Abstract
This article reports an efficient protocol for the indirect organogenesis of Sechium edule, a Mesoamerican species with important nutritional and pharmaceutical characteristics. The in vitro establishment was carried out with hypocotyl segments; subsequently, the formation of calluses was induced from leaves and nodal stem segments using different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D; 0.5, 1.0, and 1.5 mg L−1). Then, shoot formation was induced using different concentrations of thidiazuron (TDZ; 0.5, 1.0, and 1.5 mg L−1) in medium supplemented with 1.0 mg L−1 benzylaminopurine (BAP). Different concentrations of 1-naphthaleneacetic acid (NAA; 1.0, 2.0, and 3.0 mg L−1) were assessed to stimulate shoot rooting; chlorophyll-a, chlorophyll-b, and total chlorophyll were quantified during this stage. The results showed that callus formation and weight increased in parallel with 2,4-D concentration, with nodal segments responding favorably. Shoot regeneration was higher in medium supplemented with 0.5 mg L−1 TDZ plus 1.0 mg L−1 BAP. Shoot elongation and rooting were greater using 1.0 mg L−1 NAA, while chlorophyll-a, chlorophyll-b, and total chlorophyll contents were higher in medium without NAA. A 90% plant survival was recorded after acclimatization.
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Acknowledgements
Thanks to the Consejo Nacional de Ciencia y Tecnología (National Council of Science and Technology) for the grant to A.S.C. 932915. Also thanks to Dr. Eucario Mancilla Alvarez for the support to the investigation.
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Soto-Contreras, A., Núñez-Pastrana, R., Rodríguez-Deméneghi, M.V. et al. Indirect organogenesis of Sechium edule (Jacq.) Swartz. In Vitro Cell.Dev.Biol.-Plant 58, 903–910 (2022). https://doi.org/10.1007/s11627-022-10304-6
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DOI: https://doi.org/10.1007/s11627-022-10304-6