Journal of Genetics

, Volume 85, Issue 2, pp 107–116

Highly conserved D-loop-like nuclear mitochondrial sequences (Numts) in tiger (Panthera tigris)

Authors

  • Wenping Zhang
    • Key Laboratory of Bioresource and Ecoenvironment (Ministry of Education), College of Life ScienceSichuan University
    • College of ScienceHonghe University
  • Zhihe Zhang
    • Key Laboratory for Reproduction and Conservation Genetics of Endangered Wildlife of Sichuan ProvinceChengdu Research Base of Giant Panda Breeding
  • Fujun Shen
    • Key Laboratory for Reproduction and Conservation Genetics of Endangered Wildlife of Sichuan ProvinceChengdu Research Base of Giant Panda Breeding
  • Rong Hou
    • Key Laboratory for Reproduction and Conservation Genetics of Endangered Wildlife of Sichuan ProvinceChengdu Research Base of Giant Panda Breeding
  • Xiaoping Lv
    • CITES Office
    • Key Laboratory of Bioresource and Ecoenvironment (Ministry of Education), College of Life ScienceSichuan University
Research Article

DOI: 10.1007/BF02729016

Cite this article as:
Zhang, W., Zhang, Z., Shen, F. et al. J Genet (2006) 85: 107. doi:10.1007/BF02729016

Abstract

Using oligonucleotide primers designed to match hypervariable segments I (HVS-1) ofPanthera tigris mitochondrial DNA (mtDNA), we amplified two different PCR products (500 bp and 287 bp) in the tiger (Panthera tigris), but got only one PCR product (287 bp) in the leopard (Panthera pardus). Sequence analyses indicated that the sequence of 287 bp was a D-loop-like nuclear mitochondrial sequence (Numts), indicating a nuclear transfer that occurred approximately 4.8–17 million years ago in the tiger and 4.6–16 million years ago in the leopard. Although the mtDNA D-loop sequence has a rapid rate of evolution, the 287-bp Numts are highly conserved; they are nearly identical in tiger subspecies and only 1.742% different between tiger and leopard. Thus, such sequences represent molecular ‘fossils’ that can shed light on evolution of the mitochondrial genome and may be the most appropriate outgroup for phylogenetic analysis. This is also proved by comparing the phylogenetic trees reconstructed using the D-loop sequence of snow leopard and the 287-bp Numts as outgroup.

Keywords

tigerD-loopNumtsevolution(NADH-5)translocation

Copyright information

© Indian Academy of Sciences 2006