Review

Cytotechnology

, Volume 51, Issue 2, pp 45-50

Identity tests: determination of cell line cross-contamination

  • C. M. CabreraAffiliated withStem Cell Bank of Andalucia (Spanish Central Node), Hospital Universitario Virgen de las Nieves Email author 
  • , F. CoboAffiliated withStem Cell Bank of Andalucia (Spanish Central Node), Hospital Universitario Virgen de las Nieves
  • , A. NietoAffiliated withStem Cell Bank of Andalucia (Spanish Central Node), Hospital Universitario Virgen de las Nieves
  • , J. L. CortésAffiliated withStem Cell Bank of Andalucia (Spanish Central Node), Hospital Universitario Virgen de las Nieves
  • , R. M. MontesAffiliated withStem Cell Bank of Andalucia (Spanish Central Node), Hospital Universitario Virgen de las Nieves
  • , P. CatalinaAffiliated withStem Cell Bank of Andalucia (Spanish Central Node), Hospital Universitario Virgen de las Nieves
  • , A. ConchaAffiliated withStem Cell Bank of Andalucia (Spanish Central Node), Hospital Universitario Virgen de las Nieves

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Abstract

Cell line cross-contamination is a phenomenon that arises as a result of the continuous cell line culture. It has been estimated that around 20% of the cell lines are misidentified, therefore it is necessary to carry out quality control tests for the detection of this issue. Since cell line cross-contamination discovery, different methods have been applied, such as isoenzyme analysis for inter-species cross-contamination; HLA typing, and DNA fingerprinting using short tandem repeat and a variable number of tandem repeat for intra-species cross-contamination. The cell banks in this sense represent the organizations responsible for guaranteeing the authenticity of cell lines for future research and clinical uses.

Keywords

Cell lines Cross-contamination Fingerprinting Isoenzyme analysis Short tandem repeat Variable number tandem repeat