Original Article

Archives of Virology

, Volume 157, Issue 8, pp 1451-1461

Intranasal immunization with live attenuated influenza vaccine plus chitosan as an adjuvant protects mice against homologous and heterologous virus challenge

  • Xueliang WangAffiliated withShanghai Institute of Biological Products
  • , Wenjie ZhangAffiliated withXinhua Hospital affiliated to Shanghai Jiaotong University of Medicine Email author 
  • , Fen LiuAffiliated withShanghai Institute of Biological Products
  • , Mei ZhengAffiliated withShanghai Institute of Biological Products
  • , Dan ZhengAffiliated withShanghai Institute of Biological Products
  • , Tao ZhangAffiliated withState Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences
  • , Yinglei YiAffiliated withShanghai Institute of Biological Products
  • , Yahong DingAffiliated withShanghai Institute of Biological Products
  • , Jian LuoAffiliated withShanghai Institute of Biological Products
    • , Chaoyang DaiAffiliated withShanghai Institute of Biological Products
    • , Hanzhong WangAffiliated withState Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences
    • , Bing SunAffiliated withInstitute Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
    • , Ze ChenAffiliated withShanghai Institute of Biological ProductsState Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of SciencesCollege of Life Sciences, Hunan Normal University Email author 

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Abstract

Our previous studies have proven the adjuvanticity of chitosan in mice when administered with inactivated and subunit influenza vaccine. In this study, we investigated the adjuvant effect of chitosan on the immunogenicity and protective efficacy of a live attenuated influenza vaccine. Mice were inoculated intranasally with live attenuated influenza vaccine plus chitosan and then challenged with a high, lethal dose of homologous or heterologous virus. Antibody responses, secretion of IFN-γ by spleen cells, body weight loss, survival rates, and residual lung virus titers were tested. The results demonstrated that live attenuated influenza vaccine with chitosan adjuvant not only protected mice completely against challenge with the homologous virus but also provided good cross-protection against a heterologous virus. In addition, chitosan as adjuvant could significantly increase the levels of antigen-specific antibodies and the population of IFN-γ-secreting T cells. These results reveal the potential of chitosan as a candidate adjuvant for use in a live attenuated influenza vaccine.