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Elicitor-induced production of aervine in adventitious shoot cultures of Aerva lanata (L.) Juss. Ex Schult. and its biological applications

  • Plant Tissue Culture
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Abstract

Aerva lanata L. with enhanced aervine content can be used as an alternative source of antioxidative and antibacterial compounds through in vitro cultures. This study discusses a new method of aervine production using elicitors in A. lanata adventitious shoot culture. The elicitation of aervine in suspension culture was tested using five elicitors at different concentrations, and the most effective enhancement was recorded on the 20th d of treatment. Multiple shoot development was recorded for nodal explants (5.33 ± 1.15) in the Murashige and Skoog (MS) medium containing 1.0 mg L−1 benzylaminopurine (BAP) and 0.5 mg L−1 kinetin (KIN). Among the elicitors, chitosan (50 mg L−1) and NaH2PO4 (25 mg L−1) produced a 1.26- and 0.79-fold increase in the aervine content than the control. The elicited extract was subjected to liquid chromatography-mass spectrometry analysis to confirm the presence of aervine and the amount was quantified using high-performance liquid chromatography (HPLC). Aervine showed superior scavenging activity in DPPH (2,2-diphenyl-1-picrylhydrazyl) (IC50 of 42.40 ± 0.79 μg mL−1) and ABTS (2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) (43.73 ± 0.17) assays. In addition, it showed a prominent antibacterial activity against pathogenic gram-positive and gram-negative bacteria, such as Escherichia coli, Bacillus subtilis, and Proteus vulgaris. For the first time, this study revealed the antibacterial properties of enhanced aervine content from shooting culture.

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References

  • Agarwal M (2015) Tissue culture of Momordica charantia L.: a review. J Plant Sci 3:24–32

    Google Scholar 

  • Ahmad S, Garg M, Tamboli ET, Abdin MZ, Ansari SH (2013) In vitro production of alkaloids: factors, approaches, challenges, and prospects. Pharmacogn Rev 7:27–33

    Article  Google Scholar 

  • Al-Ansari M, Al-Humaid LA, Vijayaraghavan P, Ravindran B, Chang SW, Agastian P, Rathi MA, Balamuralikrishnan B (2019) Identification of phytochemical components from Aerva lanata (Linn.) medicinal plants and it’s in vitro inhibitory activity against drug-resistant microbial pathogens and antioxidant properties. Saudi J Biol Sci 26:129–1133

    Article  Google Scholar 

  • Aldulaimi KO, Azziz SS, Bakri YM, Nafiah MA, Aowda SA, Awang K, Litaudon M (2019) Two new isoquinoline alkaloids from the bark of Alphonsea cylindrica King and their antioxidant activity. Phytochem Lett 29:110–114

    Article  Google Scholar 

  • Anand S, Jayakumar E, Jeyachandran R, Nandagobalan V, Doss A (2012) Direct organogenesis of Passiflora foetida L. through nodal explants. Plant Tiss Cult Biotechol 22:87–91

    Article  Google Scholar 

  • Arunachalam K, Parimelazhagan T (2013) Anti-inflammatory, wound healing and in vivo antioxidant properties of the leaves of Ficus amplissima Smith. J Ethno Pharmacol 145:139–145

    Article  CAS  Google Scholar 

  • Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287

    Article  CAS  Google Scholar 

  • Beckers GJM, Spoel SH (2006) Fine-tuning plant defence signalling: salicylate versus jasmonate. Plant Biol 8:1–10

    Article  CAS  Google Scholar 

  • Bharitkar YP, Hazra A, Apoorva Poduri NS, Ash A, Maulik PR, Mondal NB (2014) Isolation, structural elucidation and cytotoxicity evaluation of a new pentahydroxy pimarane diterpenoid along with other chemical constituents from Aerva lanata. Nat Prod Res 29:253–261

    Article  Google Scholar 

  • Boobalan S, Kamalanathan D (2019) Spermidine influences enhanced micropropagation and antibacterial activity in Aerva javanica (Burm. F.) Shult. Ind Crops Prod 137:187–196

    Article  CAS  Google Scholar 

  • Boobalan S, Kamalanathan D (2020) Tailoring enhanced production of aervine in Aerva lanata (L.) Juss. Ex Schult by Agrobacterium rhizogenes- mediated hairy root cultures. Ind Crops Prod 155:112814

  • Bouaziz F, Koubaa M, Barba FJ, Roohinejad S, Chaabouni SE (2016) Antioxidant properties of water-soluble gum from flaxseed hulls. Antioxidants 5:1–10

    Article  Google Scholar 

  • Chen HP, Xu LL (2005) Isolation and characterization of a novel chitosan-binding protein from non-heading Chinese cabbage leaves. J Integr Plant Biol 47:452–456

    Article  CAS  Google Scholar 

  • Chen SL, Yu H, Luo HM, Wu Q, Li CF, Steinmetz A (2016) Conservation and sustainable use of medicinal plants: problems, progress, and prospects. Chin Med 11:1–10

    Article  Google Scholar 

  • Dahmer J, do Carmo G, Mostardeiro MA, Neto AT, da Silva UF, Dalcol II, Morel AF (2019) Antibacterial activity of Discaria americana Gillies ex Hook (Rhamnaceae). J Ethnopharmacolol 239:111635

    Article  Google Scholar 

  • Dai JK, Dan WJ, Li N, Du HT, Zhang JW, Wang JR (2016) Synthesis, in vitro antibacterial activities of a series of 3-N-substituted canthin-6-ones. Bioorg Med Chem Lett 26:580–583

    Article  CAS  Google Scholar 

  • Deepthi S, Satheeshkumar K (2016) Enhanced camptothecin production induced by elicitors in the cell suspension cultures of Ophiorrhiza mungos Linn. Plant Cell Tiss Org Cult 124:483–493

    Article  CAS  Google Scholar 

  • Duran RE, Kilic S, Coskun Y (2019) Melatonin influence on in vitro callus induction and phenolic compound production in sweet basil (Ocimum basilicum L.). In Vitro Cell Dev Biol - Plant 55:468–475

    Article  CAS  Google Scholar 

  • Gabr AMM, Sytar O, Ahmed AR, Smetanska I (2012) Production of phenolic acid and antioxidant activity in transformed hairy root cultures of common Buckwheat (Fagopyrum esculentum M.). Aust J Basic Appl Sci 6:577–586

    CAS  Google Scholar 

  • Girdhar S, Girdhar A, Verma SK, Lather V, Pandita D (2015) Plant derived alkaloids in major neurodegenerative diseases: from animal models to clinical trials. J Ayu Her Med 1:91–100

    Article  Google Scholar 

  • Gonzalez-Lamothe R, Mitchell G, Gattuso M, Diarra MS, Malouin F, Bouarab K (2009) Plant antimicrobial agents and their effects on plant and human pathogens. Int J M Sci 10:3400–3419

    CAS  Google Scholar 

  • Gulcin I, Elias R, Gepdiremen A, Chea A, Topal F (2010) Antioxidant activity of bisbenzylisoquinoline alkaloids from Stephania rotunda: cepharanthine and fangchinoline. J Enzyme Inhib Med Chem 25:44–53

    Article  CAS  Google Scholar 

  • He J, Liu Y, Kang Y, Yang P, Wang Y, Guo J, Huang J (2016) Identification of alkaloids in Stephania hainanensis by liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. Phytochem Anal 27:206–216

    Article  Google Scholar 

  • Hussain H, Al-Harrasi A, Al-Rawahi A, Green IR, Gibbons S (2014) Fruitful decade for antileishmanial compounds from 2002 to late 2011. Chem Rev 114:10369–10428

    Article  CAS  Google Scholar 

  • Iriti M, Faoro F (2009) Chitosan as a MAMP, searching for a PRR. Plant Signal Behav 4:66–68

    Article  CAS  Google Scholar 

  • Izumi CM, Yoshiyuki M, Misugi U (2009) Integration of ROS and hormone signaling. In. Reactive oxygen species in plant signaling: Luis A. del Rio, Alain Puppo, Springer Dordrecht Heidelberg London New York. pp 30

  • Joel JS, Sheena O, Martins O, Onyemauche N, Emmanuel A (2017) Comparative antioxidant capacity of aqueous and ethanol fruit extracts of Tetrapleura tetraptera. Res J Biol Sci 17:185–193

    CAS  Google Scholar 

  • Johnston JW, Dussert S, Gale S, Nadarajan J, Harding K, Benson EE (2006) Optimization of the azinobis-3-ethyl-benzothiazoline-6-sulphonic acid radical scavenging assay for physiological studies of total antioxidant activity in woody plant germplasm. Plant Physiol Biochem 44:193–201

    Article  CAS  Google Scholar 

  • Kagan IA, Flythe MD (2014) Thin-layer chromatographic (TLC) separations and bioassays of plant extracts to identify antimicrobial compounds. J Vis Exp 85:1–8

  • Kang SM, Min JY, Kim YD, Karigar CS, Kim SW, Goo GH, Choi MS (2009) Effect of biotic elicitors on the accumulation of bilobalide and ginkgolides in Ginkgo biloba cell cultures. J Biotechnol 139:84–88

    Article  CAS  Google Scholar 

  • Karajibani M, Montazerifar F, Feizabad KA (2017) Study of oxidants and antioxidants in addicts. Int J High Risk Behav Addict 6:e3505

    Google Scholar 

  • Karwasara VS, Dixit VK (2013) Culture medium optimization for camptothecin production in cell suspension cultures of Nothapodytes nimmoniana (J. Grah.) Mabberley. Plant Biotechnol Rep 7:357–369

    Article  Google Scholar 

  • Karwasara VS, Tomar P, Dixit VK (2011) Influence of fungal elicitation on glycyrrhizin production in transformed cell cultures of Abrus precatorius L. Pharmacogn Mag 7:307–313

    Article  CAS  Google Scholar 

  • Keerthanaa T, Boobalan S, Kamalanathan D, Karunakaran G, Sudha KG, Aarthi M, Prasanna Rajeshkumar M (2022) Elicitation of apigenin in green leafy vegetable plants and its molecular docking evaluation for effective anticancer applications. Plant Cell Tiss Org Cult 1–20

  • Keng CL, Wei AS, Bhatt A (2010) Elicitation effect on cell biomass and production of alkaloids in cell suspension culture of the tropical tree Eurycoma longifolia. Res J Costa Rican Dist Edu Univ 2:239–244

    Google Scholar 

  • Kranner I, Minibayeva FV, Beckett RP, Seal CE (2010) What is stress? Concepts, definitions and applications in seed science. New Phytol 188:655–673

    Article  CAS  Google Scholar 

  • Kubes J, Tumova L, Martin J, Vildova A, Hendrychova H, Sojkova K (2014) The production of isoflavonoids in Genista tinctoria L. cell suspension culture after abiotic stressors treatment. Nat Prod Res 28:2253–2263

    Article  CAS  Google Scholar 

  • Kundu A, Jawali N, Mitra A (2012) Shikimate pathway modulates the elicitor-stimulated accumulation of fragrant 2-hydroxy-4- methoxybenzaldehyde in Hemidesmus indicus roots. Plant Physiol Biochem 56:104–108

    Article  CAS  Google Scholar 

  • Kwiecien I, Szydłowska A, Kawka B, Beerhues L, Ekiert H (2015) Accumulation of biologically active phenolic acids in agitated shoot cultures of three Hypericum perforatum cultivars: ‘Elixir’, ‘Helos’ and ‘Topas.’ Plant Cell Tiss Org Cult 123:273–281

    Article  CAS  Google Scholar 

  • Li LS, Chiroma SM, Hashim T, Adam SK, Moklas MAM, Yusuf Z, Rahman SA (2020) Antioxidant and anti-inflammatory properties of Erythroxylum cuneatum alkaloid leaf extract. Heliyon 6:e04141

    Article  Google Scholar 

  • Mahendran D, Kavi Kishor PB, Sreeramanan S, Venkatachalam P (2018) Enhanced biosynthesis of colchicine and thiocolchicoside contents in cell suspension cultures of Gloriosa superba L. exposed to ethylene inhibitor and elicitors. Ind Crops Prod 120:123–130

    Article  CAS  Google Scholar 

  • Matuszewska A, Jaszek M, Stefaniuk D, Ciszewski T, Matuszewski Ł (2018) Anticancer, antioxidant, and antibacterial activities of low molecular weight bioactive sub fractions isolated from cultures of wood degrading fungus Cerrena unicolor. PLoS ONE 13:e0197044

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Natarajan D, Kamalanathan D (2012) Screening of antibacterial potential of leaves and leaf derived callus extracts of Solanum trilobatum L. an important medicinal plant. J Pharm Res 5:825–827

    Google Scholar 

  • Nazir M, Tungmunnithum D, Bose S, Drouet S, Garros L, Giglioli-Guivarch N, Abbasi BH, Hano C (2019) Differential production of phenylpropanoid metabolites in callus cultures of Ocimum basilicum L. with distinct in vitro antioxidant activities and in vivo protective effects against UV stress. J Agric Food Chem 67:1847–1859

    Article  CAS  Google Scholar 

  • Norozi A, Hosseini B, Jafari M, Farjaminezhad M, Palazon J (2019) Enhanced h6h transcript level, antioxidant activity and tropane alkaloid production in Hyoscyamus reticulatus L. hairy roots elicited by acetylsalicylic acid. Plant Biosyst - Int J Dealing Aspects Plant Biol 153:360–366

    Google Scholar 

  • Oboh G, Adedayo BC, Adetola MB, Oyeleye IS, Ogunsuyi OB (2020) Characterization and neuroprotective properties of alkaloid extract of Vernonia amygdalina Delile in experimental models of Alzheimer’s disease. Drug Chem Toxicol 16:1–10

    Google Scholar 

  • Orlita A, Sidwa-Gorycka M, Paszkiewicz M, Malinski E, Kumirska J, Siedlecka EM, Lojkowska E, Stepnowski P (2008) Application of chitin and chitosan as elicitors of coumarins and furoquinolone alkaloids in Ruta graveolens L. (common rue). Biotechnol Appl Biochem 51:91–96

    Article  CAS  Google Scholar 

  • Othman WNN, Sivasothy Y, Liew SY, Mohamad J, Nafiah MA, Ahmad K, Litaudon M, Awang K (2017) Alkaloids from Cryptocarya densiflora Blume (Lauraceae) and their cholinesterase inhibitory activity. Phytochem Lett 21:230–236

    Article  Google Scholar 

  • Patel AK, Phulwaria M, Rai MK, Gupta AK, Shekhawat S, Shekhawat NS (2014) In vitro propagation and ex vitro rooting of Caralluma edulis (Edgew.) Benth. & Hook. f. An endemic and endangered edible plant species of the Thar Desert. Sci Hortic 165:175–180

    Article  CAS  Google Scholar 

  • Patil AS, Kale AS, Patil SR (2019) Studies on stages of somatic embryogenesis in Nothapodytes nimmoniana and its quantitative evaluation of camptothecin content. Hort Int J 3:24–29

    Google Scholar 

  • Pitta-Alvarez SI, Spollansky TC, Giulietti AM (2000) The influence of different biotic and abiotic elicitors on the production and profile of tropane alkaloids in hairy root cultures of Brugmansia candida. Enzyme Microb Technol 26:252–258

    Article  CAS  Google Scholar 

  • Prabakaran M, Ragavendran C, Kamalanathan D, Natarajan D (2014) Studies on Comparative antibacterial activity of wild and micropropagated leaves extracts from Momordica charantia L. (Cucurbitacaeae). J Pharma Res 8:1729–1737

    CAS  Google Scholar 

  • Prieto P, Pineda M, Aguilar M (1999) Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 269:337–341

    Article  CAS  Google Scholar 

  • Prior RL, Wu X, Schaich K (2005) Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem 53:4290–4302

    Article  CAS  Google Scholar 

  • Priya CL, Kumar G, Karthik L, Rao KVB (2010) Antioxidant activity of Achyranthes aspera Linn stem extracts. Pharmacologyonline 2:228–237

    Google Scholar 

  • Pulido R, Bravo L, Saura-Calixto F (2000) Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. J Agric Food Chem 48:3396–3402

    Article  CAS  Google Scholar 

  • Rahimi S, Kim YJ, Yang DC (2015) Production of ginseng saponins: elicitation strategy and signal transductions. Appl Microbiol Biotechnol 99:6987–6996

    Article  CAS  Google Scholar 

  • Ramirez-Estrada K, Vidal-Limon H, Hidalgo D, Moyano E, Golenioswki M, Cusido RM, Palazon J (2016) Elicitation, an effective strategy for the biotechnological production of bioactive high-added value compounds in plant cell factories. Molecules 21:182–205

    Article  Google Scholar 

  • Rao KN, Venkatachalam SR (2000) Inhibition of dihydrofolate reductase and cell growth activity by the phenanthroindolizidine alkaloids pergularinine andtylophorinidine: the in vitro cytotoxicity of these plant alkaloids and their potential as antimicrobial and anticancer agents. Toxicol In Vitro 14:53–59

  • Rasouli D, Werbrouck S, Maleki B, Jafary H, Schurdi-Levraud V (2021) Elicitor-induced in vitro shoot multiplication and steviol glycosides production in Stevia rebaudiana. South African J Bot 137:265–271

    Article  CAS  Google Scholar 

  • Sanchez-Sampedro MA, Fernandez-Tarrago J, Corchete P (2005) Yeast extract and methyl jasmonate-induced silymarin production in cell cultures of Silybum marianum (L.) Gaertn. J Biotechnol 119:60–69

    Article  CAS  Google Scholar 

  • Semenya CJ, Maseko RB, Gololo SS (2018) Comparative qualitative phytochemical analysis of the different parts of Barleria dinteri (Oberm): a contribution to sustainable use of the plant species. J Pharma Chem Biol Sci 6:52–59

    CAS  Google Scholar 

  • Senizza B, Rocchetti G, Okur MA, Zengin G, Yıldıztugay EAK, Montesano D, Lucini L (2020) Phytochemical profile and biological properties of Colchicum triphyllum (Meadow Saffron). Foods 9:457–471

    Article  CAS  Google Scholar 

  • Shekhawat MS, Manokari M, Revathi J (2017) In vitro propagation and ex vitro rooting of Aerva lanata (L.) Juss. ex Schult.: a rare medicinal plant. Ind J Plant Physiol 22:40–47

    Article  Google Scholar 

  • Shekhawat MS, Shekhawat Harish NS, Ram K, Phulwaria M, Gupta AK (2011) High frequency plantlet regeneration from nodal segment culture of female Momordica dioica (Roxb.). J Crop Sci Biotechnol 14:133–137

    Article  Google Scholar 

  • Shubhra RD, Polash SA, Saha T, Hasan A, Hossain S, Islam Z, Sarker SR (2019) Investigation of the phytoconstituents and antioxidant activity of Diospyros malabarica fruit extracts. Adv Biosci Biotechnol 10:431–454

    Article  CAS  Google Scholar 

  • Siveen KS, Kuttan G (2012) Modulation of humoral immune responses and inhibition of proinflammatory cytokines and nitric oxide production by 10-methoxycanthin-6-one. Immunopharmacol Immunotoxicol 34:116–125

    Article  CAS  Google Scholar 

  • Smetanska I (2008) Production of secondary metabolites using plant cell cultures. Food Biotechnol 111:187–228

    Article  CAS  Google Scholar 

  • Sparzak B, Dybowski F, Krauze-Baranowska M (2015) Analysis of Securinega-type alkaloids from Phyllanthus glaucus biomass. Phytochem Lett 11:353–357

    Article  CAS  Google Scholar 

  • Sreeranjini S, Siril EA (2014) Field performance and genetic fidelity evaluation of micropropagated Morinda citrifolia L. Indian J Biotechnol 13:121–130

    CAS  Google Scholar 

  • Tarun B, Aseesh P, Bhatt ID, Rawal RS (2020) Optimized microwave assisted extraction (MAE) of alkaloids and polyphenols from Berberis roots using multiple-component analysis. Sci Rep 10:1–10

    Google Scholar 

  • Touani FK, Seukep AJ, Djeussi DE, Fankam AG, Noumedem JA, Kuete V (2014) Antibiotic-potentiation activities of four Cameroonian dietary plants against multidrug-resistant Gram-negative bacteria expressing efflux pumps. BMC Complement Altern Med 14:1–8

    Article  Google Scholar 

  • Udomsuk L, Jarukamjorn K, Tanaka H, Putalun W (2011) Improved isoflavonoid production in Pueraria candollei hairy root cultures using elicitation. Biotechnol Lett 33:369–374

    Article  CAS  Google Scholar 

  • Varutharaju K, SoundarRaju C, Thilip C, Aslam A, Shajahan A (2014) High efficiency direct shoot organogenesis from leaf segments of Aerva lanata (L.) Juss. Ex Schult by using thidiazuron. Sci World J 652919:1–6

    Article  Google Scholar 

  • Veerashree V, Anuradha CM, Kumar V (2012) Elicitor-enhanced production of gymnemic acid in cell suspension cultures of Gymnema sylvestre R. R Br. Plant Cell Tiss Org Cult 108:27–35

    Article  CAS  Google Scholar 

  • Wansi JD, Devkota KP, Tshikalange E, Kuete V (2013) Alkaloids from the medicinal plants of Africa. In Medicinal plant research in Africa (pp. 557–605). Elsevier

  • Wu Q, Er-bu A, Liang X, Luan S, Wang Y, Yin Z, He C, Yin L, Zou Y, Li L, Song X (2020) Evaluation of antioxidant and anticancer activities of naphthoquinones enriched ethanol extracts from the roots of Onosma hookeri Clarke. var. longiforum Duthie. Food Sci Nutr 8:4320–4329

    Article  CAS  Google Scholar 

  • Yamunadevi M, Wesely EG, Johnson M (2011) A chromatographic study on the glycosides of Aerva lanata L. Chinese J Nat Med 9:210–214

    CAS  Google Scholar 

  • Yasir M, Sultana B, Amicucci M (2016) Biological activities of phenolic compounds extracted from Amaranthaceae plants and their LC/ESI-MS/MS profiling. J Funct Foods 26:645–656

    Article  CAS  Google Scholar 

  • Zahari A, Ablat A, Sivasothy Y, Mohamad J, Choudhary MI, Awang K (2016) In vitro antiplasmodial and antioxidant activities of bisbenzylisoquinoline alkaloids from Alseodaphne corneri Kosterm. Asian Pac J Trop Med 9:328–332

    Article  CAS  Google Scholar 

  • Zakaria R, Allen KJ, Koplin JJ, Roche PJ, Greaves RF (2016) Advantages and challenges of dried blood spot analysis by mass spectrometry across the total testing process. EJIFCC 27:288–317

    CAS  Google Scholar 

  • Zapesochnaya G, Kurkin V, Okhanov V, Miroshnikov A (1991) Canthin-6-one and β-carboline alkaloids from Aerva lanata. Planta Med 58:192–196

    Article  Google Scholar 

  • Zeynali Z, Hosseini B, Rezaei E (2016) Effect of elicitation on antioxidant activity and production of tropane alkaloids in Hyoscyamus reticulatus hairy root cultures. Res J Pharmacog 3:43–53

    CAS  Google Scholar 

  • Zhang C, Mei X, Yu L, Liu X (2000) Initiation, growth and taxol production of Taxus chinensis embryo-derived cell line. J Huazhong Univ Sci Technolog 28:60–63

    Google Scholar 

  • Zhang DY, Wan Y, Hao JY, Hu RZ, ChenC YXH, Zhao WG, Liu ZY, Li L (2018) Evaluation of the alkaloid, polyphenols, and antioxidant contents of various mulberry cultivars from different planting areas in eastern China. Ind Crops Prod 122:298–307

    Article  CAS  Google Scholar 

  • Zhang Y, Liu YB, Li Y, Ma SG, Li L, Qu J, Zhang D, Chen XG, Jiang JD, Yu SS (2013) Sesquiterpenes and alkaloids from the roots of Alangium chinense. J Nat Prod 76:1058–1063

    Article  CAS  Google Scholar 

  • Zhao F, Dai JK, Li D, Wang SJ, Wang JR (2016) Synthesis and evaluation of ester derivatives of 10-hydroxycanthin-6-one as potential antimicrobial agents. Molecules 21:390–413

    Article  Google Scholar 

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Acknowledgements

The corresponding author with gratitude acknowledges the Science and Engineering Research Board (SERB, New Delhi) EMEQ—Empowerment and Equity Opportunities for Excellence in Science Scheme for their financial assistance for this research work (SERB letter no. EEQ/2016/000233, Dt: 14 March 2017). The authors extend their deepest gratitude to the Department of Biotechnology from K. S. Rangasamy College of Arts and Science (Autonomous), Tiruchengode, for providing the necessary facilities to carry out this research work efficiently.

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  1. Department of Biotechnology, K.S. Rangasamy College of Arts and Science (Autonomous), Tiruchengode, Namakkal, Tamil Nadu, 637215, India

    Selvakumar Boobalan & Desingu Kamalanathan

  2. Department of Biotechnology, Sona College of Arts and Science, Salem, Tamil Nadu, 636005, India

    Desingu Kamalanathan

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Selvakumar Boobalan: investigation; methodology; data curation; formal analysis; writing—original draft, and editing. Desingu Kamalanathan: conceptualization; funding acquisition; supervision; validation; writing review and editing.

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Boobalan, S., Kamalanathan, D. Elicitor-induced production of aervine in adventitious shoot cultures of Aerva lanata (L.) Juss. Ex Schult. and its biological applications. In Vitro Cell.Dev.Biol.-Plant 58, 972–988 (2022). https://doi.org/10.1007/s11627-022-10283-8

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