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Towards ex situ conservation of globally rare Turkish endemic Tripleurospermum fissurale (Asteraceae)

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Abstract

A rapid and efficient in vitro micropropagation system was developed to conserve Tripleurospermum fissurale (Sosn.) E.Hossain (Asteraceae), a rare endemic species for Turkey. Murashige and Skoog basal medium (MS) supplemented with 4.7 µM KIN was found to be the most appropriate basal medium with a 60% germination rate. MS medium supplemented with 4.9 µM 2iP and 0.5 µM IBA gave the highest shoot length of 54.3 ± 3.53 mm. Furthermore, 4.4 µM 6-BA combined with 0.5 µM IBA was superior for the highest shoot number with 3.4 ± 0.49 after 4-wk culture. Cytogenetic analyses indicated that all propagated plants have the same DNA ploidy level (x) and chromosome number (2n = 18) compared with the mother plants. After 4 wk, the rooting percentage achieved 100% in all tested rooting media. MS medium supplemented with 2.7 µM NAA favored the highest root number, root length, and secondary root number with 3.44 ± 0.5, 170.1 ± 4.91 mm, and 26.1 ± 1.65, respectively. Rooted plantlets were initially acclimatized and then transferred in greenhouse conditions. This method could be evaluated for ex situ conservation of rare endemic and endangered plant species.

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Acknowledgements

The authors thank the greenhouse personnel’s of Karadeniz Technical University, Faculty of Forest for ex vitro studies. We are grateful to Prof. Dr. Atalay Sökmen for reading the manuscript and for his valuable comments.

Funding

This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK project no (grant numbers) 117Z588 and 106T162).

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Authors and Affiliations

  1. Department of Food Technology, Şebinkarahisar School of Applied Sciences, Giresun University, 28400, Giresun, Turkey

    Mustafa Cuce

  2. Department of Biology, Faculty of Science, Karadeniz Technical University, 61080, Trabzon, Turkey

    Huseyin Inceer, Kemal Vehbi Imamoglu & Tugba Ergin

  3. Department of Forest Engineering, Faculty of Forestry, Karadeniz Technical University, 61080, Trabzon, Turkey

    Ali Omer Ucler

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  1. Mustafa Cuce
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  2. Huseyin Inceer
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Correspondence to Mustafa Cuce.

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Cuce, M., Inceer, H., Imamoglu, K.V. et al. Towards ex situ conservation of globally rare Turkish endemic Tripleurospermum fissurale (Asteraceae). In Vitro Cell.Dev.Biol.-Plant 58, 1002–1011 (2022). https://doi.org/10.1007/s11627-022-10289-2

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