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Probiotics promote rapid-turnover protein production by restoring gut flora in patients with alcoholic liver cirrhosis

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Abstract

Background and aims

Accumulating evidence suggests that deterioration of the gut flora contributes to the pathogenesis of alcoholic liver cirrhosis (ALC). However, the ALC flora profile and its response to probiotic treatment have not been fully examined. This double-blind placebo-controlled study aimed to evaluate whether the probiotic beverage Yakult 400 (Y400), which contains Lactobacillus casei strain Shirota, improves liver function in ALC patients, and to analyze the precise gut flora profile by real-time quantitative PCR (qPCR).

Methods

A total of 37 hospitalized ALC patients were randomly allocated to Y400 (n = 18) and placebo (n = 19) groups. Y400 or placebo was served twice a day during the first half of the four-week study. Serum concentrations of rapid-turnover proteins (i.e., transthyretin and transferrin), hypersensitive C-reactive protein, and interleukin-6 were measured weekly. qPCR analysis of fecal bacteria was performed biweekly; stocked fecal samples from 46 healthy subjects were used as references.

Results

Serum transthyretin levels significantly increased in the Y400 group in week 3; similar—although statistically insignificant—increases were seen for transferrin and albumin. Levels of hypersensitive C-reactive protein, but not interleukin-6, significantly decreased in week 4. Before treatment, populations of obligate anerobic bacteria were significantly smaller and those of Enterobacteriaceae were larger in patients than in healthy subjects examined in a previous study. Y400 corrected this imbalance.

Conclusions

This is the first report describing the unique gut flora of ALC patients. Y400 treatment normalized the gut flora and improved liver function. These promising findings warrant further investigation in larger, multicenter studies.

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Acknowledgments

The authors thank the nursing staff for collecting blood and fecal samples.

Conflict of interest

The authors have no potential conflicts of interest to disclose.

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Authors and Affiliations

  1. Division of Gastroenterology, Department of Medicine, Kurume University School of Medicine, 67 Asahi-machi, Kurume, 830-0011, Japan

    Hironori Koga, Yoshitaka Tamiya, Keiichi Mitsuyama & Michio Sata

  2. Orin Hospital, Onojo, Japan

    Masahiko Ishibashi

  3. Yakult Central Institute for Microbiological Research, Tokyo, Japan

    Satoshi Matsumoto, Akemi Imaoka, Taeko Hara, Masatoshi Nakano, Kazutoshi Ooeda & Yoshinori Umezaki

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  1. Hironori Koga
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  2. Yoshitaka Tamiya
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  4. Masahiko Ishibashi
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Correspondence to Hironori Koga.

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Koga, H., Tamiya, Y., Mitsuyama, K. et al. Probiotics promote rapid-turnover protein production by restoring gut flora in patients with alcoholic liver cirrhosis. Hepatol Int 7, 767–774 (2013). https://doi.org/10.1007/s12072-012-9408-x

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  • DOI: https://doi.org/10.1007/s12072-012-9408-x

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