Abstract
After ischemic stroke, microRNAs (miRNAs) participate in various processes, including immune responses, inflammation, and angiogenesis. Diabetes is a key factor increasing the risk of ischemic stroke; however, the regulatory pattern of miRNAs at different stages of diabetic stroke remains unclear. This study comprehensively analyzed the miRNA expression profiles in diabetic mice at 1, 3, and 7 days post-reperfusion following the middle cerebral artery occlusion (MCAO). We identified differentially expressed (DE) miRNAs in diabetic stroke and found significant dysregulation of some novel miRNAs (novel_mir310, novel_mir89, and novel_mir396) post-stroke. These DEmiRNAs were involved in apoptosis and the formation of tight junctions. Finally, we identified three groups of time-dependent DE miRNAs (miR-6240, miR-135b-3p, and miR-672-5p). These have the potential to serve as biomarkers of diabetic stroke. These findings provide a new perspective for future research, emphasizing the dynamic changes in miRNA expression after diabetic stroke and offering potential candidates as biomarkers for future clinical applications.
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Data Availability
The datasets generated for this study can be found in the Genome Sequence Archive with accession numbers CRA013152 and CRA014257.
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Funding
This work was supported by the Nation Natural Science Foundation of China (grant no. 82071405), the Natural Science Foundation of Hubei province (no.2020CFB707) and the Foundation of Health Commission of Hubei province (no.WJ20210038).
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All authors contributed to the study conception and design. Material preparation, data collection were performed by Guanghui Xie. Yifei Lv finished analysis and wrote the main manuscript text. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Experimental Animal Ethics Committee of the Zhongnan Hospital of Wuhan University. For detailed information on relevant ethical standards and criteria, please refer to the sections on research involving animals, their data or biological material.
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Lv, Y., Xie, G., Xi, Y. et al. MicroRNA Regulatory Pattern in Diabetic Mouse Cortex at Different Stages Following Ischemic Stroke. J Mol Neurosci 74, 36 (2024). https://doi.org/10.1007/s12031-024-02207-5
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DOI: https://doi.org/10.1007/s12031-024-02207-5