Abstract
Sesame (Sesamum indicum L.; 2n = 26) is an ancient oil-yielding crop known for its valuable nutrients such as essential fatty acids, proteins, and medicinally important lignans. Recalcitrant nature of sesame limits the use of modern biotechnology for its genetic improvement. For the development of an efficient in vitro regeneration protocol, the cotyledon (de-embryonated) explants were used for the development of adventitious shoots. In the present study, Murashige and Skoog (MS) medium with 25 μM BAP (6-benzylaminopurine) and 2 μM IAA (indole-3-acetic acid) showed high regeneration frequency in both GT-3 (33.3 ± 1.1%) and GT-4 (30 ± 0.0%) varieties within 6 wk of culture. Addition of 20 μM thidiazuron (TDZ) showed further improvement in regeneration frequency in both GT-3 (63.3 ± 1.1%) and GT-4 (26.7 ± 1.1%) varieties. In another experiment, 0-d-old cotyledons inoculated on MS medium supplemented with 4 μM abscisic acid (ABA) showed the highest shoot regeneration in GT-3 (83.3 ± 3.3%) and GT-4 (70.0 ± 0.1%) varieties after 6 wk. The regenerated shoots transferred on MS medium with 5 μM BAP and 2 μM IAA or 1 μM GA3 (gibberellic acid) showed good elongation. Maximum root induction frequency (70.0 ± 2.3%) was achieved on MS medium with 2 μM α-naphthalene acetic acid (NAA) after 4 wk. The sesame plants in green house showed normal vegetative growth and flowering. The regeneration protocol can be used for the transformation of sesame crop for genetic improvement.
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References
Al-Shafeay AF, Ibrahim AS, Nesiem MR, Tawfik MS (2011) Establishment of regeneration and transformation system in Egyptian sesame (Sesamum indicum L.) cv Sohag 1. GM Crops 2:182–192
Baskaran P, Jayabalan N (2006) In vitro mass propagation and diverse callus orientation on Sesamum indicum L.-an important oil plant. J Agric Technol 2:259–269
Bedigian D (2004) History and lore of sesame in Southwest Asia. Econ Bot 58:329–353
Chi GL, Pua EC (1989) Ethylene inhibitors enhanced de novo shoot regeneration from cotyledons of Brassica campestris ssp. chinensis (Chinese cabbage) in vitro. Plant Sci 64:243–250
Chowdhury S, Basu A, Kundu S (2014) A new high-frequency Agrobacterium-mediated transformation technique for Sesamum indicum L. using de-embryonated cotyledon as explant. Protoplasma 25:1175–1190
Debnath AJ, Gangopadhyay G, Basu D, Sikdar SR (2018) An efficient protocol for in vitro direct shoot organogenesis of Sesamum indicum L. using cotyledon as explant. 3 Biotech 8:1–13
Gangopadhyay G, Poddar R, Gupta S (1998) Micropropagation sesame (Sesamum indicum L) by in vitro multiple shoot production from nodal explants. Phytomorphology: Int J Plant Morphol 48:83–89
Gayatri T, Basu A (2020) Development of reproducible regeneration and transformation system for Sesamum indicum. Plant Cell Tiss Org Cult 143:441–456
Honnale H, Rao S (2013) Direct somatic embryogenesis in Sesamum indicum (L.) CV-E8 from cotyledon and hypocotyl explants. Int J Appl Biol Pharm 4:120–127
Islam F, Gill RA, Ali B, FarooqMA, Xu L, Najeeb U, Zhou W (2016) Sesame. In: Gupta SR (ed) Breeding oilseed crops for sustainable production: opportunities and constraints, Academic Press, p 135–147
Kafiriti E, Mponda O (2010) Growth and production of sesame. In: Soils, plant growth and crop production, Encyclopedia of life support systems (EOLSS), United Kingdom
Kulkarni VV, Ranganatha CN, Shankergoud I (2017) Interspecific crossing barriers in sesame (Sesamum indicum L.). Int J Curr Microbiol Appl Sci 6:4894–4900
Lokesha R, Rahaminsab J, Ranganatha AR, Dharmaraj PS (2012) Whole plant regeneration via adventitious shoot formation from de-embryonated cotyledon explants of sesame (Sesamum indicum L.). World J Sci Technol 2:47–51
Malaghan SV, Lokesha R, Savitha R, Ranganatha AR (2013) Adventitious shoot regeneration in sesame (Sesamum indicum L.) (Pedaliaceae) via deembryonated cotyledonary explants. Research J Biol 1:31–35
Mary RJ, Jayabalan N (1997) Influence of growth regulators on somatic embryogenesis in sesame. Plant Cell Tiss Org Cult 49:67–70
Miraj S, Kiani S (2016) Bioactivity of Sesamum indicum: a review study. Der Pharm Lett 8:328–334
Mithila J, Hall JC, Victor JMR, Saxena PK (2003) Thidiazuron induces shoot organogenesis at low concentration and somatic embryogenesis at high concentration on leaf and petiole explants of African violet (Saintpaulia ionantha WEndl). Plant Cell Rep 21:408–414
Moazzami AA, Andersson RE, Kamal-Eldin A (2006) HPLC analysis of sesaminol glucosides in sesame seeds. J Agric Food Chem 54:633–638
Mohiuddin AKM, Chowdhury MKU, Abdullah ZC, Napis S (1997) Influence of silver nitrate (ethylene inhibitor) on cucumber in vitro shoot regeneration. Plant Cell Tiss Org Cult 51:75–78
Morris JB (2002) Food, industrial, nutraceutical, and pharmaceutical uses of sesame genetic resources. In: Janick J, Whipkey A (eds) Trends in new crops and new uses: ASHS Press, Alexandria, p 153–156
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Pathak N, Rai AK, Saha S, Walia S, Sen SK, Bhat KV (2014) Quantitative dissection of antioxidative bioactive components in cultivated and wild sesame germplasm reveals potentially exploitable wide genetic variability. J Crop Sci Biotechnol 17:127–139
Pelah D, Kaushik RA, Mizrahi Y, Sitrit Y (2002) Organogenesis in the vine cactus Selenicereus megalanthus using thidiazuron. Plant Cell Tissue Organ Cult 71:81–84
Purnhauster L, Medgyesy P, Czako M, Dix PJ, Marton L (1987) Stimulation of shoot regeneration in Triticum aestivum and Nicotiana plumbaginifolia Viv. tissue cultures using the ethylene inhibitor AgNO3. Plant Cell Rep 6:1–4
Raja A, Jayabalan N (2010) Callus induction and plantlet regeneration from leaf explant of sesame (Sesamum indicum L. Cv. SVPR - 1). J Swamy Bot Club 27:93–98
Ram R, Catlin D, Romero J, Cowley C (1990) Sesame: new approaches for crop improvement. In: Janick J, Simon JE (eds) Advances in new crops. Timber Press, Portland, p 225–228
Rao KR, Kishor PBK, Vaidyanath K (2002) Biotechnology of sesame-an oil seed crop. Plant Cell Biotechnol Mol Biol 3:101–110
Saravanan S, Nadarajan N (2005) Effect of media supplements on in vitro response of sesame (Sesamum indicum L.) genotypes. Res J Agric Biol Sci 1:98–100
Seo HY, Kim YJ, Park TI, Kim HS, Yun HS (2007) High-frequency plant regeneration via adventitious shoot formation from de embryonated cotyledon explants of Sesamum indicum L. In Vitro Cell Dev Biol - Plant 43:209–214
Shukla PS, Das AK, Jha B, Agarwal PK (2014) High-frequency in vitro shoot regeneration in Cucumis sativus by inhibition of endogenous auxin. In Vitro Cell Dev Biol - Plant 50:729–737
Songstad DD, Duncan DR, Widholm JM (1988) Effect of l-aminocyclopropane-l-carboxylic acid, silver nitrate, and norbornadiene on plant regeneration from maize callus cultures. Plant Cell Rep 7:262–265
Su YH, Tang LP, Zhao XY, Zhang XS (2021) Plant cell totipotency: insights into cellular reprogramming. J Integr Plant Biol 63:228–240
Tiwari S, Kumar S, Gontia I (2011) Biotechnological approaches for sesame (Sesamum indicum L.) and Niger (Guizotia abyssinica Lf Cass.). Asia Pac J Mol Biol 19:2–9
Van Staden J, Zazimalova E, George EF (2008) Plant growth regulators II: cytokinins, their analogues and antagonists. In: George EF, Hall MA, De Klerk GJ (eds) Plant propagation by tissue culture, vol I the background, 3rd edn. Springer, Dordrecht, p 205–226
Wadeyar B, Lokesha R (2011) Studies on high frequency shoot regeneration in sesame (Sesamum indicum L.). Plant Tissue Cult Biotech 21:45–52
Were B, Gudu S, Onkware AO, Carlsson AS,Welander M (2006) In vitro regeneration of sesame (Sesamum indicum L.) from seedling cotyledon and hypocotyl explants. Plant Cell Tiss Org Cult 85:235–239
Yadav M, Chaudhary D, Sainger M, Jaiwal PK (2010) Agrobacterium tumefaciens-mediated genetic transformation of sesame (Sesamum indicum L.). Plant Cell Tiss Org Cult 103:377–386
Zimik M, Arumugam N (2017) Induction of shoot regeneration in cotyledon explants of the oilseed crop Sesamum indicum L. J Genet Eng Biotechnol 15:303–308
Funding
CSIR-CSMCRI Communication No. 175/ 2021. PR is supported by UGC-JRF fellowships. PR is thankful for AcSIR for enrolment in Ph.D. P.A. acknowledges the DSTWOS-A scheme for financial assistance. The authors are thankful to the Department of Science and Technology DST (SERB-CRG/2018/000145) and Council of Scientific and Industrial Research (CSIR), New Delhi, India for financial assistance.
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PKA conceived the idea and guided the activity; PKA and PA supervised the experiments; PR carried out the experiments and data analysis. PKA, PA, and DRG are involved in manuscript writing.
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Rajput, P., Agarwal, P., Gangapur, D.R. et al. Development of a high-frequency adventitious shoot regeneration using cotyledon explants of an important oilseed crop Sesamum indicum L.. In Vitro Cell.Dev.Biol.-Plant 58, 470–478 (2022). https://doi.org/10.1007/s11627-021-10247-4
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DOI: https://doi.org/10.1007/s11627-021-10247-4