangelo AM, Whan A, Stephen S, Barker G, Wieseke R, Plieske J, Lillemo M, Mather D, Apples R, Dolferus R, Brown-Guedira G, Korol A, Akhunova AR, Feuillet C, Salse J, Morgante M, Pozniak C, Ming-Cheng L, Dvorak J, Morell M, Dubcovsky J, Ganal M, Tuberosa R, Lawley C, Mikoulitch I, Cavanagh C, Edwards KE, Hayden M, Akhunov E, International Wheat Genome Sequencing Consortium (2014) Characterization of polyploid wheat genomic diversity using a high-density 90 000 single nucleotide polymorphism array. Plant Biotechnol J 12:787–796
Wanyera R, Kinyua MG, Jin Y, Singh RP (2006) The spread of stem rust caused by Puccinia graminis f. sp. tritici, with virulence on Sr31 in wheat in Eastern Africa. Plant Dis 90:113
Watson IA, Luig NH (1966) Sr15—a new gene for use in the classification of Puccinia graminis var. tritici. Euphytica 15:239–250
Wolday A, Fetch T, Hodson DP, Cao W, Briere S (2011) First report of Puccinia graminis f. sp. tritici races with virulence on wheat stem rust resistance genes Sr31 and Sr24 in Eritrea. Plant Dis 95:1591
Acknowledgments
We are very grateful to Gebre Hiwot Abraha, Sam Stoxen, and Sam Gale for their technical assistance. This research was supported by the USDA-ARS National Plant Disease Recovery System, the USDA-ARS CRIS project 2050-21000-029-00D, and the Durable Rust Resistance in Wheat (DRRW) project managed by Cornell University and funded by the Bill and Melinda Gates Foundation and the United Kingdom Department for International development. This work was supported in part by National Research Initiative Competitive Grant 2011-68002-30029 (Triticeae-CAP) from the USDA National Institute of Food and Agriculture.
Conflict of interest
The authors declare no conflict of interest.
Author information
Authors and Affiliations
Corresponding authors
Additional information
Communicated by Patrick M. Hayes.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Babiker, E.M., Gordon, T.C., Chao, S. et al. Mapping resistance to the Ug99 race group of the stem rust pathogen in a spring wheat landrace. Theor Appl Genet 128, 605–612 (2015). https://doi.org/10.1007/s00122-015-2456-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00122-015-2456-6