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YAC Protocols

  • Book
  • © 2006

Overview

Editors:
  1. Alasdair MacKenzie

    1. School of Medical Sciences, College of Life Sciences and Medicine, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen, Scotland

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Part of the book series: Methods in Molecular Biology (MIMB, volume 349)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Markers, Selection, and Media in Yeast Artificial Chromosome Cloning

    • David Markie
    Pages 1-12

  3. Construction of Yeast Artificial Chromosome Libraries From Pathogens and Nonmodel Organisms

    • Cecilia P. Sanchez, Michael Lanzer
    Pages 13-26

  4. Analysis and Screening of Yeast Artificial Chromosome Libraries by Filter Hybridization

    • Cecilia P. Sanchez, Michael Lanzer
    Pages 27-32

  5. Analysis of Yeast Artificial Chromosome DNA by Restriction Digestion, Southern Blotting Nucleic Acid Hybridization, and Polymerase Chain Reaction

    • Sylvia Vasiliou, John Peter Quinn
    Pages 33-39

  6. Postgenomic Bioinformatic Analysis of Yeast Artificial Chromosome Sequence

    • Kerry Miller, Scott Davidson, Alasdair MacKenzie
    Pages 41-59

  7. Transformation of Yeast Using Bioactive Beads With Surface-Immobilized Yeast Artificial Chromosomes

    • Shigeki Kawakami, Satoshi Harashima, Akio Kobayashi, Kiichi Fukui
    Pages 61-65

  8. Modification and Amplification of Yeast Artificial Chromosomes

    • Sanbing Shen, Anthony Harmar, Nick Hastie
    Pages 67-74

  9. Modifying Yeast Artificial Chromosomes to Generate Cre/LoxP and FLP/FRT Site-Specific Deletions and Inversions

    • Gabriela G. Loots
    Pages 75-84

  10. Selective Isolation of Large Chromosomal Regions by Transformation-Associated Recombination Cloning for Structural and Functional Analysis of Mammalian Genomes

    • Natalay Kouprina, Vladimir N. Noskov, Vladimir Larionov
    Pages 85-101

  11. A Polymerase Chain Reaction-Mediated Yeast Artificial Chromosome-Splitting Technology for Generating Targeted Yeast Artificial Chromosomes Subclones

    • Yeon-Hee Kim, Minetaka Sugiyama, Yoshinobu Kaneko, Kiichi Fukui, Akio Kobayashi, Satoshi Harashima
    Pages 103-115

  12. Genomic Reconstruction by Serial Mitotic Recombination of Yeast Artificial Chromosomes

    • David Markie, Emma Jones, Jiannis Ragoussis
    Pages 117-126

  13. Targeting Vector Construction by Yeast Artificial Chromosome Modification

    • Peter J. Murray
    Pages 127-137

  14. Production of Yeast Artificial Chromosome Transgenic Mice by Pronuclear Injection of One-Cell Embryos

    • Alasdair MacKenzie
    Pages 139-150

  15. Generation of Yeast Artificial Chromosome Transgenic Mice by Intracytoplasmic Sperm Injection

    • Pedro N. Moreira, Julio Pozueta, Patricia Giraldo, Alfonso Gutiérrez-Adán, Lluís Montoliu
    Pages 151-161

  16. Establishment of Cell Lines That Exhibit Correct Ontogenic Stage-Specific Gene Expression Profiles From Tissues of Yeast Artificial Chromosome Transgenic Mice Using Chemically Induced Growth Signals

    • C. Anthony Blau, Kenneth R. Peterson
    Pages 163-173

  17. Application of Yeast Artificial Chromosomes in Fluorescence In Situ Hybridization

    • Thomas Liehr
    Pages 175-186

  18. Contribution of Yeast Artificial Chromosome-Based Physical Maps to the Final Assembly of the Trypanosoma cruzi Genome

    • Márcia R. M. Santos, Roberto R. M. Barros, Patrício E. Porcile, Mariano J. Levin, José Franco da Silveira
    Pages 187-197

  19. Back Matter

    Pages 199-203
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Keywords

About this book

It is now 10 years since the first edition of YAC Protocols was published in 1996. YAC Protocols was first produced to address the huge demand within the research community for a lab-based text that described in detail the wide range of uses for large insert yeast artificial chromosome (YAC) DNA clones. In doing this, the original editor, David Markie, and the many different contri- tors who provided descriptions of the protocols they used and developed, did a magnificent job. Indeed many of the techniques described within the first e- tion require little change and have stood up admirably to the test of time. Since the first edition, the use of YACs has proved invaluable for addressing a wide range of new biological problems ranging from those of basic biochemistry to assisting in the mapping and sequencing of the human genome. The requirement for a second edition of YAC Protocols was prompted by a number of major advances in biology since the publication of the first edition. These advances have included the sequencing of the human genome, and the genomes of a wide variety of other organisms, and the increased use of transgenic animals for understanding the molecular basis of human and animal disease.

Editors and Affiliations

  • School of Medical Sciences, College of Life Sciences and Medicine, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen, Scotland

    Alasdair MacKenzie

Bibliographic Information

  • Book Title: YAC Protocols

  • Editors: Alasdair MacKenzie

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1597451584

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2006

  • Hardcover ISBN: 978-1-58829-612-2Published: 18 August 2006

  • Softcover ISBN: 978-1-61737-673-3Published: 10 December 2010

  • eBook ISBN: 978-1-59745-158-1Published: 26 October 2007

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XVI, 204

  • Number of Illustrations: 49 b/w illustrations

  • Topics: Biochemistry, general, Human Genetics

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

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