Abstract
Embryogenesis is the critical developmental phase in the life cycle of flowering plants, which starts with the double fertilization. In the model plant Arabidopsis, since the single-celled zygote follows a defined cell division and differentiation pattern to form a mature embryo, Arabidopsis embryogenesis is an ideal system for research of the cell fate determination and cell–cell communication. Here, we present an efficient semi–in vivo zygotic embryogenesis system in Arabidopsis via ovule culture. To achieve highly efficient embryogenesis, some key factors were carefully investigated. B5 medium supplemented with 10% sucrose was suitable for embryogenesis. After 5 d of culture, about 43% of all ovules could develop from the zygote stage to the globular embryo stage. By applying the system, we found that exogenous spermidine was not required for embryo development in ovules, and dicyclohexylamine, an inhibitor of spermidine synthase, significantly inhibited embryogenesis, and the inhibiting effect could be rescued by exogenous spermidine. Also, we tested the development of the ovules at 1-celled embryo stage after laser microdissection of apical cell, confirming it a useful system for experimental embryological investigations.
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This work is supported by the National Nature Science Foundation (30800086).
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Li, J., Li, S., Liu, Y. et al. An efficient semi–in vivo zygotic embryogenesis system in Arabidopsis. In Vitro Cell.Dev.Biol.-Plant 59, 209–215 (2023). https://doi.org/10.1007/s11627-022-10319-z
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DOI: https://doi.org/10.1007/s11627-022-10319-z