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Mapping resistance to the bird cherry-oat aphid and the greenbug in wheat using sequence-based genotyping

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Identification of novel resistance QTL against wheat aphids. First QTL-resistance report for R. padi in wheat and chromosome 2DL for S. graminum . These sources have potential use in wheat breeding.

Abstract

The aphids Rhopalosiphum padi and Schizaphis graminum are important pests of common wheat (Triticum aestivum L.). Characterization of the genetic bases of resistance sources is crucial to facilitate the development of resistant wheat cultivars to these insects. We examined 140 recombinant inbred lines (RILs) from the cross of Seri M82 wheat (susceptible) with the synthetic hexaploid wheat CWI76364 (resistant). RILs were phenotyped for R. padi antibiosis and tolerance traits. Phenotyping of S. graminum resistance was based on leaf chlorosis in a greenhouse screening and the number of S. graminum/tiller in the field. RILs were also scored for pubescence. Using a sequence-based genotyping method, we located genomic regions associated with these resistance traits. A quantitative trait locus (QTL) for R. padi antibiosis (QRp.slu.4BL) that explained 10.2 % of phenotypic variation was found in chromosome 4BL and located 14.6 cM apart from the pubescence locus. We found no association between plant pubescence and the resistance traits. We found two QTLs for R. padi tolerance (QRp.slu.5AL and QRp.slu.5BL) in chromosomes 5AL and 5BL, with an epistatic interaction between a locus in chromosome 3AL (EnQRp.slu.5AL) and QRp.slu.5AL. These genomic regions explained about 35 % of the phenotypic variation. We re-mapped a previously reported gene for S. graminum resistance (putatively Gba) in 7DL and found a novel QTL associated with the number of aphids/tiller (QGb.slu-2DL) in chromosome 2DL. This is the first report on the genetic mapping of R. padi resistance in wheat and the first report where chromosome 2DL is shown to be associated with S. graminum resistance.

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Acknowledgments

We thank the Monsanto´s Beachell-Borlaug International Scholars Program for financial support. We also thank Estelle Proux-Wéra (SLU) for bioinformatic support through the PlantLink Network (SLU). We thank Jorge Montoya (CIMMYT) for assistance in conducting the field trial.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

The experiments comply with the current laws of the countries in which they were performed.

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Authors and Affiliations

  1. Department of Plant Breeding, Swedish University of Agricultural Sciences, P.O. Box 101, 23053, Alnarp, Sweden

    L. A. Crespo-Herrera, L. Garkava-Gustavsson & I. Åhman

  2. Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506-5502, USA

    E. Akhunov & K. W. Jordan

  3. Department of Entomology, Kansas State University, Manhattan, KS, 66506-5502, USA

    C. M. Smith

  4. International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6–641, 06600, Mexico DF, Mexico

    R. P. Singh

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  1. L. A. Crespo-Herrera
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  2. E. Akhunov
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  3. L. Garkava-Gustavsson
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  4. K. W. Jordan
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  5. C. M. Smith
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  6. R. P. Singh
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  7. I. Åhman
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Corresponding author

Correspondence to L. A. Crespo-Herrera.

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Communicated by Susanne Dreisigacker.

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Crespo-Herrera, L.A., Akhunov, E., Garkava-Gustavsson, L. et al. Mapping resistance to the bird cherry-oat aphid and the greenbug in wheat using sequence-based genotyping. Theor Appl Genet 127, 1963–1973 (2014). https://doi.org/10.1007/s00122-014-2352-5

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