Highly conserved D-loop-like nuclear mitochondrial sequences (Numts) in tiger (Panthera tigris)
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Using oligonucleotide primers designed to match hypervariable segments I (HVS-1) ofPanthera tigris mitochondrial DNA (mtDNA), we amplified two different PCR products (500 bp and 287 bp) in the tiger (Panthera tigris), but got only one PCR product (287 bp) in the leopard (Panthera pardus). Sequence analyses indicated that the sequence of 287 bp was a D-loop-like nuclear mitochondrial sequence (Numts), indicating a nuclear transfer that occurred approximately 4.8–17 million years ago in the tiger and 4.6–16 million years ago in the leopard. Although the mtDNA D-loop sequence has a rapid rate of evolution, the 287-bp Numts are highly conserved; they are nearly identical in tiger subspecies and only 1.742% different between tiger and leopard. Thus, such sequences represent molecular ‘fossils’ that can shed light on evolution of the mitochondrial genome and may be the most appropriate outgroup for phylogenetic analysis. This is also proved by comparing the phylogenetic trees reconstructed using the D-loop sequence of snow leopard and the 287-bp Numts as outgroup.
- Anderson S., Bankier A. T., Barrell B. G., de Bruijn M. H., Coulson A. R., Drouin J.et al. 1981 Sequence and organization of the human mitochondrial genome.Nature 290, 457–465. CrossRef
- Ankel-Simons F. and Cummins J. M. 1996 Misconception about mitochondria and mammalian fertilization: Implications for theories on human evolution.Proc. Natl. Acad. Sci. USA 93, 13859–13863. CrossRef
- Avise J. C. 1994Molecular markers, natural history and evolution. Chapman and Hall, New York.
- Bensasson D., Zhang D. X., Hartl D. L. and Hewitt G. M. 2001 Mitochondrial pseudogenes: evolution’s misplaced witnesses.Trends Ecol. Evol. 16, 314–321. CrossRef
- Blanchard J. L. and Schmidt G. W. 1995 Pervasive migration of organellar DNA to the nucleus in plants.J. Mol. Evol. 41, 397–406. CrossRef
- Campbell C. L. and Thorsness P. E. 1998 Escape of mitochondrial DNA to the nucleus inyme1 yeast is mediated by vacuolardependent turnover of abnormal mitochondrial compartments.J. Cell Sci. 11, 2455–2464.
- Cracraft J., Feinstein J., Vaughn J. and Helm-Bychowski K. 1998 Sorting out tigers (Panthera tigris): mitochondrial sequences, nuclear inserts, systematics, and conservation genetics.Anim. Conserv. 1, 139–150. CrossRef
- Cummins J. M., Wakayama T. and Yanagimachi R. 1997 Fate of microinjected spermatid mitochondria in the mouse oocyte and embryo.Zygote 5, 301–308. CrossRef
- DeWoody J. A., Chesser R. K. and Baker R. J. 1999 A translocated mitochondrial cytochromeb pseudogene in voles (Rodentia:Microtus).J. Mol. Evol. 48, 380–382. CrossRef
- Eyre-Walker A. and Awadalla P. 2001 Does human mtDNA recombine?J. Mol. Evol. 53, 430–435. CrossRef
- Gellissen G. and Michaelis G. 1987 Gene transfer: mitochondria to nucleus.Ann. N. Y. Acad. Sci. 503, 391–401. CrossRef
- Holland M. M. and Parsons T. J. 1999 Mitochondrial DNA sequence analysis-validation and use for forensic casework.Forensic Sci. Rev. 11, 22–50.
- Jae-Heup K., Eizirik E., O’Brien S. J. and Johnson W.E. 2001 Structure and patterns of sequence variation in the mitochondrial DNA control region of the great cats.Mitochondrion 1, 279–292. CrossRef
- Johnson W. E. and O’Brien S. J. 1997 Phylogenetic reconstruction of the Felidae using 16S rRNA andNADH-5 mitochondrial genes.J. Mol. Evol. 44 (suppl. 1), S98-S116. CrossRef
- Johnson W. E., Dratch P. A., Martenson J. S. and O’Brien S. J. 1996 Resolution of recent radiations within three evolutionary lineages of Felidae using mitochondrial restriction fragment length polymorphism variation.J. Mamm. Evol. 3, 97–120. CrossRef
- Kumar S., Tamura K., Jakobsen I. and Nei M. 2001 MEGA: molecular evolutionary genetics analysis. Ver2.0.Bioinformatics 17, 1244–1245. CrossRef
- Lopez J. V., Yuhki N., Masuda R., Modi W and O’Brien S. J. 1994Numt, a recent transfer and tandem amplification of mitochondrial DNA to the nuclear genome of the domestic cat.J. Mol. Evol. 39, 174–190.
- Lopez J. V., Cevario S. and O’Brien S. J. 1996 Complete nucleotide sequences of the domestic cat (Felis catus) mitochondrial genome and a transposed mtDNA tandem repeat (Numt) in the nuclear genome.Genomics 33, 229–246. CrossRef
- Lopez J. V., Culver M., Stephens J. C., Johnson W. E. and O’Brien S. J. 1997 Rates of nuclear and cytoplasmic mitochondrial DNA sequence divergence in mammals.Mol. Biol. Evol. 14, 277–286.
- Lu P., Zhang X., Lou S., Xu H. and Cheng Y 1998 Studying on allozyme of the Siberian tiger and the South China tiger. InA studying on South China tiger. pp. 156–159 (in Chinese).
- Naylor G. J., Collins P. and Brown W. M. 1995 Hydrophobicity and phylogeny.Nature 373, 565–566. CrossRef
- Seidensticker J. 1986 Large carnivores and the consequences of habitat insularization: Ecology and conservation of tigers in Indonesia and Bangladesh. In:Cats of the world: biology, conservation and management (ed. S. D. Miller and D. D. Everett), pp. 1–41. National Wildlife Federation, Washington.
- Sorenson M. D., and Fleischer R. C. 1996 Multiple independent transpositions of mitochondrial DNA control region sequences to the nucleus.Proc. Natl. Acad. Sci. USA 93, 15239–15243. CrossRef
- Sunnucks P. D. and Hales F. 1996 Numerous transposed sequences of mitochondrial cytochrome oxidase I-II in aphids of the genusSitobion (Hemiptera: Aphididae).Mol. Biol. Evol. 13, 510–524.
- Tan B. 1990A systematic list of the mammals, pp. 379–380. Medicine Science and Technology Press (in Chinese).
- Wallace D. C. 1995 Mitochondrial DNA variation in human evolution, degenerative disease and aging.Am. J. Hum. Genet. 57, 201–223.
- Wallace D. C., Stugard C., Murdock D., Schurr T. and Brown M. D. 1997 Ancient mtDNA sequences in the human nuclear genome: A potential source of errors in identifying pathogenic mutations.Proc. Natl. Acad. Sci. USA 94, 14900–14905. CrossRef
- Woischnik M. and Moraes C. T. 2002 Pattern of organization of human mitochondrial pseudogenes in the nuclear genome.Genome Res. 12, 885–893. CrossRef
- Wu P., Zhou K., Wang Y., Huan G. and Xu L. 1998 Studying the Siberian tiger and the South China tiger mtDNA polymorphism by RFLP and PCR-RFLP. InA studying on South China tiger, pp. 151–155 (in Chinese).
- Xu Y. 2001 Tiger (Panthera tigris) microsatellite polymorphisms and their application to the management of captive population. Doctoral paper of Northeast Forestry University, Harbin, China (in Chinese).
- Zhang D. X. and Hewitt G. M. 1996 Highly conserved nuclear copies of the mitochondrial control region in the desert locustSchistocerca gregaria: some implications for population studies.Mol Ecol. 5, 295–300. CrossRef
- Zhang Y.P. and Shi L. M. 1989 Mitochondrial DNA polymorphism in five species of the genusMacaca.Chinese J. Genet. 16, 325.
- Zhang X., Cheng Y., Zhu H. and Huang G. 1998 Comparing studying on chromosome of the Siberian tiger and the South China tiger. InA studying on South China tiger, pp. 160–164 (in Chinese).
- Zlschler H., Geisert H., von Haeseler A. and PÄÄbo S. 1995 A nuclear ‘fossil’of the mitochondrial D-loop and the origin of modern humans.Nature 378, 489–492. CrossRef
- Highly conserved D-loop-like nuclear mitochondrial sequences (Numts) in tiger (Panthera tigris)
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Volume 85, Issue 2 , pp 107-116
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- 1. Key Laboratory of Bioresource and Ecoenvironment (Ministry of Education), College of Life Science, Sichuan University, 610064, Chengdu, Sichuan, China
- 2. College of Science, Honghe University, 661100, Mengzi, Yunnan, China
- 3. Key Laboratory for Reproduction and Conservation Genetics of Endangered Wildlife of Sichuan Province, Chengdu Research Base of Giant Panda Breeding, 610084, Chengdu, Sichuan, China
- 4. CITES Office, China