Specific ultra-violet absorbance as an indicator of mercury sources in an Adirondack River basin
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- Burns, D.A., Aiken, G.R., Bradley, P.M. et al. Biogeochemistry (2013) 113: 451. doi:10.1007/s10533-012-9773-5
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The Adirondack region of New York has been identified as a hot spot where high methylmercury concentrations are found in surface waters and biota, yet mercury (Hg) concentrations vary widely in this region. We collected stream and groundwater samples for Hg and organic carbon analyses across the upper Hudson River, a 493 km2 basin in the central Adirondacks to evaluate and model the sources of variation in filtered total Hg (FTHg) concentrations. Variability in FTHg concentrations during the growing seasons (May–Oct) of 2007–2009 in Fishing Brook, a 66-km2 sub-basin, was better explained by specific ultra-violet absorbance at 254 nm (SUVA254), a measure of organic carbon aromaticity, than by dissolved organic carbon (DOC) concentrations, a commonly used Hg indicator. SUVA254 was a stronger predictor of FTHg concentrations during the growing season than during the dormant season. Multiple linear regression models that included SUVA254 values and DOC concentrations could explain 75 % of the variation in FTHg concentrations on an annual basis and 84 % during the growing season. A multiple linear regression landscape modeling approach applied to 27 synoptic sites across the upper Hudson basin found that higher SUVA254 values are associated with gentler slopes, and greater riparian area, and lower SUVA254 values are associated with an increasing influence of open water. We hypothesize that the strong Hg–SUVA254 relation in this basin reflects distinct patterns of FTHg and SUVA254 that are characteristic of source areas that control the mobilization of Hg to surface waters, and that the seasonal influence of these source areas varies in this heterogeneous basin landscape.
KeywordsMercury Dissolved organic carbon Aromatic SUVA Adirondack
Organic matter regulates mercury (Hg) cycling in the environment through numerous mechanisms including: ligand binding, effects on dissolution and precipitation, by serving as a microbial substrate, through its central role in redox processes, and as an indicator of hydrologic conditions (Gerbig et al. 2012). The strong association of Hg and organic matter in surface waters has long been recognized (Lindberg and Harriss 1974; Mierle and Ingram 1991), and numerous studies of Hg in streams and lakes of mid- and northern latitudes of the northern hemisphere have generally shown strong correlations between dissolved Hg concentrations and dissolved organic carbon (DOC) concentrations, and between particulate Hg concentrations and particulate organic carbon concentrations (POC) (Grigal 2002; Yin and Balogh 2002; Dennis et al. 2005; Brigham et al. 2009; Riscassi and Scanlon 2011). The association of Hg with organic matter is sufficiently strong in many surface waters, that measures such as DOC or absorbance at 254 nm (UV254) have been suggested as surrogates for Hg concentrations, because Hg analyses are expensive and may be unstable in automated water samplers (Dittman et al. 2009).
Interactions between Hg and organic matter are numerous and diverse, reflecting the complexity of natural organic matter (Ravichandran 2004; Gerbig et al. 2012). Strong binding of Hg by sulfur-bearing thiol groups has been shown, and is believed to be a strong driver of Hg association with dissolved organic matter (DOM) (Benoit et al. 2001; Skyllberg 2008). Hg may also be bound to organic matter as HgS nanoparticles that pass through 0.45 μm pore size filters, but this form may not be common in surface waters unimpacted by mining activities (Gerbig et al. 2011).
In natural waters, measures that reflect in part, the relative aromaticity of DOM such as the hydrophobic acid (HPOA) concentration or UV254, are often more strongly related to dissolved Hg than a general measure such as DOC or TOC (Shanley et al. 2008; Dittman et al. 2010; Riscassi and Scanlon 2011). Among several possible reasons for this association is that most reduced sulfur (and therefore, most of the strong Hg binding sites) is typically found within the HPOA fraction of DOM (Ravichandran 2004), which is more aromatic than the non-HPOA fraction. Additionally, the more aromatic fraction of organic matter may better stabilize Hg–S nanoparticles (Ravichandran et al. 1999; Gerbig et al. 2011). Thus, the association of Hg with the aromatic fraction of organic matter may be rooted in the complex chemistry of Hg interactions with natural organic matter. In addition to geochemistry-based reasons why Hg is more strongly associated with aromatic organic matter, this association may also originate through mobilization to surface waters and mixing of Hg and DOM from principal runoff source areas such as humic-rich shallow soils (Dittman et al. 2010) as well as riparian wetland soils (Ågren et al. 2008; Mierle and Ingram 1991), two locations where preferential storage of Hg has been identified (Grigal 2003). In summary, Hg–DOM chemistry, storage, and hydrology are interrelated in complex ways that are the subject of ongoing research; thus, determining the primacy of any single explanation for an association that is evident at the basin scale may be difficult.
Although dissolved Hg and DOC concentrations are often strongly correlated in many surface waters with r2 values of 0.7–0.9 (Grigal 2002; Shanley et al. 2008; Dittman et al. 2010; Riscassi and Scanlon 2011), not all waters show such strong a strong association. For example, Hg and DOC concentrations are weakly related in the Everglades where a diversity of organic matter sources and complex biogeochemical processes are observed (Aiken et al. 2011). Similarly, in surface waters strongly influenced by urban land use, diverse sources of Hg and DOC such as waste water treatment facilities often preclude a simple linear relation between dissolved Hg and DOC concentrations (Brigham et al. 2009).
The Adirondack region of New York is a largely forested and mountainous area with abundant lakes, streams, and wetlands. Surface waters in this region have been the frequent focus of investigations because of high sensitivity to acidification from acid deposition, and also due to high levels of Hg measured in surface waters and biota (Jenkins et al. 2005; Evers et al. 2007). Studies in Adirondack surface waters similar to those in nearby New England and southeastern Canada have shown a strong association of dissolved Hg and DOC concentrations (Driscoll et al. 1995; Selvendiran et al. 2009; Dittman et al. 2010). Many previously published investigations, however, have focused on small catchments or small lakes with drainage areas of only a few km2, and relatively homogeneous forested landscapes. Here, we describe results of a study in the 493 km2 upper Hudson River basin, which drains a largely forested area of diverse topography that includes mountainous terrain and riparian floodplains dominated by wetlands as well as numerous lakes and ponds interconnected by streams and rivers. Recently published studies in this basin have shown a relatively weak correlation between total Hg (THg) and DOC concentrations that varies seasonally and with flow (Bushey et al. 2008; Schelker et al. 2011; Burns et al. 2012), though some data in this basin indicate a strong Hg–DOC relation (Selvendiran et al. 2009; Dittman et al. 2010). We argue that the diverse landscape in this basin results in a range of organic matter sources and sinks that greatly affect the cycling of Hg and preclude the strong correlation between dissolved Hg and DOC concentrations often observed in smaller more homogeneous basins in this region. Here, we build on previous work that indicated specific ultra-violet absorbance at 254 nm (SUVA254) was a stronger predictor of variation in THg concentrations than were DOC concentrations during the summer across the upper Hudson basin (Burns et al. 2012). We hypothesize that SUVA254 is a potentially powerful predictor of dissolved Hg concentrations because this measure reflects the source areas and landscape locations where DOC and Hg interactions occur as well as the relative aromaticity and ability of the DOC to bind and transport Hg. Furthermore, we use a multiple regression spatial modeling approach to examine the relative influence of DOC concentrations and SUVA254 values on Hg concentrations, and use a similar approach to explore the landscape elements that most greatly affect variation in SUVA254 values. The results reported here may differ from those in small catchments because the Hg cycle in larger heterogeneous basins is affected by a diverse array of landscape types and varied sources and sinks of organic matter. Studies of Hg cycling in larger basins are important because these surface waters are more likely to have complex food webs that extend to larger carnivorous fish, which tend to have the highest Hg levels, and therefore pose the greatest human health risk.
Two principal data sets were analyzed in this paper: (1) Hg and organic carbon fraction concentrations in 43 stream samples collected from Fishing Brook at County Line Flow approximately every 3 weeks for 33-months from Jan 2007 to Sept 2009, and (2) SUVA254 values in 54 synoptic stream samples collected at 27 catchments scattered across the upper Hudson basin during spring (May) and summer (July and Aug) of 2006 and 2008. Data from the Fishing Brook gage were analyzed in sum and by season (dormant Nov 1–Apr 30, growing May 1–Oct 31) because seasonal patterns were observed in past analyses at this site (Schelker et al. 2011). Additionally, results from 22 samples collected at 7 groundwater seeps located in the largest riparian wetland within Fishing Brook (Fig. 1) are also discussed. Sampling, analytical, and data analysis methods for these samples are described below.
Fishing Brook was sampled at the outlet of County Line Flow, a small shallow impoundment just upstream of the USGS stream gage that recorded stage at 15-min intervals for conversion to streamflow through a rating curve (Rantz et al 1982). All stream samples were collected by wading to the center point of flow or reaching to the center of flow when channels were narrow or to sample groundwater seeps. Each bottle was rinsed three times with stream water before filling.
A trace metal sampling technique similar to EPA Method 1669 (US EPA 1996) was used for Hg that employed wrist-length nitrile (use of name brands in this paper is for identification purposes only and does not represent an endorsement by the U.S. government) gloves over shoulder-length polyethylene gloves. Samples were filtered through 0.7-μm pore size, prebaked (550 °C) quartz-fiber filters, acidified to approximately 1 % with 6 N HCl, and stored in the dark until analysis. The filters were placed in sterile petri dishes and stored frozen on dry ice until analysis. Additional details about Hg sampling methods are provided in Lewis and Brigham (2004) and QA/QC details are described in Schelker et al. (2011) and Burns et al. (2012).
Samples for DOC and UV254 were pressure-filtered through baked glass fiber 0.7-μm filters into 150 mL amber glass bottles and stored at 4 °C until analysis. The filter was also stored at 4 °C for later analysis of POC. Samples for HPOA analysis were passed through a 0.45-μm capsule filter into 1 L amber glass bottles and similarly stored at 4 °C until analysis.
Filtered total Hg (FTHg) and particulate total Hg (PTHg) were analyzed at the USGS Mercury Research Laboratory in Middleton, Wisconsin. Analysis of Hg was by cold vapor atomic fluorescence spectrometry according to the method described by Olson and DeWild (1999). THg is reported as the sum of FTHg and PTHg.
DOC concentrations, UV254 measurements, and DOC fractionation analyses were performed at the USGS Organic Carbon Laboratory in Boulder, Colorado. Dissolved organic carbon was analyzed on an OI Analytical Model 700 TOC analyzer (Aiken et al. 1992). UV254 measurements (absorbance at λ = 254 nm) were made with a Hewlett-Packard Photo-diode Array Spectrophotometer (model 8453) using a 10 mm quartz cell. UV254 data were corrected for the presence of iron (Fe) by subtracting the absorbance resulting from Fe(III) from the total absorbance value using an extinction coefficient of 0.0687; note that this coefficient is less than the value of 0.081–0.085 reported by Weishaar et al. (2003), and is based on more recent experimental data (B. Poulin personal communication). Analysis of Fe was done at the USGS National Water Quality Laboratory by Inductively-Coupled Plasma Emission Spectroscopy (USGS Method 01046, https:\\www.nemi.gov; accessed 17 Jan 2012). SUVA254 values were obtained by dividing the corrected UV254 value by the DOC concentration (mg C/L) and are reported in units of L/mg C/m. The HPOA fraction was obtained following established protocols (Aiken et al. 1992; Spencer et al. 2010). In brief, samples were acidified to pH 2 with HCl and passed through a column of XAD-8 resin. The HPOA fraction was retained on the XAD-8 resin and then back eluted with 0.1 M NaOH. The percent HPOA was determined by dividing the HPOA concentration by that of DOC. POC was analyzed at the USGS National Water Quality Laboratory by high temperature combustion according to EPA Method 440.0 (https:\\www.nemi.gov; accessed 17 Jan 2012).
The landscape metrics explored for inclusion in multiple regression models that best predict SUVA254 values across the 27 synoptic sites are the same as those discussed previously by Burns et al. (2012) for modeling THg and MeHg in the same basin. All of these metrics are described in Supplemental Table S-1, but only those that appeared in the best models are described here.
Landscape metrics were compiled for each sub-basin by geographic information system (GIS) analyses using Arc GIS software. The mean slope and mean topographic wetness index (TWI) were calculated for each sub-basin by terrain analysis of the 10 m National Elevation Dataset (http://seamless.usgs.gov, accessed 17 Jan 2012) using the System for Automated Geoscientific Analyses (SAGA, http://www.saga-gis.org/en/index.html, accessed 17 Jan 2012; Cimmery 2007). The TWI, ln (a/tan β), was first defined by Beven and Kirkby (1979), in which a is accumulated area per unit contour length and β is the local slope angle. Riparian area (expressed as proportion of total basin area and termed ripct) was calculated in SAGA by including all cells contributing to a given stream cell within an elevation <0.65 m above the stream cell. Wetland land cover was derived from the Adirondack Park Agency coverage, in which wetlands were delineated from 1:40,000 color infrared aerial photographs according to the approach used in the National Wetlands Inventory (Dahl et al. 2009; LaPoint et al. 2004).
Basin percent open water area is a commonly applied metric in studies of spatial controls on Hg cycling (Hurley et al. 1995; Shanley et al. 2005), and was explored here for inclusion in multiple regression models. An alternative to this metric, the open water index (OWI) that accounts for the proportion of basin drainage area that transits each open water body >0.025 km2 was also calculated (described in greater detail by Burns et al. 2012). The remaining small open water bodies (<0.025 km2 each) were compiled into a separate small open water body surface area metric for each sub-basin.
Multiple linear regression models were developed (SigmaStat software) to explore how well DOC concentrations and SUVA254 values together explained variation in FTHg concentrations at Fishing Brook at County Line Flow. A forward stepwise method was applied, and both independent variables were significant (p ≤ 0.05, with one noted exception) for all models discussed in the text. Additional multiple linear regression models were explored to determine which combination of the landscape metrics shown in Supplemental Table S-1 could best explain the variation in SUVA254 values across the 27 synoptic sites in the upper Hudson basin. Models were explored for every possible combination up to eight variables. A sequence of steps was then followed to select the “best” model. First, any models in which the p value of one of the independent variables was >0.05 were rejected. Second, any models that included a variance inflation factor (VIF) >4.0, a conservative level (O’Brien 2007), were rejected. The VIF is a measure of the proportion of variance accounted for by a given variable relative to the other independent variables in the model, and thus can help avoid including variables with high multi-collinearity, a common occurrence for landscape metrics. In general, any model with more than four predictive variables was rejected based on either p > 0.05 or VIF > 4.0. Any models remaining after these two steps were further evaluated by selecting those with the highest R2 values for each incremental number of independent variables, and then applying Akaike’s Information Criteria to select the best model with the least number of independent variables.
Results and discussion
Seasonal variation in Hg and organic carbon measures
Clear seasonal variation is apparent in the annual patterns of DOC and FTHg concentrations. For example, the highest annual DOC values of 9–12 mg/L were observed during summer (p < 0.001), when streamflow is typically lowest for the year. This type of seasonal pattern in DOC concentrations is commonly observed in watersheds dominated by near-stream wetlands (Schiff et al. 1998; Ågren et al. 2008), presumably due to: (1) waning influence of steeper upland areas with shallow soils during the driest seasonal conditions, and (2) increased influence from shallow groundwater in near-stream riparian areas with high DOC concentrations, where water tables remain relatively high in summer (Vidon and Hill 2004). In contrast, the greatest DOC and TOC concentrations in upland watersheds that lack significant near-stream wetlands or peat commonly occur during snowmelt and rain storms, driven by the flushing of organic carbon from shallow soils (Boyer et al. 1997; Hinton et al. 1997). Such watersheds often have low baseflow DOC concentrations of 1–5 mg/L with higher values during leaf fall (Mulholland 2003). Event-driven DOC flushing patterns are not readily apparent in Fishing Brook (Schelker et al. 2011). However, five samples collected at or near hydrograph peaks and marked with vertical dashed lines in Fig. 2a suggest that DOC concentrations sometimes increase during high flow relative to baseflow, though the broad seasonal pattern of higher concentrations during summer low flow appears stronger. This conclusion that seasonal dominance is a slightly stronger pattern than that of increases with flow during storms is confirmed by a significant (p = 0.036), but weak (r2 = 0.09) inverse least squares linear regression relation between log10 specific discharge and DOC concentrations for all samples collected at Fishing Brook; weak flow relations with DOC are a common finding in wetland-impacted streams (Hinton et al. 1997).
FTHg concentrations also were generally higher during the growing season than during the dormant season at 27 stream sites across the upper Hudson basin (Burns et al. 2012). Unlike DOC, however, FTHg concentrations increased with streamflow, a pattern observed during five different high flow periods marked with vertical dashed lines in Fig. 2a. Streamflow (log10 specific discharge) was significantly (p = 0.024) and positively related to FTHg concentrations, consistent with a source from flushing of shallow soils as hypothesized by Schelker et al. (2011), but this relation is relatively weak (r2 = 0.12), and explains little of the annual variation at Fishing Brook.
Some seasonality and response to flow conditions was observed in HPOA concentrations and SUVA254 values, but the patterns of the two differed (Fig. 2b). SUVA254 values sometimes followed the pattern of HPOA concentrations as in the latter half of 2008 and 2009, but sometimes show strikingly different patterns as in 2007. Overall, SUVA254 values and HPOA concentrations are significantly (p = 0.006) positively correlated, but the r2 value of 0.18 indicates a relatively weak relation. This relation improves only slightly to an r2 value of 0.22 (p = 0.002) when SUVA254 values are regressed with those of HPOA percent. Furthermore, SUVA254 values are even less strongly correlated with those of DOC concentrations (p = 0.10, r2 = 0.06) indicating independent variation among these two variables. In contrast, HPOA concentrations are highly correlated with those of DOC concentrations (p < 0.001, r2 = 0.92) indicating strong seasonal synchronicity among these two quantities.
Relation of FTHg concentrations to organic carbon measures
Linear regression relations for FTHg concentrations with organic carbon measures for all samples, dormant season, and growing season at Fishing Brook at County Line Flow during 2007–2009
Organic carbon measure
Dormant season (Nov 1–Apr 30)
Growing season (May 1–Oct 31)
Summary of studies that have reported FTHg–SUVA254 regression relations for surface waters
Fishing Brook, NY
One site; year round; n = 43
Burns et al. (2012)
Upper Hudson R, NY
Summer samples; 25 sites; sampled once ea
Tsui and Finlay (2011)
Summer samples; 30 sites; sampled once ea; regressions for log10 FTHg
Dittman et al. (2010)
Archer Ck, NY; Hubbard Bk, NH; Sleepers R, VT
Sites pooled for analysis; year round; total n = 128
Hall et al. (2008)
Lower Miss. R, LA
Lakes, rivers, and wetlands; 21 sites; sampled four times ea
Schuster et al. (2008)
Sleepers R, VT
Snowmelt samples; ten sites; total n = 57; SUVA254 relation for sub-set of data
Multiple regression models to predict FTHg concentrations
Seasonally, SUVA254 values and DOC concentrations are less effective at predicting FTHg concentrations during the dormant season (R2 = 0.55) than the growing season (R2 = 0.84) (Fig. 5b, c), though the dormant season model showed the most improvement relative to a bivariate regression with SUVA254 values. DOC concentrations explain more variation (ΔR2 = 0.42, p = 0.007) in FTHg concentrations during the dormant season than do SUVA254 values (ΔR2 = 0.12, p = 0.05), though both are significant dormant season predictors. This dormant season model shows greater bias than that of the annual model, underpredicting at high FTHg concentrations and overpredicting at low FTHg concentrations. In contrast, the stronger growing season model shows little bias, SUVA254 is the dominant predictive variable (ΔR2 = 0.82), and DOC concentrations add little predictive ability (ΔR2 = 0.02, p = 0.13).
The role of streamflow as an additional predictive variable in these models is quite different by season. The log10 specific discharge does not improve R2 values for the best growing season model, and has very low significance (p = 0.93), whereas log10 specific discharge is highly significant in the best dormant season model (p < 0.001). This latter model has an R2 value of 0.89, a strong improvement over the model that includes only the organic carbon measures (Fig. 5b). The low VIF value (1.1) for streamflow in the best dormant season model indicates that this variable accounts for variation in FTHg concentrations independently of the carbon measures. This finding is consistent with an hypothesis from earlier work at Fishing Brook that Hg is flushed from the shallow soil, primarily in riparian wetland areas during snowmelt and rain events, most evidently during the dormant season, and that this flushing mechanism appears to be independent of variations in DOC concentrations (Schelker et al. 2011), which are inversely and weakly related to flow as stated previously. In contrast, variation in growing season FTHg concentrations in Fishing Brook is strongly associated with variation in SUVA254 values, with little influence from either DOC concentrations or flow. This finding raises important questions as to the source areas and processes that affect variation in SUVA254 values in this basin, which are explored further in the next section.
Relation of SUVA254 values to landscape metrics
Given the apparent importance of SUVA254 values in explaining growing season variations in FTHg concentrations at Fishing Brook, the role of several landscape metrics are explored here to gain insight to the sources of variation in SUVA254 values across the upper Hudson basin. Multiple regression models that best account for variation in SUVA254 values were developed for synoptic samples collected at 27 stream sites across the upper Hudson River basin during 2006 and 2008 (Fig. 1). These sites range from steep upland watersheds to lowland streams with abundant riparian wetlands and varying influence from numerous open water bodies in the basin and are described in greater detail by Burns et al. (2012). Several of these sampling sites are within the Fishing Brook watershed discussed previously.
The multiple regression modeling results for synoptic samples collected in summer show some differences with those collected in spring (Fig. 6b). The best model in summer has an R2 of only 0.49, shows some bias (high at low SUVA254, low at high SUVA254), and indicates that about half the variation in SUVA254 values cannot be accounted for by these landscape metrics. This model includes slope and OWI as in the spring model, but also includes TWI and the small open water metric. Both accumulated area (analogous to drainage area) and slope are part of the TWI calculation, and reflect the tendency for a higher water table as values increase (Beven and Kirkby 1979). The TWI is inversely related (r2 = 0.19, p = 0.02) to slope in the upper Hudson basin, but is more strongly (and positively) related to the percent open water in each sub-basin (r2 = 0.27, p = 0.006), indicating that basins with high TWI values have gentler slopes, but also more open water. These two influencing factors are expected to have opposing effects on SUVA254 values.
Overall, three of four independent variables in the multiple regression model for SUVA254 based on summer synoptic samples are related in part to open water influence, and suggest stronger influence of open water on SUVA254 values in summer than in spring. The inverse relations with OWI and TWI indicate open water is associated with lower SUVA254 values. This finding suggests that low SUVA254 values may be derived in part from autochthonous processes such as decomposition of algae in the water column (Zumstein and Buffle 1989; Rostan and Cellot 1995). Photodegradation of allochthonous DOC may also contribute to declining SUVA254 values associated with open water influence (Minor and Stephens 2008). Regardless of the processes responsible, SUVA254 values often reach a minimum and the influence of autochthonous DOC reaches a maximum during summer low flow, particularly in lake-influenced systems (Zumstein and Buffle 1989; Hood et al. 2005) when sunlight and water temperature are at seasonal maximum values, consistent with the results presented here for summer synoptic samples. In contrast, the positive relation of SUVA254 values with the small open water variable is consistent with observations that these open waters are commonly beaver ponds or transitional wetlands in this basin, and thus behave more similarly to wetlands as a source of Hg (Driscoll et al. 1998) and higher SUVA254 values.
Although synoptic samples were generally collected during similar low-to-moderate flow conditions, flows were greater in spring (mean = 1.8 mm/day, RSD = 55 %) than in summer (mean = 1.3 mm/day, RSD = 109 %). Because DOC and Hg concentrations are often strongly related to streamflow and the latter may be similarly strongly related to SUVA254 values (Hood et al. 2006; Schuster et al. 2008; Dittman et al. 2010), specific discharge was considered as a potential additional explanatory variable in the best multiple regression SUVA254 models. Consideration of streamflow is further reinforced by a significant bivariate regression relation (r2 = 0.32, p = 0.002) between SUVA254 values and specific discharge for samples collected during the growing season at Fishing Brook. However, specific discharge was not significant (p > 0.05) in either spring or summer multiple regression synoptic models. Thus, inclusion of flow did not improve model predictions from those obtained with just landscape metrics. This analysis has limitations, however, because these synoptic samples do not well represent high flow conditions, nor is the role of storms reflected by the samples.
SUVA254 values are significantly inversely correlated with streamflow for samples collected in spring (r2 = 0.36, p = 0.002), and positively correlated for samples collected in summer (r2 = 0.26, p = 0.006). This difference may reflect the wetter conditions and higher water tables in this basin during spring than summer (Schelker et al. 2011). A previous study in Sweden in a similar heterogeneous landscape of upland forests and lowland riparian wetlands concluded that runoff in spring from forested upland areas (with lower SUVA254 values than in riparian areas) has a greater influence on stream DOC, and SUVA254 accordingly decreased with flow (Ågren et al. 2008). During summer, in contrast, when riparian saturation deficits are greatest, riparian influence increased with flow, and SUVA254 values accordingly increased with flow.
Open water influence on SUVA254 values can be similarly observed by examining the synoptic data by quartiles of the OWI (Fig. 7b). An analysis of variance indicated a statistically significant difference among these quartiles. A general decrease in SUVA254 values is observed across the quartile range, with the exception of the first to second quartiles. The highest quartile shows the lowest and statistically most distinct SUVA254 values (based on pairwise comparisons) similar to that observed for the highest percent riparian area quartile. Also, the range in median values among the OWI quartiles is slightly greater than that of the percent riparian area quartiles.
Relevance of results to methyl Hg transport
Although the analysis above focused on FTHg, filtered methyl Hg (FMHg) concentrations were also measured in the larger study from which this paper is derived, and these data have been discussed in previous papers (Bradley et al. 2011; Schelker et al. 2011; Burns et al. 2012). Broadly, FMHg concentrations are significantly related (p < 0.05) with FTHg concentrations and with SUVA254 values. These relations, however, do not have strong explanatory ability (r2 = 0.1–0.2), reflecting the influence of different processes that control the production of FMHg and FTHg as well as varying dependence on organic carbon fractions for transport of these Hg species to surface waters. Nonetheless, the discussion of FTHg transport is believed to have some broad transfer value to understanding FMHg transport as well because FTHg may be later transformed to FMHg.
The results of this study indicate an important role for SUVA254 as a predictor of temporal and spatial variation in FTHg concentrations in the upper Hudson River basin. At Fishing Brook, SUVA254 values explained twice the annual variation of FTHg concentrations than could be explained by DOC concentrations. Multiple regression models that included both variables accounted for an even greater amount of this variation in FTHg concentrations for all samples collected at Fishing Brook, but especially during the Nov–Apr dormant season. SUVA254 alone, however, was a notably stronger predictor of FTHg concentrations in the May–Oct growing season. This seasonal difference is believed to result largely from the opposing influence of (1) gentler slopes and more extensive riparian area as a source of higher SUVA254 values and FTHg concentrations, and (2) open water as a source (with the exception of small open water bodies) of lower SUVA254 values and FTHg concentrations. These data are also consistent with an additional role for increasing contributions of runoff originating from shallow flow paths during events associated with greater SUVA254 values and FTHg concentrations; however, the relationships of SUVA254, DOC, and FTHg with streamflow are relatively weak, suggesting that flow paths alone are only a minor explanatory variable (though samples collected in this study may not have adequately characterized stormflow). We believe these results generally reflect the manner in which the seasonal dynamics of upland versus riparian runoff sources combine with open water influence and flow-related variation to produce an apparent FTHg–SUVA relation. The aromaticity of DOC associated with higher SUVA254 values may contribute to the patterns observed here, but is probably not the most important factor because of the excess of strong Hg-binding sites generally available on DOC regardless of its relative aliphatic versus aromatic character. We believe these findings are especially pertinent to larger heterogeneous basins that encompass uplands, riparian wetlands, and open water bodies such as those commonly found in the northeastern US, southeastern Canada, and the upper Midwestern US (Lehner and Döll 2004), but these results await further confirmation through additional studies in large, heterogeneous basins.
Funding for this study was provided by the USGS National Water Quality Assessment Program. The authors thank Martyn Smith for GIS support. Thanks also to James Shanley and four anonymous reviewers for their constructive comments on an earlier version of this manuscript.
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